Fig 1: Primary human endothelial cells express noncanonical inflammasome proteins but not IL-1β or NLRP3 inflammasome proteins. HCAECs and human primary macrophages were transfected with a complex of ultra-pure LPS (2 µg/ml) and lipofectamine (5 µl/ml) or mock-treated with lipofectamine (5 µl/ml) or stimulated with extracellular LPS (1 µg/ml) for 6 h and protein expression of (a) IL-1β and (b) NLRP3 was analyzed by Western blotting from cell lysates. Representative Western blots and loading are shown. HCAECs, HUVECs and human primary macrophages were stimulated with extracellular LPS for indicated times and mRNA expression of inflammasome pathway proteins (c)NLRP3, (d)IL1B, (e)IL18, (f)CASP4, and (g)CASP5 was analyzed by RT-qPCR. Statistics: Two-way ANOVA followed by Sidak´s multiple comparisons test. a) n = 8, b-g) n = 4