Casein Kinase II (CK2) P6010S from New England Biolabs

Product Specs
ItemCasein Kinase II (CK2)
CompanyNew England Biolabs
Price $170.00 Inquire
Catalog NumberP6010S
Size10000 units
Molecule NameCasein Kinase II (CKII)
ApplicationsProteomics & Glycomics
Temperature-80 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Dephosphorylation at Ser548 regulates hemocyanin-derived antibacterial peptides and immune defense in Penaeus vannameiiScienceMarch 20, 2026Qian Feng, Maoshuai Fu, Xianliang Zhao, Yongzhen Zhao, Qingyun Liu, Jude Juventus Aweya, Yueling Zhangdephosphorylated PvHMC (1 mg) was incubated at 30°C for 6 h with human CK2 (HsCK2, New England Biolabs, P6010S) in a reaction containing 10× NEB buffer (50 mM Tris-HCl, 10 mM MgCl2, 0.1 mM EDTA, 2 mM DTT, 0.01%

(2) The R2TP complex stabilises E7 to drive human papillomavirus-mediated pathogenesis in cellular models of cervical cancerVirol JMarch 5, 2026Mahaiwon Shadang, Aruna Arumugam, Dhiraj Kumar Singh, Pankaj Keshari, Sandeep Mathur, Venkateswaran K. Iyer, Seema Singhal, Shyam S. Chauhan, Qulsum Akhter, Riyaz Ahmad Mirkinase assay Purified GST-E7 fusion proteins were phosphorylated by Casein kinase 2 (New England Biolabs, Cat. P6010S) in the presence of 1X NEBuffer (50mM Tris-HCl, 10mM MgCl2, 0.1 mM EDTA, 2mM DTT, 0.01% Brij 35) and

(3) Enantioselective OTUD7B fragment discovery through chemoproteomics screening and high-throughput optimisationCommun ChemJanuary 15, 2025Aini Vuorinen, Cassandra R. Kennedy, Katherine A. McPhie, William McCarthy, Jonathan Pettinger, J. Mark Skehel, David House, Jacob T. Bush, Katrin Rittinger0.5 mM TCEP and 5% glycerol. OTUD5 was activated by treatment with CK2 kinase (New England BioLabs; P6010S) at 30 °C, 600 rpm for 4 h, and the reaction was quenched with 20 mM EDTA, as previously described43. 1

(4) MOTS-c modulates skeletal muscle function by directly binding and activating CK2iScienceNovember 15, 2024Hiroshi Kumagai, Su-Jeong Kim, Brendan Miller, Hirofumi Zempo, Kumpei Tanisawa, Toshiharu Natsume, Shin Hyung Lee, Junxiang Wan, Naphada Leelaprachakul, Michi Emma Kumagai, et al.and recombinant proteins" ,, WT MOTS-c,GenScript,– K14Q MOTS-c,GenScript,– Casein Kinase II,NEB,P6010S CK2α,GenScript,– CK2β,IBL,IBATGP4047 CK2 substrate peptide,Millipore Sigma,12–300 Insulin,Sigma-Aldrich,11070-73-8 ATP,NEB,P0756S Dexamethasone,Millipore Sigma

(5) PP2A licenses the FANCD2/FANCI complex for chromosome loadingCell ReportsNovember 12, 2024Di Yang, Fengxiang Bai, David Lopez Martinez, Hannan Xu, Ai Johjima-Murata, Lily Jiaqi Cao, Martin A. CohnMitomycin C from Streptomyces caespitosus,Sigma-Aldrich,Cat# M4287 Benzonase,Sigma-Aldrich,Cat# E1014 CK2,NEB,Cat# P6010S Lambda protein phosphatase,NEB,Cat# P0753S CX-4549,Enzo Life Sciences,Cat# ENZ-CHM151-0001 Propidium iodide

(6) The effect of phosphorylation efficiency on the oncogenic properties of the protein E7 from high-risk HPVVirus ResearchOctober 1, 2024Madison Malone, Ava Maeyama, Naomi Ogden, Kayla N. Perry, Andrew Kramer, Caleb Bates, Camryn Marble, Ryan Orlando, Amy Rausch, Caleb Smeraldi, et al.B6022SVIAL). 2.4 Phosphorylation efficiency assay CK2 was purchased commercially through New England Biolabs (P6010S), 500,000 units/mL. Each batch of CK2 was tested against E7(1–51) and compared with previously quantitated

