Mca-RPKPVE-Nval-WRK(Dnp)-NH2 Fluorogenic MMP Substrate ES002 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemMca-RPKPVE-Nval-WRK(Dnp)-NH2 Fluorogenic MMP Substrate
CompanyR&D Systems, a Bio-Techne Brand
Price $337.00Supplier Page View Company Product Page
Catalog NumberES002
Size1 mg
ApplicationsBioactivity, Binding Activity
Purity>95%, by HPLC
Molecular Weight1676 Da
SpeciesMulti-Species
Molecule NameProtease Substrates
StorageSamples are stable for up to twelve months from date of receipt at -20° C to -70° C. The substrate can be aliquoted and stored -20° C to -70° C in a manual defrost freezer for six months. Avoid repeated freeze-thaw cycles. Protect from exposure to direct light

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Secretory leukocyte protease inhibitor influences periarticular joint inflammation in Borrelia burgdorferi-infected miceElifeMay 20, 2025Qian Yu, Xiaotian Tang, Thomas Hart, Robert Homer, Alexia A Belperron, Linda K Bockenstedt, Aaron Ring, Akira Nakamura, Erol Fikrigwith the fluorescent substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH2 Fluorogenic MMP Substrate (R&D Systems, #ES002) and the absorbance was monitored at 405 nm using a fluorescent plate reader (Tecan). Flow cytometry

(2) Secretory leukocyte protease inhibitor influences periarticular joint inflammation in B. burgdorferi-infected micebioRxiv : the preprint server for biologyMarch 28, 2025Yu Q, Tang X, Hart T, Homer R, Belperron AA, Bockenstedt LK, Ring A, Nakamura A, Fikrig E.

(3) Exploring Alternative Zinc-Binding Groups in Histone Deacetylase (HDAC) Inhibitors Uncovers DS-103 as a Potent Ethylhydrazide-Based HDAC Inhibitor with Chemosensitizing PropertiesJournal of Medicinal ChemistryFebruary 27, 2025Daniel Stopper, Lukas Biermann, Paris R. Watson, Jingyu Li, Beate König, Matthew N. Gaynes, Lais Pessanha Pessanha de Carvalho, Jana Klose, Maria Hanl, Alexandra Hamacher, et al.substrates Mca-PLGL-(DapDnp)-AR-NH2 for MMP1, MMP2, MMP9 and MMP13 and Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (ES002, from R&D systems) were used. The buffer used contains 50 mM Tris, 1 mM CaCl2, 150 mM NaCl and 0.05%

(4) Infectious parvovirus B19 circulates in the blood coated with active host protease inhibitorsNature communicationsNovember 5, 2024Lee H, Assaraf R, Subramanian S, Goetschius D, Bieri J, DiNunno NM, Leisi R, Bator CM, Hafenstein SL, Ros C.control. To measure peptidase activity, quenched fluorogenic peptide substrates were used (R&D systems, ES002). Recombinant human serpinA3 (R&D systems, 1295-PI) or purified B19V were incubated with α-chymotrypsin

(5) Discovery of a new class of cell-penetrating peptides by novel phage display platformScientific ReportsJune 11, 2024Jinsha Liu, John Heddleston, Douglas Raymond Perkins, Jack Jia Hua Chen, Ahmadreza Ghanbarpour, Bill William Smith, Rebecca Miles, Eitaro Aihara, Sepideh Afsharcathepsin D (Cat. No. ES001, R&D Systems), cathepsin H (Cat. No. 05859, Chemimpex), and cathepsin S (Cat. No. ES002, R&D Systems). Five µl of 200 µM peptide substrate was incubated with 5 µl of lysosomal extraction in

(6) Antibody-guided proteases enable selective and catalytic degradation of challenging therapeutic targetsThe Journal of biological chemistryMay 1, 2023Romei MG, Leonard B, Kim I, Kim HS, Lazar GA.to the protein solution. Fifty microliter of 2.5 μM fluorogenic MMP3 peptide substrate (R&D Systems, ES002) or 50 μg/ml DQ-collagen-IV (Invitrogen, D12052) in cleavage buffer was combined with the desired concentration

(7) PRSS2 remodels the tumor microenvironment via repression of Tsp1 to stimulate tumor growth and progressionNature communicationsDecember 27, 2022Sui L, Wang S, Ganguly D, El Rayes TP, Askeland C, Børretzen A, Sim D, Halvorsen OJ, Knutsvik G, Arnes J, et al.

