5-methyl-dCTP N0356S from New England Biolabs

Product Specs
Item5-methyl-dCTP
CompanyNew England Biolabs
Price $79.00 Inquire
Catalog NumberN0356S
Size1 µmol
Molecule Namedm5CTP
ApplicationsDNA Analysis
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Deaminase-Assisted Sequencing for the Identification of 5-glyceryl-methylcytosineBIO-PROTOCOLFebruary 20, 2026Bao-Dan He, Fan-Chen Wang, Jian-Huang Xue(Thermo Fisher Scientific, catalog number: FD1794) 5. 5-methyl-dCTP (2.5 mM) (NEB, catalog number: N0356S) 6. dATP (100 mM) (Takara, catalog number: 4026) 7. dTTP (100 mM) (Takara, catalog number: 4029) 8.

(2) The specificity landscape of WRKY transcription factors reveals the bidirectional influence of non-CG methylationNucleic Acids ResearchNovember 20, 2025Nana Ma, Dingkun Jiang, Tian Li, Lin Luo, Hao Chen, Xinfeng Zhang, Zongkai Mu, Shuyan Chen, Piaojuan Chen, Qing Liu, et al.between cycles were performed using the Taq DNA polymerase (Vazyme, P101) and with 5-methyl-dCTP (NEB, N0356S). DAP-seq-based assays Similar to as previously described [11], genomic DNA (gDNA) was extracted from

(3) Distinct Phage‐Encoded Enzymes for Substitution of Deoxythymidine by Deoxyuridine in Phage GenomesAdvanced ScienceSeptember 26, 2025Yating Li, Jason Tan, Yanqin Tu, Jingnan Wu, Zaifang Zhang, Yifeng Wei, Xinan Jiao, Yan Zhouwas purchased from Sangon Biotech (Shanghai, China). Amylose Resin (E8021V‐10 mL) and 5‐methyl‐dCTP (N0356S), BamHI‐HF (R3136V), HindIII‐HF (R3104V), EcoRI‐HF (R3101V), NotI‐HF (R3189V), and EnGen Lba Cas12a

(4) A DNA alteration and methylation co-detection method for clinical purposeEMBO Molecular MedicineJune 4, 2025Jiyan Yu, Chunhe Yang, Xintao Zhu, Zhankun Wang, Boping Xu, Ye Cai, Jingbo Zhao, Ruijian Guo, Wuzhou Yuan, Jianqing Wang, et al.cycle for gDNA and three cycles for cfDNA. Four modified dCTP analogs, including 5-methyl-dCTP (NEB, N0356S), 5-hydroxymethyl-dCTP (TriLink, N-2016), 5-carboxy-dCTP (TriLink, N-2063), and 5-propynyl-dCTP (TriLink,

(5) Methyl-CODEC enables simultaneous methylation and duplex sequencingNucleic Acids ResJune 4, 2025Ruolin Liu, Farzaneh Darbeheshti, Laurel Walsh, Rachel Li, Jin H Bae, Hayet Radia Zeggar, Azeet Narayan, Kan Xiong, G Mike Makrigiorgos, Viktor A Adalsteinssonevenly. We used four different conversion-resistant dCTPs, including 5-methyl-dCTP (NEB, catalog no. N0356S), 5-carboxy-dCTP (TriLink, catalog no. N-2063), 5-hydroxymethyl-dCTP (Jena Bioscience, catalog no. NU-932S),

(6) Rapid genome-wide profiling of DNA methylation and genetic variation using guide positioning sequencing (GPS)Frontiers in Cell and Developmental BiologySeptember 24, 2024Baolong Zhang, Wei Li, Jin Li, Yan Li, Huaibing Luo, Yanping Xi, Shihua Dong, Feizhen Wu, Wenqiang Yu4027Q)- dCTP (TaKaRa, cat. no. 4028Q)- dTTP (TaKaRa, cat. no. 4029Q)- 5-methyl-dCTP (NEB, cat. no. N0356S)- ATP (Thermo Scientific, cat. no. R0441)- NEBNext End Repair Enzyme Mix (NEB, cat. no. E6050L)- T4 DNA Polymerase

