TNF-alpha aa 80-235 Protein 410-MT-010 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemTNF-alpha aa 80-235 Protein
CompanyR&D Systems, a Bio-Techne Brand
Price $250.00Supplier Page View Company Product Page
Catalog Number410-MT-010
SpeciesMouse
Size10 ug (also 25 ug, 50 ug, 100 ug)
Molecule NameTNF-alpha
FormatLyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein
Purity>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
ApplicationsBioactivity
Conjugate/TagUnconjugated
NCBI Full Gene Nametumor necrosis factor
NCBI Gene AliasesTnlg1f, TNFalpha, DIF, Tnfsf1a, TNF-a, TNFA, TNFSF2, TNF-alpha, TNLG1F, Tnfa
Source/Expression SystemE. coli-derived Recombinant Mouse TNF-alpha (aa 80-235) Protein
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Sizes Available: 10 ug (also 25 ug, 50 ug, 100 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Phenotypic profiling of neutrophils in acute Clostridioides difficile infection identifies a TNF-induced activation signature associated with epithelial damageGut MicrobesDecember 31, 2026Alexander Huber, Ann Mathew, Faiza Khondokar, Divya Sharma, Anindita Mukherjee, Dolly K. Khona, Jucy Gabriel, Shinsmon Jose, Rajat Madanneutrophils Isolated bone marrow neutrophils were stimulated with or without recombinant murine TNF (50 ng/ml; R&D #410-MT-010) for 2 hours in RPMI-1640 (Thermo Fisher) + 10% FBS. TNF concentration used for these experiments was

(2) TFEB degradation is regulated by an IKK/β-TrCP2 phosphorylation-ubiquitination cascadeNature CommunicationsApril 1, 2026Yan Xiong, Jaiprakash Sharma, Meggie N. Young, Wen Xiong, Ali Jazayeri, Karl F. Poncha, Ma. Xenia G. Ilagan, Qing Wang, Bei Gao, Hui Zheng, et al.Gibco. Recombinant mouse IL-1β/IL-1F2 protein (Cat# 401-ML-005/CF), recombinant mouse TNFα protein (Cat# 410-MT-010), recombinant human TNFα protein (Cat# 210-TA-005) and recombinant human IL-1β/IL-1F2 protein (Cat#

(3) Weaning drives microbiome-mediated epigenetic regulation to shape immune memory in miceNature MicrobiologyMarch 19, 2026Li Yang, Robert C. Peery, Shirui Zhou, Xiaomin Chen, Leah M. Farmer, Fabiola Gutierrez, Stephanie Fowler, Lanjing Zhang, Julia M. Salamat, Karen Riggins, et al.single-agent treatment, organoids were treated for 48 h with 0.1 ng ml−1 IFNγ, 5 ng ml−1 TNF (R&D Systems, 410-MT-010), 2.5 μg ml−1 LPS (Thermo Fisher, 00-4976-03), 2 mM sodium butyrate (Sigma-Aldrich, 303410) or 0.08

(4) Islet-intrinsic sex differences in inflammatory signaling contribute to autoimmune diabetes susceptibilitybioRxivJanuary 16, 2026Kierstin L. Webster, Armando A. Puente, Jacob R. Enriquez, Soumyadeep Sarkar, Ernesto S. Nakayasu, Bobbie-Jo M. Webb-Robertson, Lisa M. Bramer, Kayla Figatner, Sarida Pratuangtham, Wenting Wu, et al.cocktail containing 25 ng/mL mouse IL-1β (R&D Systems; 401-ML-010), 50 ng/mL mouse TNF-α (R&D Systems; 410-MT-010), and 100 ng/mL mouse IFN-γ (R&D Systems; 485-MI-100) for 18 hours, or with 17-β estradiol (E2) (Millipore

(5) Enhanced Glioblastoma Immunotherapy via SMAC Mimetic Dose Escalation and TGFβ BlockadeNeuro-Oncology AdvancesDecember 10, 2025Kyle Malone, Melanie Dugas, Nathalie Earl, Tommy Alain, Robert G Korneluk, Eric LaCasse, Shawn T Beugcharacterize these dose effects on peripheral and tumor-associated immune cells. Methods Reagents TNF-α (410-MT-010), IL-1β (401-ml) and TGFβ (7666-MB-005) were purchased from R&D systems. IFNγ was purchased from R&D