(8) Human Coxsackie- and adenovirus receptor is a putative target of neutrophil elastase-mediated sheddingMolecular Biology ReportsApril 1, 2022Leonie Herrmann, Louise Schelletter, Raimund Hoffrogge, Karsten Niehaus, Volker Rudolph, Martin Farractivity was tested with fluorescent substrate II from R&D systems (Minneapolis, MN, USA; Cat.-No. ES002). All other MMPs cleave fluorescent substrate IX from R&D Systems (Cat.-No. ES010). PAGE, colloidal

(9) A Genus-Wide Bioactivity Analysis of Daboia (Viperinae: Viperidae) Viper Venoms Reveals Widespread Variation in Haemotoxic PropertiesInternational journal of molecular sciencesDecember 15, 2021Op den Brouw B, Coimbra FCP, Casewell NR, Ali SA, Vonk FJ, Fry BG.Metalloprotease (SVMP) and Serine Protease (SVSP) Activity Fluorogenic Peptide Substrates Cat#ES001, Cat#ES002, and Cat#ES0011 (R&D systems, Minneapolis, MN, USA) were used to test for SVMP, SVMP/SVSP, and SVSP

(10) Pharmacological Characterisation of Pseudocerastes and Eristicophis Viper Venoms Reveal Anticancer (Melanoma) Properties and a Potentially Novel Mode of FibrinogenolysisInternational Journal of Molecular SciencesJune 27, 2021Bianca op den Brouw, Parviz Ghezellou, Nicholas R. Casewell, Syed Abid Ali, Behzad Fathinia, Bryan G. Fry, Mettine H.A. Bos, Maria P. Ikonomopoulou400 cycles or until activity ceased. The assay was repeated using Fluorogenic Peptide Substrate Cat#ES002 (R&D systems, Minneapolis, MN, USA) under identical test conditions. 3.2.3. Snake Venom Serine Protease

(11) Extensive Variation in the Activities of Pseudocerastes and Eristicophis Viper Venoms Suggests Divergent Envenoming Strategies Are Used for Prey CaptureToxins (Basel)February 2, 2021Bianca op den Brouw et al.10 µL phospholipid. Activity was measured by adding 70 µL quenched Fluorogenic Peptide Substrate Cat#ES002 (R and D systems, Minneapolis, Minnesota, USA) (10 µL substrate in 5mL enzyme buffer: 150 mM NaCl, 50

(12) Design and synthesis of selective and blood-brain barrier-permeable hydroxamate-based gelatinase inhibitorsBioorganic chemistryJanuary 1, 2020Bertran A, Khomiak D, Konopka A, Rejmak E, Bulska E, Seco J, Kaczmarek L, Tarragó T, Prades R.

(13) An Allosteric Anti-tryptase Antibody for the Treatment of Mast Cell-Mediated Severe AsthmaCellOctober 3, 2019Henry R. Maun et al.agitation. A fluorescence-quenched peptide substrate, Mca-Arg-Pro-Lys-Pro-Val-Glu-Nval-Trp-Arg-Lys(Dnp)-NH2 (ES002, R&D Systems), was diluted to 10.5 μM in enzymatic assay buffer and added to the plates. Final in-well

