Lambda DNA N3011S from New England Biolabs

Product Specs
ItemLambda DNA
CompanyNew England Biolabs
Price $76.00 Inquire
Catalog NumberN3011S
Size250 µg
Source/Expression SystemIsolated from bacteriophage lambda (cI857ind 1 Sam 7)
Molecule NameDNA
ApplicationsDNA Manipulation
Nucleic Acid TypeLambda DNA
Size Range48.5 Kbp
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Cell cycle dynamics regulate H3K27 and H3K9 histone modifications in DrosophilaPLOS BiologyMarch 26, 2026Liyne Nogay, Ananthakrishnan Vijayakumar Maya, Lara Heckmann, Francesco Cardamone, Isabelle Grass, Aakriti Singh, Anna Frey, Laurin Ernst, Nicola Iovino, Anne-Kathrin ClassenNEBNext HighFidelity 2× PCR Master Mix with 1 pg of Tn5-tagmented lambda DNA (New England Biolabs, #N3011S) as a spike-in normalizer. The library indexing was performed using Illumina i5 and i7 [106] through

(2) In‑Situ ssDNA Isolation from dsDNA Sources as a Streamlined Pathway to DNA Origami Assembly and TestingbioRxivMarch 25, 2026Enrique O. Ruiz, Kayla Neyra, Diana M. Lopez, Ruo-Wen Chen, Deepta Paramasamy, Quincy Bizjak, Patrick D. Halley, Yin Wei, Marcos Sotomayor, Michael G. Poirier, et al.Lambda DNA, pUC19, and phiX174 single-stranded and supercoiled DNA were purchased from New England Biolabs (N3011S, N3041S, N3021S, N3023S). 1kb DNA ladder at 500 μg/mL (cat. #N3232S) was purchased from New England BioLabs

(3) Whole-genome profiling of age- and sex-associated DNA methylation signatures in human plasma cell-free DNACommunications MedicineNovember 28, 2025Wei Chen, Jinjin Xu, Guodan Zeng, Rijing Ou, Changlin Yang, Chuang Xu, Yeqin Wang, Xinxin Wang, Qiuyan Li, Chenhui Zhao, et al.(Zymo Research). To evaluate bisulfite conversion efficiency, 0.05 ng of lambda DNA (New England Biolabs, #N3011S) was added to each reaction as an unmethylated control before bisulfite treatment. Subsequently, WGBS

(4) CLAE: A High‐Fidelity Nanopore Sequencing Strategy for Read‐Level Viral Variant Detection and Environmental RNA Virus DiscoveryAdvanced ScienceNovember 1, 2025Hannah Yu, Sarah Golconda, Ga‐Eun Lee, Dantong Xue, Guillermo Domínguez‐Huerta, James M. Wainaina, Benjamin Bolduc, Shashanka Murthy, Shihyoung Kim, Seth Faith, et al.the manufacturer's protocol. Bacteriophage lambda DNA (cI857ind 1 Sam 7) was purchased from NEB (NEB N3011). The genomic DNA was fragmented using a g‐Tube (Fisher Scientific NC0380758) with the 20 Kb target

(5) Genome-wide profiling of unmodified DNA using methyltransferase-directed tagging and enrichmentCell Rep MethodsOctober 20, 2025Luca Tosti, Calum Mould, Imogen Gatehouse, Anthony C. Smith, Krystian Ubych, Valentina Miano, Peter W. Laird, Jack Kennefick, Robert K. NeelyQ33231 Novaseq 6000 - S4 (300 Cycle) Sequencing Kit,Illumina,cat#: 20028312 Lambda phage DNA,NEB,cat#: N3011L KAPA HyperPrep Library Prep,Roche,cat#: KK8504 8 microTube-50 AFA Fiber V2 Strips,Covaris,cat#: 520174 AMPure

(6) Cytosine methylase and hydroxymethylase activity in mammalian mitochondriaFront Cell Dev BiolOctober 15, 2025Lisa S. Shock, Prashant V. Thakkar, Jason M. Robinson, Shirley M. Taylorcat # 39649), and 5hmC antibody (Active Motif, cat # 39770). Lambda DNA (New England BioLabs, cat #N3011L) was sonicated using a Diagenode Bioruptor water bath sonicator at the highest pulse for 2 cycles of