(6) Inflammatory Signaling Induces Mitochondrial Dysfunction and Neuronal Death in Traumatic Brain Injury via Downregulation of OXPHOS GenesBiochemical geneticsDecember 1, 2025Dong H, Zhang H, Cai L, Ye Q, Wang H, Liu B, Zhang W, Li J.with 10% FBS and 1% pen/strep antibiotics. Cells were treated with 20 ng/mL mouse TNF-α (R&D Systems, 410-MT-010, Minneapolis, MN, USA) with or without 40 μM ZLN005 (Millipore Sigma, 573,596) for 48 h. ELISA Brain

(7) TCF25 serves as a nutrient sensor to orchestrate metabolic adaptation and cell death by enhancing lysosomal acidification under glucose starvationCell reportsSeptember 23, 2025Ren W, Jiang H, Song Q, Chen Y, Tang C, Wang F, Zhu J, Ren J, Zhao Y, He Y, et al.

(8) Adenosine A2a Receptor Stimulation Mitigates Periodontitis and Is Mitoprotective in Gingival Fibroblasts Promoting Cellular ResilienceCellsAugust 16, 2025Morandini AC, Dawson S, Paladines N, Adams N, Ramos-Junior ES.

(9) Identification of the pyroptosis, apoptosis, and necroptosis (PANoptosis) involved in osteogenic differentiation inhibition impaired by tumor necrosis factor-αEuropean journal of medical researchJuly 1, 2025Xia CL, Liang F, Li JX, Qu Y, Zhang W, Ou S, Yang Y, Qi Y, Xu CP.changed when the cell population reached 70%. The cells were stimulated with 10 µg/ml of TNF-α (Cat: 410-MT-010, R&D Systems) configured with dimethylsulfoxide (DMSO (Cat: D8371; Solarbio)), and different doses of

(10) Identification of the pyroptosis, apoptosis, and necroptosis (PANoptosis) involved in osteogenic differentiation inhibition impaired by tumor necrosis factor-αEur J Med ResJuly 1, 2025Chang-liang Xia, Fei Liang, Jia-xuan Li, Yudun Qu, Wei Zhang, Suanji Ou, Yang Yang, Yong Qi, Chang-peng Xuchanged when the cell population reached 70%. The cells were stimulated with 10 µg/ml of TNF-α (Cat: 410-MT-010, R&D Systems) configured with dimethylsulfoxide (DMSO (Cat: D8371; Solarbio)), and different doses of

(11) Modeling heterogeneous signaling dynamics of macrophages reveals principles of information transmission in stimulus responsesNat CommunJuly 1, 2025Xiaolu Guo, Adewunmi Adelaja, Apeksha Singh, Roy Wollman, Alexander Hoffmannwere then stimulated with specified concentrations (Table 1) of TNF (TNF-alpha (aa 80–235) protein, 410-MT-010, R&D Systems), CpG (synthetic CpG ODN 1668, tlrl-1668, Invivogen), Pam (Pam3CSK4, tlrl-pms, Invivogen),

(12) Repeated Low-Level Inflammatory Challenge Leads to Alterations in the TNF-CXCL10 Signalling Pathway in Mouse Cerebral Endothelial Cells In VitroJ NeurochemJune 16, 2025Megan Ritson, Dong Xia, Caroline Wheeler-Jones, Helen B. Stolp3 × 105 cells per well. Cells were treated with recombinant mouse TNF protein (R&D Systems; cat.no #410‐MT‐010) diluted in phosphate buffered saline (PBS; ThermoFisher; cat.no #10010023), with fresh complete medium

(13) Psychedelic control of neuroimmune interactions governing fearNatureMay 1, 2025Chung EN, Lee J, Polonio CM, Choi J, Akl CF, Kilian M, Weiß WM, Gunner G, Ye M, Heo TH, et al.were used to stimulate astrocytes, neurons and astrocyte–neuron cultures: 50 ng ml−1 TNF (R&D Systems, 410-MT-010); 100 ng ml−1 IL-1β (R&D Systems, 401-ML-005); 100 ng ml−1 IL-12 (Pepro-Tech, 210-12); and 200 ng ml−1

