Recombinant Mouse M-CSF Protein, CF 416-ML-010/CF from R&D Systems, a Bio-Techne Brand

Product Specs
ItemRecombinant Mouse M-CSF Protein, CF
CompanyR&D Systems, a Bio-Techne Brand
Price $292.00Supplier Page View Company Product Page
Catalog Number416-ML-010/CF
SpeciesMouse
FormatLyophilized from a 0.2 µm filtered solution in PBS
Size10 ug (also 50 ug)
Molecule NameM-CSF
Purity>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Molecular WeightObserved: 29 kDa, reducing conditions
Theoretical: 26 kDa (monomer)
ApplicationsBioactivity
Conjugate/TagUnconjugated
Source/Expression SystemE. coli-derived Recombinant Mouse M-CSF Protein, CF
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Full Gene Namecolony stimulating factor 1 (macrophage)
NCBI Gene AliasesBAP025, Csfm, MCSF, Mhdabap25, PG-M-CSF, op

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Complement receptor C3ar1 deficiency does not alter brain structure or functional connectivity across early life developmentBrain CommunicationsOctober 30, 2025Hanna Lemmik, Eugene Kim, Eilidh MacNicol, Davide Maselli, Michel Bernanos, Zhuoni Li, Dauda Abdullahi, Esther Walters, Maria Elisa Serrano Navacerrada, Wuding Zhou, et al.supplemented with 50 ng/ml recombinant mouse macrophage colony-stimulating factor (M-CSF, R&D Systems, #416-ML-010/CF), 1% penicillin, 1% streptomycin, and 10% heat-inactivated foetal bovine serum (Sigma, #F9665-50 ml). Cells

(2) An interleukin-27-centered cytokine circuit regulates macrophage and T cell interactions in autoimmune diabetesiScienceOctober 17, 2025Ashley E. Ciecko, Rabia Nabi, Amber Drewek, David M. Schauder, Pavel N. Zakharov, Xiaoxiao Wan, Scott M. Lieberman, Weiguo Cui, Martin J. Hessner, Chien-Wei Lin, et al.Zombie NIR Fixable Viability Kit,BioLegend,Cat#423105 Recombinant mouse IFN-gamma,R&D SYSTEMs,Cat#485-MI-100/CF Recombinant mouse M-CSF,R&D Systems,Cat#416-ML-010/CF ,, Critical Commercial Assays,Critical Commercial Assays,Critical Commercial Assays ,, Anti-CD3ε

(3) Delaying pyroptosis with an AI-screened gasdermin D pore blocker mitigates inflammatory responseNature immunologyOctober 1, 2025Sun J, Yang J, Tao J, Yang Y, Wang R, Zhang H, Liu W, Zhao S, Shao R, He Y, et al.resuspended in DMEM/F12 containing 10 ng ml−1 recombinant mouse macrophage colony-stimulating factor (416-ML-010/CF, R&D Systems). The medium was refreshed every other day, and the cells were cultured until day 6 to

(4) Modular inflammation network discovery from large-scale phenotypic screening in genetically heterogeneous mouse brainsJournal of neuroinflammationSeptember 29, 2025Xiong M, Miosge LA, Correa-Ospina C, Yan CMY, Cripps T, Bauernfried S, Wang Y, Crow M, Morris LX, Andrews TD, et al.(125 rpm for 2 hr), collected and plated at 40k/well in the presence of 20 ng/mL CSF-1 (R&D systems, 416-ML-010/CF). One day later, microglia were infected with AAV at MOI of 400,000. 5 days post infection, Propidium

(5) Type I interferon protects against bone loss in periodontitis by mitigating an interleukin (IL)-17-neutrophil axisLife SciencesJune 15, 2025Jinmei Zhang, Qiong Ding, Angela X. Wang, Maoxuan Lin, Ning Yu, Kevin Moss, Megumi A. Williamson, Di Miao, Julie T. Marchesan, Erliang Zeng, et al.105 cells per well in the presence of 30 ng/ml macrophage colony-stimulating factor (M-CSF, catalog # 416-ML-010/CF, R&D Systems) in the culture medium to allow differentiation into primary macrophages. After three days

(6) Protocol for the generation and assessment of functional macrophages from mouse bone marrow cellsSTAR ProtocolsJune 1, 2025Andrea Scafidi, Frida Lind-Holm Mogensen, Alessandro MichelucciHEPES (1M),Fisher,Cat# 11560496; CAS: 7365-45-9 "Recombinant mouse M-CSF protein, 10 μg",Bio-Techne (R&D Systems),Cat# 416-ML-010/CF "Fetal bovine serum, qualified, Brazil",Fisher (Gibco),Cat# 10270-106 Metformin hydrochloride,Merck,Cat#

(7) Innate immune training of osteoclastogenesis promotes inflammatory bone loss in miceDevelopmental CellFebruary 19, 2025Nora Haacke, Hui Wang, Shu Yan, Marko Barovic, Xiaofei Li, Kosuke Nagai, Adelina Botezatu, Aikaterini Hatzioannou, Bettina Gercken, Giulia Trimaglio, et al.15140122 RBC Lysis Buffer (10X),eBioscience,Cat# 00-4300-54 Recombinant Mouse M-CSF Protein,R&D systems,Cat# 416-ML-010/CF Recombinant Mouse TRANCE/RANK L/TNFSF11,R&D systems,Cat# 462-TEC-010/CF RPMI 1640 Medium,Gibco,Cat#