(7) The Plasmodium CSP repeats have elastic properties with a critical role in sporozoite motilityThe EMBO JournalSeptember 22, 2025Amanda E Balaban, Sachie Kanatani, Jaba Mitra, Jason Gregory, Natasha Vartak, Ariadne Sinnis-Bourozikas, Fredrich Frischknecht, Taekjip Ha, Photini Sinnispairing with the DNA conjugated to the peptide. For fluorescence force experiments, 16 nM λ-DNA (NEB #N3011S) in 0.120 M NaCl was heated to 80 °C for 10 min followed by 5 min on ice. The Cy3-Cy5-peptide construct

(8) The Eukaryotic homology search complex distorts donor DNA structure to probe for homologybioRxivSeptember 3, 2025Mitchell V. Woodhouse, Jingyi Hu, Meiling Wu, Jin Qian, James T. Inman, Michelle D. Wang, J. Brooks Crickarda total length of 12,688 bp DNA center (77). The center segment was PCR amplified from λ-DNA (NEB, N3011S), then double digested with AvaI (NEB, R0152S) and BssSI-v2 (NEB, R0680S) to produce the unique overhangs

(9) DNA Dynamics in Dual Nanopore Tug-of-WarArXivAugust 28, 2025Zezhou Liu, Wangwei Dong, Thomas St-Denis, Matheus Azevedo Silva Pessôa, Sajad Shiekh, Preethi Ravikumar, Walter Reisnerin the SiN membrane at the channel tips using focused ion beam (FIB). DNA samples The λ-DNA (cat. N3011S) is commercially available from the New England Biolabs®and the T4 GT7 DNA (cat. 318–03971) is available

(10) Single-molecule tweezers decode hidden dimerization patterns of membrane proteins within lipid bilayersNat CommunAugust 9, 2025Victor W. Sadongo, Eojin Kim, Seoyoon Kim, W. C. Bhashini Wijesinghe, Taeseung Lee, Jeong-Mo Choi, Duyoung Minbiotins (2×biotin), were generated using polymerase chain reaction (PCR) with λ DNA template (NEB, N3011S) and synthesized primers (IDT). The primers used in the PCR were a forward primer (ACAGAAAGCCGCAGAGCA)

(11) Functional interplay between condensin I and topoisomerase Iiα in single-molecule DNA compactionNat CommunAugust 6, 2025Yuko Tsubota, Keishi Shintomi, Kazuhisa Kinoshita, Yuki Masahara-Negishi, Yuuki Aizawa, Masami Shima, Tatsuya Hirano, Tomoko Nishiyamawithdraw solutions. The channels were washed with ELB + +. Biotin-conjugation at both ends of λDNA (N3011, New England Biolabs) was performed as follows: 0.15 pmol λDNA and two biotinylated oligonucleotides,

(12) Mechanism of Rad51 filament formation by Rad52 and Rad55-Rad57 in homologous recombinationNat CommunJuly 21, 2025Jaigeeth Deveryshetty, Ayush Mistry, Sushil Pangeni, Mohamed Ghoneim, Monica Tokmina-Lukaszewska, Steven K. Gore, Jie Liu, Vikas Kaushik, Simrithaa Karunakaran, Angela Taddei, et al.biotins on the 3′ and 5′ ends of the same strand. Lambda DNA was purchased from New England Biolabs (N3011). This linear DNA comes with 12 nucleotides (nt) 5′ overhangs. Three short oligos to anneal to the sticky

(13) Kinetic analysis and engineering of thermostable Cas12a for nucleic acid detectionNucleic Acids ResJune 13, 2025Juan Pan, Megumu Mabuchi, Daniel W Kneller, Ryan T Fuchs, Jennifer L Curcuru, Esta Tamanaha, Nathan A Tanner, G Brett Robb, Ivan R Corrêa(#B0537S), WarmStart® RTx Reverse Transcriptase (#M0380L), dNTP solution mix (#N0447L), Lambda DNA (#N3011S), and Recombinant Albumin, Molecular Biology Grade (#B9200S) were all from New England Biolabs (Ipswich,

(14) FACT weakens the nucleosomal barrier to transcription and preserves its integrity by forming a hexasome-like intermediateMolecular CellJune 5, 2025Francesca Burgos-Bravo, Alexander B. Tong, Chen Li, César Díaz-Celis, Craig D. Kaplan, Gary LeRoy, Danny Reinberg, Carlos BustamanteMaxiprep,Zymo Research,Cat# D4202 Recombinant DNA,Recombinant DNA,Recombinant DNA Lambda DNA,NEB,Cat# N3011S Plasmid: pGEM-3z/12x601,This paper,N/A Plasmid: pGEM-3Z/5S,This paper,N/A Plasmid: pGEM-3Z/601,Addgene,Cat#