(14) Factor X activating Atractaspis snake venoms and the relative coagulotoxicity neutralising efficacy of African antivenomsToxicology LettersMay 15, 2018Brice Oulion, James S. Dobson, Christina N. Zdenek, Kevin Arbuckle, Callum Lister, Francisco C.P. Coimbra, Bianca op den Brouw, Jordan Debono, Aymeric Rogalski, Aude Violette, et al.substrate only. For testing on RDES substrates (Fluorogenic Peptide Substrate, R & D systems Cat#s ES001, ES002, ES005 AND ES0011, Minneapolis, Minnesota), 10ul of 0.01 µg/µl venom stock was plated in triplicate

(15) Inhibition of Human Kallikrein 5 Protease by Triterpenoids from Natural SourcesMolecules (Basel, Switzerland)October 27, 2017Matsubara Y, Matsumoto T, Koseki J, Kaneko A, Aiba S, Yamasaki K.

(16) Protease-Inhibitor Interaction Predictions: Lessons on the Complexity of Protein-Protein InteractionsMolecular & cellular proteomics : MCPJune 1, 2017Fortelny N, Butler GS, Overall CM, Pavlidis P.and kallikrein 5 (KLK7, Catalogue# 2624-S.E.-010) and its quenched fluorescent substrate (Catalogue# ES002) were from R&D Systems. Cleavage of quenched fluorescent substrates was measured using excitation/emission

(17) Matrix Metalloprotease 3 Activity Supports Hippocampal EPSP-to-Spike Plasticity Following Patterned Neuronal Activity via the Regulation of NMDAR Function and Calcium FluxMolecular NeurobiologyJanuary 1, 2017Patrycja Brzdąk, Jakub Włodarczyk, Jerzy W. Mozrzymas, Tomasz Wójtowiczmanufacturer’s instructions and tested for specific activity with a fluorogenic peptide substrate (ES002, R&D Systems, USA) and fluorescence plate reader (EnSpire Multimode Plate Reader, PerkinElmer, USA). Glutamate-Evoked

(18) Structural analysis of asparaginyl endopeptidase reveals the activation mechanism and a reversible intermediate maturation stageCell researchMarch 1, 2014Zhao L, Hua T, Crowley C, Ru H, Ni X, Shaw N, Jiao L, Ding W, Qu L, Hung LW, et al.substrates Z-LR-AMC (R&D Systems, Catalog #: ES008) and Mca-RPKPVENval-WRK(Dnp)-NH2 (R&D Systems, Catalog #: ES002), respectively. Human cystatin C and the activated cathepsins were mixed in the assay buffer for 1 min,

(19) Vaspin inhibits kallikrein 7 by serpin mechanismCellular and molecular life sciences : CMLSJuly 1, 2013Heiker JT, Klöting N, Kovacs P, Kuettner EB, Sträter N, Schultz S, Kern M, Stumvoll M, Blüher M, Beck-Sickinger AG.manufacturers’ protocols using the recommended fluorogenic peptide substrates ES011 (hK4, hK5) and ES002 (hK7) (all from R&D) with minor modifications. Recombinant hK4 and hK7 were activated by thermolysin

(20) Matrix Metalloproteinase-10 Effectively Reduces Infarct Size in Experimental Stroke by Enhancing Fibrinolysis via a Thrombin-Activatable Fibrinolysis Inhibitor–Mediated MechanismCirculationDecember 20, 2011J. Orbe et al.Activity Assay MMP-10 activity was measured with the use of a fluorogenic peptide for stromelysins (ES002, Fluorogenic Peptide Substrate II, R&D Systems). Fibrin Plate Assay In a first set of experiments,

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Mca-RPKPVE-Nval-WRK(Dnp)-NH2 Fluorogenic MMP Substrate from R&D Systems, a Bio-Techne Brand

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R&D Systems, a Bio-Techne Brand

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Mca-RPKPVE-Nval-WRK(Dnp)-NH2 Fluorogenic MMP Substrate from R&D Systems, a Bio-Techne Brand

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