Recombinant Mouse M-CSF Protein 416-ML-010 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemRecombinant Mouse M-CSF Protein
CompanyR&D Systems, a Bio-Techne Brand
Price $292.00Supplier Page View Company Product Page
Catalog Number416-ML-010
Size10 ug (also 50 ug, 500 ug)
FormatLyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein
Purity>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
ApplicationsBioactivity
Conjugate/TagUnconjugated
SpeciesMouse
Molecule NameM-CSF
Molecular WeightObserved: 29 kDa, reducing conditions
Theoretical: 26 kDa (monomer)
Source/Expression SystemE. coli-derived Recombinant Mouse M-CSF Protein
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Full Gene Namecolony stimulating factor 1 (macrophage)
NCBI Gene AliasesBAP025, Csfm, MCSF, Mhdabap25, PG-M-CSF, op

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Paroxetine as a Therapeutic Agent in Inflammatory Osteolysis: Mechanistic Insights and EfficacyDrug Design, Development and TherapyJanuary 27, 2026Junming Huang, Zhipeng Wang, Jun Liu, Jihong Zhang, Kui Deng, Shanhu Huang, Song Zhouosteolysis management. Materials and Methods Reagents Recombinant mouse RANKL (462-TEC-010) and M-CSF (416-ML-010) were obtained from Bio-Techne (Minneapolis, MN). Paroxetine (HY-122272), SC79 (HY-18749), 740 Y-P (HY-P0175),

(2) Nature-inspired IL-1 targeted therapy to treat chronic inflammatory diseasesMolecular TherapyDecember 3, 2025Yeon-Suk Yang, Mi-Jeong Kim, Sachin Chaugule, Emma Mayer, Ngoc DeSouza, Hong Ma, Jun Xie, Ki-Young Lee, Shaoguang Li, Ellen Gravallese, et al.Non-adherent cells were collected and cultured in the presence of M-CSF (20 ng/mL; R&D Systems, catalog no. 416-ML-010) for 24 h to obtain monocytes. Cells were transduced with rAAV9 carrying pCBA.EGFP, pIR.EGFP, Vec, pCBA.IL-1Ra,

(3) BPAG1 promotes osteoclastogenesis and postmenopausal osteoporosis via activating ERK signaling pathwayiScienceNovember 21, 2025Yufeng Zhang, Han Zheng, Li Yang, Yi Wang, Liang Zhangcells were treated for 6 days with complete α-MEM supplemented with 30 ng/mL M-CSF (R&D Systems, Cat# 416-ML-010) and 50 ng/mL RANKL (R&D Systems, Cat#462-TEC-010) to induce differentiation. The culture medium was

(4) CTRP6 as a negative regulator of anti-inflammatory M2 macrophage polarizationImmunometabolism (Cobham)October 27, 2025Jeevotham Senthil Kumar, Emma Kempton, Muhammad Zubair Mehboob, Dingbo Lin, Xia Lei(Gibco, Thermo Fisher Scientific, Waltham, MA, USA), 20 ng/mL macrophage colony-stimulating factor (416-ML-010, R&D Systems, Minneapolis, MN, USA), 1% penicillin-streptomycin (Gibco, Thermo Fisher Scientific, Waltham,

(5) Modular inflammation network discovery from large-scale phenotypic screening in genetically heterogeneous mouse brainsJournal of neuroinflammationSeptember 29, 2025Xiong M, Miosge LA, Correa-Ospina C, Yan CMY, Cripps T, Bauernfried S, Wang Y, Crow M, Morris LX, Andrews TD, et al.

(6) Metabolic Profiling Reveals a Glycolytic Shift and an IRG1/Itaconate/NF2L2 Axis Regulating Neurotoxic Oxidative Stress in Inflammatory MicrogliaJournal of NeurochemistrySeptember 5, 2025Pinelopi Engskog‐Vlachos, Mikael K. R. Engskog, Martin Skandik, Kathleen Grabert, Noah Moruzzi, Marie‐Kim St‐Pierre, Ahmed M. Osman, Theodora Sylaidi, Klas Blomgren, Per‐Olof Berggren, et al.#31331028) and 10% heat‐inactivated FBS, supplemented with 10 ng/mL recombinant mouse M‐CSF (R&D Systems 416‐ML‐010). Cells were plated in a T75 flask and grown at 37°C in 5% CO2. The culture media were replaced every

(7) Asperosaponin VI inhibition of DNMT alleviates GPX4 suppression-mediated osteoblast ferroptosis and diabetic osteoporosisJournal of advanced researchSeptember 1, 2025Wei F, Ruan B, Dong J, Yang B, Zhang G, Kelvin Yeung WK, Wang H, Cao W, Wang Y.