(8) TGF-β3 Restrains Osteoclastic Resorption Through AutophagyBioengineering (Basel)November 28, 2024Hui Liao, Yiqin Pan, Yiming Liu, Yuxiao Li, Shiyi Huang, Shan Ding, Qi Xiangwere cultured until the second generation for use in the experiment. The control group needed M-CSF (416-ML-010/CF, R&D Systems, Minneapolis, MN, USA, USD, 25 ng/mL) added to the culture medium, the RANKL group needed

(9) Cellular and molecular determinants of bacterial burden in leprosy granulomas revealed by single-cell multimodal omicsEBioMedicineOctober 1, 2024Mi Z, Wang Z, Wang Y, Xue X, Liao X, Wang C, Sun L, Lin Y, Wang J, Guo D, et al.

(10) Identification of tyrosine brominated extracellular matrix proteins in normal and fibrotic lung tissuesRedox biologyMay 1, 2024Cruz LC, Habibovic A, Dempsey B, Massafera MP, Janssen-Heininger YMW, Lin MJ, Hoffman ET, Weiss DJ, Huang SK, van der Vliet A, et al.

(11) PARP14 Contributes to the Development of the Tumor-Associated Macrophage PhenotypeInternational Journal of Molecular SciencesMarch 22, 2024Isotta Sturniolo, Csongor Váróczy, Zsolt Regdon, Anett Mázló, Szabolcs Muzsai, Attila Bácsi, Giorgia Intili, Csaba Hegedűs, Mark R. Boothby, Jacob Holechek, et al.[31] were from the Boothby Lab (Vanderbilt University, Nashville, TN, USA). Recombinant mouse M-CSF (416-ML-010/CF) was purchased from R&D Systems (Minneapolis, MN, USA). PJ34 (2-(dimethylamino)-N-(6-oxo-5H-phenanthridin-2-yl)acetamide

(12) GLI1 facilitates collagen-induced arthritis in mice by collaborative regulation of DNA methyltransferaseseLifeNovember 6, 2023Ge G, Guo Q, Zhou Y, Li W, Zhang W, Bai J, Wang Q, Tao H, Wang W, Wang Z, et al.

(13) Obacunone targets macrophage migration inhibitory factor (MIF) to impede osteoclastogenesis and alleviate ovariectomy-induced bone lossJournal of advanced researchNovember 1, 2023He J, Zheng L, Li X, Huang F, Hu S, Chen L, Jiang M, Lin X, Jiang H, Zeng Y, et al.

(14) C3a-C3aR signaling is a novel modulator of skeletal homeostasisBone reportsJune 1, 2023Kuhn MB, VandenBerg HS, Reynolds AJ, Carson MD, Warner AJ, LaRue AC, Novince CM, Hathaway-Schrader JD.

(15) GSTP1-mediated S-glutathionylation of Pik3r1 is a redox hub that inhibits osteoclastogenesis through regulating autophagic fluxRedox biologyMay 1, 2023Ji X, Hong J, Yang W, Yao M, Wang J, Jiang G, Wang Y, Li C, Lin J, Mou H, et al.

(16) Cedrol, a Ginger-derived sesquiterpineol, suppresses estrogen-deficient osteoporosis by intervening NFATc1 and reactive oxygen speciesInternational immunopharmacologyApril 1, 2023Xu C, Jin SQ, Jin C, Dai ZH, Wu YH, He GL, Ma HW, Xu CY, Fang WL.

(17) Obacunone inhibits RANKL/M-CSF-mediated osteoclastogenesis by suppressing integrin- FAK-Src signalingCytokineApril 1, 2023Hu H, Wang X, Huang Y, He B, Zhu J, Sun K, Deng C, Guo Y, Hao D, Jian B.

(18) Activation of TRPV1 receptor facilitates myelin repair following demyelination via the regulation of microglial functionActa pharmacologica SinicaApril 1, 2023Sun JX, Zhu KY, Wang YM, Wang DJ, Zhang MZ, Sarlus H, Benito-Cuesta I, Zhao XQ, Zou ZF, Zhong QY, et al.

(19) Atf4 regulates angiogenic differences between alveolar bone and long bone macrophages by regulating M1 polarization, based on single-cell RNA sequencing, RNA-seq and ATAC-seq analysisJournal of translational medicineMarch 14, 2023Gu L, Wang Z, Gu H, Wang H, Liu L, Zhang WB.

(20) Probucol suppresses osteoclastogenesis via activating Nrf2 signaling and ameliorates ovariectomy-induced bone lossInternational immunopharmacologyMarch 1, 2023Guo J, Ren R, Guo Z, Sun K, He J, Shao J, Wang X.

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2/18/2020In Vitro Bioactivity in Cell Culture Joanna PlastiraA good and basic growth factor for microglia cultures.

M-CSF Protein

M-CSF Protein

M-CSF Protein

Recombinant Mouse M-CSF Protein, CF from R&D Systems, a Bio-Techne Brand

R&D Systems, a Bio-Techne Brand

Citations (578)

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Recombinant Mouse M-CSF Protein, CF from R&D Systems, a Bio-Techne Brand

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