(14) Oral andrographolide loaded lipid nanocarriers alleviate stress behaviors and hippocampal damage in TNF alpha induced neuroinflammatory miceSci RepApril 8, 2025Sarawut Lapmanee, Natchanon Rimsueb, Phichaporn Bunwatcharaphansakun, Katawut Namdee, Prapimpun Wongchitrat, Sakkarin Bhubhanil, Nattapon Supkamonseni, Natthawut Charoenphon, Anjaree Inchan, Rattaporn Saenmuangchin, et al.inducing an inflammatory response. Recombinant mouse TNF-alpha (aa 80–235) protein (catalog number 410-MT-010, R&D Systems, Inc., Minnesota, USA) dissolved in sterile PBS and administered every 3 days for a total

(15) Tob negatively regulates NF-κB activation in breast cancer through its association with the TNF receptor complexCancer Gene TherapyApril 1, 2025Miho Tokumasu, Atsuko Sato, Taku Ito-Kureha, Mizuki Yamamoto, Nao Ohmine, Kentaro Semba, Jun-ichiro Inoue, Tadashi Yamamotocell stimulation; human TNF-α (210-TA-020, R and D systems, Minneapolis, MN, USA), and mouse TNF-α (410-MT-010, R and D systems), human-IL-1β (200-01B, Peprotech, Rocky Hill, NJ, USA), mouse Flag-tagged TNF-α (ALX-522-009,

(16) CLEC16A in astrocytes promotes mitophagy and limits pathology in a multiple sclerosis mouse modelNature NeuroscienceMarch 3, 2025Atsushi Kadowaki, Michael A. Wheeler, Zhaorong Li, Brian M. Andersen, Hong-Gyun Lee, Tomer Illouz, Joon-Hyuk Lee, Alain Ndayisaba, Stephanie E. J. Zandee, Himanish Basu, et al.and collected onto plates for primary mouse astrocyte cultures. TNF/IL-1β treatment (50 ng ml−1 TNF (410-MT-010, R&D Systems) and 100 ng ml−1 IL-1β (401-ML-005, R&D Systems)) was performed with the cytokines diluted

(17) KIRA6 is an Effective and Versatile Mast Cell Inhibitor of IgE-mediated ActivationEuropean journal of immunologyFebruary 1, 2025Wunderle V, Wilhelm T, Boukeileh S, Goßen J, Margreiter MA, Sakurov R, Capellmann S, Schwoerer M, Ahmed N, Bronneberg G, et al.

(18) Droplet-based functional CRISPR screening of cell-cell interactions by SPEAC-seqNature protocolsFebruary 1, 2025Faust Akl C, Linnerbauer M, Li Z, Lee HG, Clark IC, Wheeler MA, Quintana FJ.tlrl-eblps)Recombinant IL-1β (R&D Systems, cat. no. 401-ML-005)Recombinant TNF-alpha (R&D Systems, cat. no. 410-MT-010) Buffers and molecular biology Ex Taq DNA Polymerase, reaction buffer and dNTP mix (TaKaRa Bio Inc.,

(19) Nonenzymatic lysine D-lactylation induced by glyoxalase II substrate SLG dampens inflammatory immune responsesCell researchFebruary 1, 2025Zhao Q, Wang Q, Yao Q, Yang Z, Li W, Cheng X, Wen Y, Chen R, Xu J, Wang X, et al.

(20) Role of CXCL10 released from osteocytes in response to TNF-α stimulation on osteoclastsScientific reportsJanuary 24, 2025Miura M, Kitaura H, Ohori F, Narita K, Ren J, Noguchi T, Marahleh A, Ma J, Lin A, Fan Z, et al.

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Recombinant Mouse TNF-alpha (aa 80-235) Protein

TNF-alpha aa 80-235 Protein

TNF-alpha aa 80-235 Protein

TNF-alpha aa 80-235 Protein from R&D Systems, a Bio-Techne Brand

Description

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (398)

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TNF-alpha aa 80-235 Protein from R&D Systems, a Bio-Techne Brand

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