(7) DYRK1A signalling synchronizes the mitochondrial import pathways for metabolic rewiringNature CommunicationsJune 20, 2024Adinarayana Marada, Corvin Walter, Tamara Suhm, Sahana Shankar, Arpita Nandy, Tilman Brummer, Ines Dhaouadi, F.-Nora Vögtle, Chris MeisingerTris/HCl, pH 7.2) supplemented with 5 mM ATP and 1 μl of the respective protein kinase (CK2α2β2, NEB, Cat#P6010S; CK2α1, ThermoFisher Scientific, Cat#PV3248; CK2α2, ThermoFisher Scientific, Cat#PV3624; DYRK1A, ThermoFisher Scientific

(8) Site-specific phosphorylation of ZYG-1 regulates ZYG-1 stability and centrosome numberiScienceDecember 15, 2023Jeffrey C. Medley, Rachel N. Yim, Joseph DiPanni, Brandon Sebou, Blake Shaffou, Evan Cramer, Colin Wu, Megan Kabara, Mi Hye Songnitrogen and stored at −80°C until use. In vitro kinase assays Recombinant human Casein Kinase 2 (NEB, P6010S) and Protein Kinase A (NEB, P6000S) were purchased from New England Biolabs. Recombinant GST::ZYG-1K41M

(9) FMRP phosphorylation modulates neuronal translation through YTHDF1Molecular cellDecember 7, 2023Zou Z, Wei J, Chen Y, Kang Y, Shi H, Yang F, Shi Z, Chen S, Zhou Y, Sepich-Poore C, et al.CK2 The phosphorylation of recombinant FMRP with CK2 was performed with commercial CK2 assay (NEB, P6010S) at 30 °C for one hour. The resulting phosphorylated FMRP was verified via western blotting. Tethering

(10) Inhibition of CK2/ING4 Pathway Facilitates Non‐Small Cell Lung Cancer ImmunotherapyAdvanced ScienceOctober 23, 2023Qian Gou, Huiqing Chen, Mingjun Chen, Juanjuan Shi, Jianhua Jin, Qian Liu, Yongzhong Houby glutathione‐conjugated sepharose beads. Active CK2 protein was obtained from New England Biolabs (P6010S). 100 units active CK2, GST‐ING4 or GST‐S150A (10 ng) were added to the reaction buffer (50 mM Tris‐HCl,

(11) Genome-wide kinase-MAM interactome screening reveals the role of CK2A1 in MAM Ca 2+ dynamics linked to DEE66Proceedings of the National Academy of SciencesAugust 8, 2023Truong Thi My Nhung, Nguyen Phuoc Long, Tran Diem Nghi, Yeongjun Suh, Nguyen Hoang Anh, Cheol Woon Jung, Hong Minh Triet, Minkyo Jung, Youngsik Woo, Jinyeong Yoo, et al.glutathione–Sepharose affinity chromatography. Purified proteins were incubated with recombinant CK2 from NEB (Cat#P6010S) in a 1X NEBuffer™ containing [γ-32P] ATP at 37 °C for 1 h. Reactions were terminated using 5× SDS sample

(12) The CHARGE syndrome-associated protein FAM172A controls AGO2 nuclear importLife science allianceAugust 1, 2023Sallis S, Bérubé-Simard FA, Grondin B, Leduc E, Azouz F, Bélanger C, Pilon N.Sino Biological (#11225-H07B #11079-H07B, respectively). Recombinant CK2 was purchased from New-England Biolabs (P6010S) and used to phosphorylate recombinant FAM172A (with MBP tag released by factor Xa-mediated cleavage)

(13) Site-Specific Phosphorylation of ZYG-1 Regulates ZYG-1 Stability and Centrosome NumberbioRxivJune 3, 2023Jeffrey C. Medley, Nahyun Yim, Joseph DiPanni, Brandon Sebou, Blake Shaffou, Evan Cramer, Colin Wu, Megan Kabara, Mi Hye Songnitrogen and stored at −80°C until use. In vitro Kinase Assays Recombinant human Casein Kinase 2 (NEB, P6010S) and Protein Kinase A (NEB, P6000S) were purchased from New England Biolabs. Recombinant GST::ZYG-1K41M

