Recombinant Human FGF-9 Protein 273-F9-025 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemRecombinant Human FGF-9 Protein
CompanyR&D Systems, a Bio-Techne Brand
Price $334.00Supplier Page View Company Product Page
Catalog Number273-F9-025
SpeciesHuman
Size25 ug
Molecule NameFGF-9
NCBI Full Gene Namefibroblast growth factor 9
NCBI Gene AliasesHBGF-9, GAF, SYNS3, FGF-9, HBFG-9
FormatLyophilized from a 0.2 µm filtered solution in MOPS, Na2SO4 and EDTA with BSA as a carrier protein
Purity>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
Molecular WeightObserved: Multiple bands between 22-29 kDa, reducing conditions
Theoretical: 23 kDa
ApplicationsBioactivity
Conjugate/TagUnconjugated
Source/Expression SystemSf 21 (baculovirus)-derived Recombinant Human FGF-9 Protein
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 3 months, 2 to 8 °C under sterile conditions after reconstitution

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Rhomboid protease GlpG regulates type 1 pili quality control and virulence in pathogenic E. coliNature CommunicationsDecember 30, 2025Jimmy Lu, Elena Arutyunova, Bridgette Hartley, Jonas Wong, Hyunjae Chung, Lanny Buchan, Heather Armstrong, Sadhna Phanse, Howard S. Young, Eytan Wine, et al.Systems, Cat. #4423) for 4 days followed by recombinant human FGF-9 (200 ng/mL; R&D Systems, Cat. #273-F9-025) and heparin (1 μg/mL, Millipore Sigma, Cat. #H4784) for an additional 3 days in APEL2 medium (Stem Cell Technologies

(2) Spatially patterned kidney assembloids recapitulate progenitor self-assembly and enable high-fidelity in vivo disease modelingCell Stem CellOctober 2, 2025Biao Huang, Pedro Medina, Jincan He, Zipeng Zeng, Sunghyun Kim, Janet Romo, Kari Koppitch, Chennan C. Zhang, Georgina Gyarmati, Yohan Park, et al.proteins","Chemicals, peptides, and recombinant proteins" TTNPB,Cayman Chemical,Cat# 16144 FGF9,R&D Systems,Cat# 273-F9-025 Y-27632,Selleck Chemicals,Cat# S1049 Forskolin,Sigma-Aldrich,Cat# F3917-10MG Aldosterone,Sigma-Aldrich,Cat#

(3) Axial nephron fate switching demonstrates a plastic system tunable on demandNature communicationsAugust 25, 2025Achieng MA, Schnell J, Fausto CC, Csipán RL, Koppitch K, Thornton ME, Grubbs BH, Lindström NO.

(4) Highly parallel production of designer organoids by mosaic patterning of progenitorsCell SystemsJuly 17, 2024Catherine M. Porter, Grace C. Qian, Samuel H. Grindel, Alex J. Hughes(STEMCELL Technologies, 05946) with 1x pen/strep for 5 days. Media was then swapped to 200 ng ml−1 FGF9 (R&D Systems, 273-F9–025) and 1 μg ml−1 heparin (Sigma-Aldrich, H4784) in TeSR-E6 with 1x pen/strep for 5 days to induce NPs.

(5) Stepwise developmental mimicry generates proximal-biased kidney organoidsbioRxiv : the preprint server for biologyJuly 2, 2024Schnell J, Miao Z, Achieng M, Fausto CC, Wang V, Kuyper F, Thornton ME, Grubbs B, Kim J, Lindström NO.

(6) NIBR-LTSi is a selective LATS kinase inhibitor activating YAP signaling and expanding tissue stem cells in vitro and in vivoCell stem cellApril 4, 2024Namoto K, Baader C, Orsini V, Landshammer A, Breuer E, Dinh KT, Ungricht R, Pikiolek M, Laurent S, Lu B, et al.

(7) Ligand bias underlies differential signaling of multiple FGFs via FGFR1eLifeApril 3, 2024Karl K, Del Piccolo N, Light T, Roy T, Dudeja P, Ursachi VC, Fafilek B, Krejci P, Hristova K.bovine serum. Varying amounts of ligands (R&D Systems; FGF4, #235-F4-025; FGF8, #423-F8-025, and FGF9, # 273-F9-025) were added to each well to create a ligand concentration gradient. Cells were incubated with the ligands

(8) Nephron progenitors rhythmically alternate between renewal and differentiation in synchrony with kidney branching morphogenesisbioRxivNovember 25, 2023Sachin N. Davis, Samuel H. Grindel, John M. Viola, Grace Y. Liu, Jiageng Liu, Grace Qian, Catherine M. Porter, Alex J. Hughesmedia was changed to TeSR-E6+, 1 μg/ml heparin (Sigma-Aldrich H4784) and 200 ng/ml FGF9 (R&D Systems 273-F9-025). Media was refreshed every day. On day 10, cells were lifted using Accutase and TeSR-E6+ was used to

(9) Generation of proximal tubule-enhanced kidney organoids from human pluripotent stem cellsNature protocolsNovember 1, 2023Vanslambrouck JM, Tan KS, Mah S, Little MH.

(10) Highly-parallel production of designer organoids by mosaic patterning of progenitorsbioRxiv : the preprint server for biologyOctober 25, 2023Porter CM, Qian GC, Grindel SH, Hughes AJ.

(11) Directed differentiation of ureteric bud and collecting duct organoids from human pluripotent stem cellsNature protocolsAugust 1, 2023Shi M, Fu P, Bonventre JV, McCracken KW.

