Fig 1: TcdBUK1 lacks significant ability to interact with FZD2. The wells of an ELISA plate were coated with TcdBVPI or TcdBUK1 followed by treatment with CSPG4-EC-GFP (1 nM) (A), FZD2-Fc (4 nM) (B), or PVRL3 (100 nM) (C). The amount of plate-bound CSPG4, FZD2, or PVRL3 was detected using each of the respective antibodies. Results are representative of at least two independent experiments. Error bars represent the mean deviations of results from duplicate samples.
Fig 2: Overlay of homology model of FBD from TcdBUK1 with the crystal structure of TcdBVPI (PDB code: 6C0B) in two different views (A, side view; B, front view). The toxin and the cysteine-rich domain 2 (CRD2) from FZD2 are represented in brown and green, respectively. Key positive allosteric modulator (PAM) (silver) binding residues from the toxin (brown) and CRD2 (green) are shown as stick models. The FBD sequence from TcdBM68 is identical to that from TcdBVPI.
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