Fig 1: Identification of a putative B2_Mm2-derived enhancer for Dicer1.(A) DESeq2 normalized counts showing immune-stimulated expression of Dicer1 in primary murine BMDMs. Data shown for untreated (n=3) BMDMs and BMDMs stimulated with IFNG for 2 hr (n=3) or 4 hr (n=3). ***DESeq2 FDR adjusted p-value <0.001, *DESeq2 FDR adjusted p-value <0.05. Error bars designate SEM. (B) Genome browser screenshot (http://genome.ucsc.edu) of the Dicer1 locus (Chr12:104,681,897–104,757,421) showing CPM-normalized ChIP-seq tracks for primary murine BMDMs and immortalized macrophage line J774A.1. B2_Mm2.Dicer1 (Chr12:104,742,467–104,742,646) is highlighted in gray. Values on the right of each track correspond to signal maxima. Bottom inset shows B2_Mm2.Dicer1 with accompanying STAT1 (IFNG 2 h Piccolo et al.) ChIP-seq signal and conservation tracks for rat, rabbit, human, cow, and dog. CTCF track derived from Gualdrini et al., 2022. RAD21 track derived from Cuartero et al., 2018. (C) RT-qPCR of wild type untreated (gray, n=3) J774A.1 cells and J774A.1 cells stimulated with IFNG (blue, n=3), IFNA (green, n=3), IL-2 (orange, n=3), IL-4 (light blue, n=3), or IL-15 (purple, n=3) for 4 hr. Treatments are indicated by color. *p-value <0.05, Student’s paired two-tailed t-test. BMDM: Bone-marrow-derived macrophage. SEM: Standard error of mean. CPM: Counts per million.
Fig 2: Identification of a putative B2_Mm2-derived enhancer for Dicer1.(A) DESeq2 normalized counts showing immune-stimulated expression of Dicer1 in primary murine BMDMs. Data shown for untreated (n=3) BMDMs and BMDMs stimulated with IFNG for 2 hr (n=3) or 4 hr (n=3). ***DESeq2 FDR adjusted p-value <0.001, *DESeq2 FDR adjusted p-value <0.05. Error bars designate SEM. (B) Genome browser screenshot (http://genome.ucsc.edu) of the Dicer1 locus (Chr12:104,681,897–104,757,421) showing CPM-normalized ChIP-seq tracks for primary murine BMDMs and immortalized macrophage line J774A.1. B2_Mm2.Dicer1 (Chr12:104,742,467–104,742,646) is highlighted in gray. Values on the right of each track correspond to signal maxima. Bottom inset shows B2_Mm2.Dicer1 with accompanying STAT1 (IFNG 2 h Piccolo et al.) ChIP-seq signal and conservation tracks for rat, rabbit, human, cow, and dog. CTCF track derived from Gualdrini et al., 2022. RAD21 track derived from Cuartero et al., 2018. (C) RT-qPCR of wild type untreated (gray, n=3) J774A.1 cells and J774A.1 cells stimulated with IFNG (blue, n=3), IFNA (green, n=3), IL-2 (orange, n=3), IL-4 (light blue, n=3), or IL-15 (purple, n=3) for 4 hr. Treatments are indicated by color. *p-value <0.05, Student’s paired two-tailed t-test. BMDM: Bone-marrow-derived macrophage. SEM: Standard error of mean. CPM: Counts per million.
Supplier Page from R&D Systems, a Bio-Techne Brand for IFN-alpha 2/IFNA2 Protein
Available conjugates: Sizes Available: 100000 UN