(12) A single-cell multiomic analysis of kidney organoid differentiationProceedings of the National Academy of Sciences of the United States of AmericaMay 16, 2023Yoshimura Y, Muto Y, Ledru N, Wu H, Omachi K, Miner JH, Humphreys BD.solution (Corning, 30-004-CI) for 4 d. On day 4, the medium was switched to 200 ng/mL FGF9 (R&D Systems, 273-F9-025) and 1 μg/mL heparin (Sigma-Aldrich, H4784) in the basal medium. On day 7, cells were dissociated into

(13) Protocol to analyze bioenergetics in single human induced-pluripotent-stem-cell-derived kidney organoids using Seahorse XF96STAR ProtocolsMarch 1, 2023Verónica Miguel, Katharina Charlotte Reimer, Anna Katharina Galyga, Jitske Jansen, Julia Möllmann, Luisa Meyer, Rebekka K. Schneider, Rafael Kramann,Thermo Fisher Scientific,15240062 CHIR99021,R&D Systems,4423/10 Recombinant Human FGF-9 Protein,R&D Systems,273-F9-025 Heparin sodium salt from porcine intestinal mucosa,Sigma-Aldrich,H4784 Recombinant Human BMP-7 Protein,R&D Systems

(14) The Grb2 splice variant, Grb3-3, is a negative regulator of RAS activationCommunications BiologySeptember 28, 2022Caroline Seiler, Amy K. Stainthorp, Sophie Ketchen, Christopher M. Jones, Kate Marks, Philip Quirke, John E. Ladburysuch as cancer. Methods Materials & reagents Recombinant human fibroblast growth factor 9 (FGF9; #273-F9-025), hepatocyte growth factor (HGF; 294-HG), transforming growth factor alpha (TGFα; 239-A) and epidermal

(15) Infecting kidney organoids with a cDNA reporter clone of SARS-CoV-2STAR protocolsSeptember 16, 2022Chung H, Bui-Marinos MP, Rahmani W, Corcoran JA, Chun J.#688001 16% aqueous Paraformaldehyde (PFA),EMS,Cat. #15710 Recombinant human FGF-9,R&D Systems,Cat. #273-F9-025 GlutaMax (100×),Thermo Fisher Scientific,Cat. #35050061 Phosphate buffered saline pH 7.4 (1× PBS),Thermo Fisher Scientific

(16) Heterogeneous Development of β-Cell Populations in Diabetes-Resistant and -Susceptible MiceDiabetesSeptember 1, 2022Gottmann P, Speckmann T, Stadion M, Zuljan E, Aga H, Sterr M, Büttner M, Santos PM, Jähnert M, Bornstein SR, et al.performed with MIN6 cells cultured in serum-free MIN6 medium with FGF-1 (4034-50; BioVision), FGF-9 (273-F9-025; R&D Systems), or BDNF (B-250; Alomone Labs) for 3 and 24 h. BrdU labeling (100 μmol/L, 2 h) was detected

(17) Human iPSC-derived renal organoids engineered to report oxidative stress can predict drug-induced toxicityiScienceMarch 18, 2022Lawrence ML, Elhendawi M, Morlock M, Liu W, Liu S, Palakkan A, Seidl LF, Hohenstein P, Sjögren AK, Davies JA.medium,Stemcell Technologies,05270 CHIR99201,Tocris,4423-10mg Recombinant human FGF9,R&D Systems,273-F9-025 Heparin,Stemcell Technologies,07980 Trypsin/ EDTA,Sigma-Aldrich,SLCB0546 Hydrogen peroxide to induce

(18) DevKidCC allows for robust classification and direct comparisons of kidney organoid datasetsGenome medicineFebruary 22, 2022Wilson SB, Howden SE, Vanslambrouck JM, Dorison A, Alquicira-Hernandez J, Powell JE, Little MH.On day 4, cells were switched to TeSR-E6 medium supplemented with 200ng/ml FGF9 (R&D Systems, cat# 273-F9-025) and 1 μg/ml Heparin (Sigma-Aldrich). On day 7, cells were dissociated with TrypLE, diluted five-fold

(19) Attenuation of SARS-CoV-2 infection by losartan in human kidney organoidsiScienceFebruary 18, 2022Rahmani W, Chung H, Sinha S, Bui-Marinos MP, Arora R, Jaffer A, Corcoran JA, Biernaskie J, Chun J.1 hr, the medium was changed to APEL2 + PFHM supplemented with FGF-9 (200 ng/mL, R&D Systems, Cat. #273-F9-025) and heparin (1 μg/mL, Millipore Sigma, Cat. #4784) for an additional 5 days, and then were maintained

(20) SARS-CoV-2 infects the human kidney and drives fibrosis in kidney organoidsCell stem cellFebruary 3, 2022Jansen J, Reimer KC, Nagai JS, Varghese FS, Overheul GJ, de Beer M, Roverts R, Daviran D, Fermin LAS, Willemsen B, et al.,Thermo Fisher,Cat#15240062 CHIR 99021,R&D Systems,Cat#4423/10 Recombinant Human FGF-9 Protein,R&D Systems,Cat#273-F9-025 Heparin sodium salt from porcine intestinal mucosa,Sigma-Aldrich,Cat#H4784 Recombinant Human BMP-7 Protein

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FGF-9 Protein

FGF-9 Protein

FGF-9 Protein

Recombinant Human FGF-9 Protein from R&D Systems, a Bio-Techne Brand

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R&D Systems, a Bio-Techne Brand

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Recombinant Human FGF-9 Protein from R&D Systems, a Bio-Techne Brand

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