(7) TIME-seq reduces time and cost of DNA methylation measurement for epigenetic clock constructionNature AgingJanuary 10, 2024Patrick T. Griffin, Alice E. Kane, Alexandre Trapp, Jien Li, Matthew Arnold, Jesse R. Poganik, Ryan J. Conway, Maeve S. McNamara, Margarita V. Meer, Noah Hoffman, et al.Biolabs buffer 2, 5 μl of 5 mM deoxynucleotide triphosphate containing 5-methyl-dCTP (catalog number N0356S, New England Biolabs) instead of dCTP, and 2 μl of Klenow Fragment (3′ → 5′ exo-) (catalog number M0212L,

(8) Single-cell bisulfite-free 5mC and 5hmC sequencing with high sensitivity and scalabilityProceedings of the National Academy of SciencesDecember 5, 2023Yunlong Cao, Yali Bai, Tianjiao Yuan, Liyang Song, Yu Fan, Liuhao Ren, Weiliang Song, Jiahui Peng, Ran An, Qingqing Gu, et al.0.032 μL 100 mM dATP, 0.032 μL 100 mM dTTP, 0.032 μL 100 mM dGTP, 0.32 μL 10 mM 5-methyl-dCTP (NEB N0356S), 0.16 μL Q5 (NEB M0491S), 0.123 μL 120 ng/μL sonicated carrier lambda DNA] and incubated at 65 °C for

(9) Pronounced sequence specificity of the TET enzyme catalytic domain guides its cellular functionScience AdvancesSeptember 9, 2022Mirunalini Ravichandran et al.deoxynucleotide triphosphate (dNTP) mixture containing 2′-deoxy-5-methylcytidine 5′-triphosphate (5mdCTP) (NEB, N0356S) instead of 2′-deoxycytidine 5′-triphosphate. To generate substrates only methylated at CG sites, unmethylated

(10) Single nucleus multi-omics identifies human cortical cell regulatory genome diversityCell GenomicsMarch 9, 2022Chongyuan Luo et al.II First-Strand Buffer, 5mM DTT, 0.1% Triton X-100, 2.5 mM MgCl2, 500 μM each of 5′-methyl-dCTP (NEB N0356S), dATP, dTTP and dGTP, 1.2 μM dT30VN_4 oligo-dT primer (5′-AAGCAGUGGUAUCAACGCAGAGUACUTTTTTUTTTTTUTTTTTUTTTTTUTTTTTVN-3′

(11) NicE-C efficiently reveals open chromatin-associated chromosome interactions at high resolutionGenome researchMarch 1, 2022Luo Z, Zhang R, Hu T, Zhu Y, Wu Y, Li W, Zhang Z, Yao X, Liang H, Song X.NU-835-BIO14-S) and 1.5 μL of 1 mM biotin-14-dCTP (Jena Bioscience NU-956-BIO14-S), 5-methyl-dCTP (NEB, N0356S), 40 μL 50% PEG8000 in 200 μL 1× NEBuffer2. The nuclei were incubated at 37°C for 25 min for open chromatin

(12) Pre-Existing Dengue Immunity Drives a DENV-Biased Plasmablast Response in ZIKV-Infected PatientVirusesDecember 29, 2018Bhaumik SK, Priyamvada L, Kauffman RC, Lai L, Natrajan MS, Cho A, Rouphael N, Suthar MS, Mulligan MJ, Wrammert J.from being truncated, the cloning PCR was modified using 50 µM 5-methyl-dCTP (NEB; Ipswich, MA, USA; N0356S) and 150 µM dCTP. The heavy chain variable domains, irrespective of the original isotype, were cloned

(13) Comparative characterization of the PvuRts1I family of restriction enzymes and their application in mapping genomic 5-hydroxymethylcytosineNucleic Acids ResearchNovember 1, 2011Hua Wang, Shengxi Guan, Aine Quimby, Devora Cohen-Karni, Sriharsa Pradhan, Geoffrey Wilson, Richard J. Roberts, Zhenyu Zhu, Yu Zhenggt genomic or pUC19 DNA by using dATP/dGTP/dTTP mixed with dhmCTP (Bioline #BIO-39046), dmCTP (NEB #N0356S) or dCTP, respectively. PCRs were carried out using Phusion polymerase (NEB #M0530). The DNA fragment

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5-methyl-dCTP

5-methyl-dCTP from New England Biolabs

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New England Biolabs

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5-methyl-dCTP from New England Biolabs

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