(8) ATG7 in innate immune cells is required for host defense against nontuberculous mycobacterial pulmonary infectionsNature communicationsJuly 29, 2025Jeon SM, Lee YJ, Lee SH, Kim SI, Lee B, Roh T, Kim YJ, Kim HJ, Kim IS, Whang J, et al.Antibiotic-Antimycotic (15240062, Gibco). BMDMs were differentiated for 5 days in the presence of M-CSF (416-ML-010, R&D Systems). HEK293T (ATCC, CRL-3216) cells were cultured in DMEM and maintained at 37 °C in a humidified

(9) Programmed cell death protein 1 (PD-1) blockade regulates skeletal remodeling in a sex- and age-dependent mannerJournal of Bone and Mineral ResearchJuly 28, 2025Gwenyth J. Joseph, Lawrence A. Vecchi III, Sasidhar Uppuganti, Jeremy F. Kane, Margaret Durdan, Paige Hill, Ashtyn G. McAdoo, Hidenori Tanaka, David Kell, Madeline B. Searcy, et al.cells per 1 mL media per well of a 48 well plate and supplemented with 30 ng/mL M-CSF (R&D Systems, 416ML010) and 100 ng/mL RANKL (R&D Systems, 462TR010). Cells were treated with either IgG at 10 μg/mL or α-PD-1

(10) Ursodeoxycholic acid alleviates aortic aneurysm and dissection through the intestinal farnesoid X receptor/ceramide synthase 2 axisCommun BiolJuly 5, 2025Zhaofeng Zhang, Linfeng Xie, Xinfan Lin, Jian He, Yuling Xie, Jiakang Li, Xinghui Zhuang, Lele Tang, Rumei Xie, Qingsong Wu, et al.penicillin/streptomycin, 1% Hepes, 1% sodium pyruvate plus 10 ng/ml recombinant macrophage colony-stimulating factor (416-ML-010, R&D Systems, Shanghai, China). Differentiated cells were refed on day 3. On day 7, differentiated BMDMs

(11) Injectable magnesium-bisphosphonate MOF-based bone adhesive prevents excessive fibrosis for osteoporotic fracture repairNature communicationsJuly 1, 2025Xiao T, Gong Z, Duan D, Yu H, Liu S, Jiang Y, Xing X, Wu Z, Wang L, Yang XB, et al.

(12) Osteotropic properties of mesenchymal stromal cells generated in Masquelet's induced membrane techniqueScientific reportsMay 26, 2025Suzuki M, Kamitakahara M, Kihara W, Xie C, Kato H, Fukawa Y, Hamagaki M, Aoki K, Ichihara S, Ishijima M, et al.

(13) Engineered Spirulina platensis for treating rheumatoid arthritis and restoring bone homeostasisNature communicationsMay 13, 2025Yang X, Rong K, Fu S, Yang Y, Liu S, Zhang C, Xu K, Zhang K, Zhu Y, Hao Y, et al.

(14) CD4 + T Cells Expressing Viral Proteins Induce HIV-Associated Endothelial Dysfunction and Hypertension Through Interleukin 1α–Mediated Increases in Endothelial NADPH Oxidase 1CirculationApril 22, 2025Taylor C. Kress, Candee T. Barris, Laszlo Kovacs, Beryl N. Khakina, Coleton R. Jordan, Thiago Bruder-Nascimento, David W. Stepp, Rodger MacArthur, Vijay S. Patel, Jie Chen, et al.was induced via 20 ng/mL of murine macrophage colony stimulating factor (M-MCSF, R&D systems, CAT: 416-ML-010) applied every other day for 6 days, after which the media was replaced, and cells used for aorta-immune

(15) CAR T cell-driven induction of iNOS in tumor-associated macrophages promotes CAR T cell resistance in B cell lymphomaResearch squareMarch 31, 2025Davila M, Lee SB, Kang YP, Boucher J, Mandula J, Roselli E, Chang D, Jimenez R, Kotani H, Reid K, et al.

(16) Secreted neurofilament light chain after neuronal damage induces myeloid cell activation and neuroinflammationCell reportsMarch 25, 2025Kahn OI, Dominguez SL, Glock C, Hayne M, Vito S, Sengupta Ghosh A, Adrian M, Burgess BL, Meilandt WJ, Friedman BA, et al.

(17) Crohn's Disease-associated variant in laccase domain containing 1 (LACC1) modulates T cell gene expression, metabolism and T cell functionNature communicationsMarch 15, 2025Li Y, Ascui G, Dicker M, Riffelmacher T, Chandra V, Schmiedel B, Chou TF, Vijayanand P, Kronenberg M.

(18) Gut microbe-derived betulinic acid alleviates sepsis-induced acute liver injury by inhibiting macrophage NLRP3 inflammasome in micemBioMarch 12, 2025Tang X, Zeng T, Deng W, Zhao W, Liu Y, Huang Q, Deng Y, Xie W, Huang W.

(19) Maximizing Insights, Minimizing Animal Testing: A Framework for Validating Multiparametric Single-Cell Cytokine Analysis PanelsEuropean journal of immunologyMarch 1, 2025Aleith J, Bergmann-Ewert W, Müller-Hilke B.

(20) Immunoresponsive gene 1 facilitates TLR4 agonist-induced augmentation of innate antimicrobial immunityJournal of leukocyte biologyFebruary 13, 2025McBride MA, Caja KR, Patil TK, Owen AM, Luan L, Bohannon JK, Hernandez A, Stothers CL, Trenary IA, Rahim M, et al.

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(1)

11/11/2022Excellent Recombinant Mouse M-CSF Recombinant Protein Lucy LiuWe used this M-CSF protein for mice bone marrow-derived macrophage cell (BMDM) stimulation. This protein works great.
(1)

2/18/2020In Vitro Bioactivity in Cell Culture Joanna PlastiraA good and basic growth factor for microglia cultures.

Recombinant Mouse M-CSF Protein

M-CSF Protein

M-CSF Protein

M-CSF Protein

Recombinant Mouse M-CSF Protein from R&D Systems, a Bio-Techne Brand

Description

R&D Systems, a Bio-Techne Brand

Citations (714)

Reviews

Recombinant Mouse M-CSF Protein from R&D Systems, a Bio-Techne Brand

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