Fig 1: BMPR1ΔK-injected phenotypes after a 12h pulse of BMP4 starting at 1q or 4q stage. A Graphical representation of asymmetries present in BMPR1ΔK-injected, BMP pulse animals. Percentages are rounded to the nearest whole number. B-E BMPR1ΔK-injected, ventral view. F-G uninjected, ventral view. B, D, F 1q BMP 12h pulse. C, E, G 4q BMP 12h pulse B BMPR1ΔK-injected + 1q BMP4 animal with elongated foregut (arrow). C BMPR1ΔK-injected + 4q BMP4 animal with merged hemiganglia (arrowhead). D BMPR1ΔK-injected + 1q BMP4 animal with reduced right foregut, mesoderm and trunk muscles. E BMPR1ΔK-injected + 4q BMP4 animal with reduced left mesoderm and trunk muscles. Nuclei: white or cyan; muscles: yellow; stomodeum: asterisk; scale bar: 50 µm
Fig 2: Nuclear pSMAD1/5/8 levels approximately 10 h after BMPR1ΔK injection. A Boxplot showing the varying effect of BMPR1ΔK injection on the amount of nuclear pSMAD1/5/8 (measured as the relative brightness of labeling with an anti-pSMAD1/5/8 antibody in the nucleus); uninjected BMP-treated animals had significantly more nuclear pSMAD1/5/8 than animals without added BMP, while BMPR1ΔK-injected, BMP-treated animals have significantly less nuclear pSMAD1/5/8 than all treatments except seawater controls. Letters (a, b, c) indicate significance groups; white dots indicate individual cells. B Uninjected embryo in ASW, Arrows: cyan: higher nuclear pSMAD1/5/8; white: lower/no nuclear pSMAD1/5/8. C BMPR1ΔK-injected embryo in ASW. D Uninjected embryo with 1 h BMP4 pulse. E BMPR1ΔK-injected embryo with 1 h BMP4 pulse. Cyan: pSMAD1/5/8; Magenta: Phalloidin. Scale bar: 50 µm
Supplier Page from R&D Systems, a Bio-Techne Brand for Recombinant Zebrafish BMP-4 Protein