(14) Microtubule-associated proteins MAP7 and MAP7D1 promote DNA double-strand break repair in the G1 cell cycle phaseiScienceMarch 17, 2023Dullovi A, Ozgencil M, Rajvee V, Tse WY, Cutillas PR, Martin SA, Hořejší Z.33069-62-4 Phosphatase inhibitor,Sigma,Cat# 4906845001 Protease inhibitor,Sigma,Cat# 11836170001 CK2,NEB,Cat#P6010S ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, PLA duolink

(15) Phosphorylation of IGFBP-3 by Casein Kinase 2 Blocks Its Interaction with Hyaluronan, Enabling HA-CD44 Signaling Leading to Increased NSCLC Cell Survival and Cisplatin ResistanceCellsJanuary 25, 2023Coleman KL, Chiaramonti M, Haddad B, Ranzenberger R, Henning H, Al Khashali H, Ray R, Darweesh B, Guthrie J, Heyl D, et al.Nonglycosylated recombinant IGFBP-3 (MBS142177) was from MyBioSource (San Diego, CA, USA). Casein Kinase II (CK2, P6010S) was purchased from New England Biolabs (Ipswich, MA, USA). The caspase 3 (cleaved) colorimetric In-Cell

(16) CK2-Mediated Phosphorylation Upregulates the Stability of USP13 and Promotes Ovarian Cancer Cell ProliferationCancersDecember 29, 2022Kwon J, Zhang J, Mok B, Han C.was confirmed in SDS-gel running, followed by Coomassie brilliant blue staining. Active CK2 complex (P6010S), kinase buffer (B6022), and ATP (P0756S) were purchased from NEB (New England Biolabs, Ipswich, MA).

(17) A proteomic screen of Ty1 integrase partners identifies the protein kinase CK2 as a regulator of Ty1 retrotranspositionMobile DNANovember 18, 2022Barkova A, Adhya I, Conesa C, Asif-Laidin A, Bonnet A, Rabut E, Chagneau C, Lesage P, Acker J.Elmer) with recombinant proteins (200–500 ng) and yeast purified CK2 or recombinant human CK2 (NEB, P6010S). Proteins were resolved by SDS-PAGE and the gels were exposed to autoradiography or stained with Coomassie

(18) Phosphorylation and Stabilization of PD-L1 by CK2 Suppresses Dendritic Cell FunctionCancer researchJune 6, 2022Zhao X, Wei Y, Chu YY, Li Y, Hsu JM, Jiang Z, Liu C, Hsu JL, Chang WC, Yang R, et al.from E. coli (BL21). Purified recombinant proteins were incubated with 500 units of active CK2 kinase (P6010S, New England Biolabs) in kinase buffer containing 50 mM Tris-HCl at pH 7.6, 10 mM MgCl2, 2 mM DTT and

(19) Nutrient-responsive O-GlcNAcylation dynamically modulates the secretion of glycan-binding protein galectin 3The Journal of biological chemistryMarch 1, 2022Mathew MP, Abramowitz LK, Donaldson JG, Hanover JA.UDP-GlcNAz from the reaction mixture. A positive control was prepared by substituting casein kinase II (NEB, P6010S) for galectin 3 in the reaction mixture. The OGT assay mixtures were incubated for ∼90 min in a 37 °C

(20) Placental Mammals Acquired Functional Sequences in NRK for Regulating the CK2–PTEN–AKT Pathway and Placental Cell ProliferationMolecular Biology and EvolutionFebruary 3, 2022Beni Lestari, Satomi Naito, Akinori Endo, Hidenori Nishihara, Akira Kato, Erika Watanabe, Kimitoshi Denda, Masayuki Komada, Toshiaki FukushimaImageQuant LAS 4000 mini-imager. In Vitro CK2 Kinase Assay A purified CK2 complex was purchased (#P6010S, New England BioLabs, Beverly, MA). GST-mNRK565–868, GST-mNRK565–831, and GST-PTEN190–403 fusion proteins

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Casein Kinase II (CK2)

Casein Kinase II (CK2) from New England Biolabs

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New England Biolabs

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Casein Kinase II (CK2) from New England Biolabs

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