Fig 1: Effect of APN injection in OB on InsR protein content and insulin signaling components in OB homogenates. Photomicrograph shows the trajectory of the injection into OB (a). Representative images of immunoblotting show an increased in AMPK phosphorylated at Thr172 residue, a slight decrease of InsR content, a pronounced decrease of InsR phosphorylation, and slight reduction of phosphorylated Akt in OB homogenates 72 h after APN injection (b), respectively; however, none of the analyzed proteins had significative changes (c). Scale bar = 500 μm.