(15) H2A.Z deposition by the SWR complex is stimulated by polyadenine DNA sequences in nucleosomesPLoS BiolMay 12, 2025Cynthia Converso, Leonidas Pierrakeas, Lirong Chan, Shalvi Chowdhury, Emily de Onis, Vyacheslav I. Kuznetsov, John M. Denu, Ed Luk[70.5% w/v sucrose in 10 mM Tris-HCl (pH 7.8), 1 mM EDTA] and 1 μL 20 μg/μL lambda phage DNA (NEB, Cat.# N3011S). The samples were separated on a 6% polyacrylamide (0.5x TBE, 1.5 mM thickness) at 110 V for 2 hr using

(16) Chromatin landscape at cis-regulatory elements orchestrates cell fate decisions in early embryogenesisNat CommunMarch 27, 2025Francesco Cardamone, Annamaria Piva, Eva Löser, Bastian Eichenberger, Mari Carmen Romero-Mulero, Fides Zenk, Emily J. Shields, Nina Cabezas-Wallscheid, Roberto Bonasio, Guido Tiana, et al.NEBNext HighFidelity 2× PCR Master Mix with 1 pg of Tn5-tagmented lambda DNA (New England Biolabs, #N3011S) spike-in as normalizer supplemented in the NEBNext HighFidelity 2× PCR Master Mix (New England Biolabs,

(17) Longitudinal assessment of the prevalence of Fusobacterium necrophorum, Fusobacterium varium, and Salmonella enterica in the nasal cavity, ruminal fluid, and feces of finishing beef steers with and without liver abscessesFrontiers in MicrobiologyMarch 20, 2025Colten W. Dornbach, Paul R. Broadway, James E. Wells, Kallie D. Childress, Aubrey C. Thompson-Smith, Landon G. Canterbury, Nicole C. Burdick Sanchez, Jacque Mathieu, Cory Schwarz, Jenny Laverde Gomez, et al.μL ZymoBIOMICS™ Lysis Solution, 375 μL of DNA/RNA Shield™, and 1×106 copies of lambda phage genome (N3011S; New England Biolabs, Ipswich, MA). Following mechanical lysis with a Biospec Mini-Bead Beater-16 (BioSpec

(18) Probing the potential of rare earth elements in the development of new anticancer drugs: single molecule studiesBeilstein J NanotechnolFebruary 14, 2025Josiane A D Batista, Rayane M de Oliveira, Carlos H M Lima, Milton L Lana Júnior, Virgílio C Dos Anjos, Maria J V Bell, Márcio S Rocha7.4. The samples used for OT assays consist of biotin-labeled λ DNA molecules (New England Biolabs N3011S) tethered by the ends between a streptavidin-coated coverslip and a streptavidin-coated polystyrene

(19) Domainator, a flexible software suite for domain-based annotation and neighborhood analysis, identifies proteins involved in antiviral systemsNucleic Acids ResearchJanuary 11, 2025Sean R Johnson, Peter R Weigele, Alexey Fomenkov, Andrew Ge, Anna Vincze, James B Eaglesham, Richard J Roberts, Zhiyi Sungenomic DNA with Dam methylation (methylation at the N6 position of adenine within the GATC sequence) (N3011, NEB, MA) and λ genomic DNA free of Dam methylation (N3013, NEB, MA). All RE enzymes, DNA substrates,

(20) Single-cell mapping of regulatory DNA:Protein interactionsbioRxivJanuary 2, 2025Wei-Yu Chi, Sang-Ho Yoon, Levan Mekerishvili, Saravanan Ganesan, Catherine Potenski, Franco Izzo, Dan Landau, Ivan RaimondiSystem (Supplementary Fig. 1b). Deamination assay DNA substrates, including lambda phage DNA (NEB, N3011) or 5’ 6-FAM-labeled 30-mer dsDNA oligonucleotides (IDT, Supplementary Note) were used for testing nb-DddA

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Lambda DNA

Lambda DNA from New England Biolabs

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Lambda DNA from New England Biolabs

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