Fig 1: Effects of Wnt inhibition on migration of corneal epithelial cells.Confluent cultures mock- and SULF2 shRNA transduced THCE cells were wounded and treated with Wnt inhibitors (cocktail of DKK1/sFRP-1) or medium alone. Gaps were quantified at different times and reported as a percent of the remaining gap. Data are expressed as mean + SEM, n = 3 replicate wells per group. Statistical differences were calculated by the Student t-text. * denotes p<0.05, ** denotes p<0.01 for comparison of cells with mock knockdown (cnt shRNA) vs. SULF2 knockdown cells without Wnt inhibitors (SULF2 shRNA1), as well as with added Wnt inhibitors (SULF2 shRNA1+inhibitors). The statistical significance of the other comparisons is given in Results .