O-Glycosidase P0733L from New England Biolabs

Product Specs
ItemO-Glycosidase
CompanyNew England Biolabs
Price $604.00 Inquire
Catalog NumberP0733L
Molecule NameEndo-alpha-N-Acetylgalactosaminidase
Size10000000 units
ApplicationsProteomics & Glycomics
Temperature-20 C
TypeGlycosidases

Add to Compare List

New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Engineering Soluble Recombinant BCMA for Ide-Cel LabelingEur J ImmunolJuly 29, 2026Ly Phuong Vy Nguyen, Christelle Oblet, Alice Barbarin, Louis Waeckel, Farah Kotaich, Clara Petit, Marine Mabily, Claire Carrion, André Herbelin, Jean Marc Gombert, et al.PNGase F glycan cleavage kit (#A39245, Gibco). O‐glycan removal was performed using O‐glycosidase (#P0733, New England Biolabs) and α2‐3,6,8,9 neuraminidase A (#P0722, New England Biolabs). O‐ and N‐glycans

(2) A Quartet of Native Orai Channel Isoforms Orchestrates Graded NFAT Activation and TranscriptionbioRxivJuly 13, 2026Ahmed Emam Abdelnaby, Yin-Hu Wang, J. Cory Benson, Isagani Perez, Mark Shen, Maxwell McDermott, Miki Jishage, Amal T. Elhaw, Silvia Cruz-Rangel, Raphael Courjaret, et al.BCA assay. To perform deglycosylation either Protein Deglycosylation Mix II O-Glycosidase kit (NEB #P0733) or N-Glycosidase F (Millipore Sigma) were used. For the Protein Deglycosylation Mix II the manufacturer

(3) Transferrin receptor 1 binds human parvovirus B19 VP1u to facilitate entryNature CommunicationsJune 11, 2026Hyunwook Lee, Jan Bieri, Nicolas Ammann, Corinne Suter, Daniela Hunziker, Ajit K. Singh, Carol M. Bator, Susan L. Hafenstein, Carlos RosU; 50 mM sodium phosphate, pH 7.5; P0704, NEB), O-glycosidase (10 U; 50 mM sodium phosphate, pH 7.5; P0733, NEB), β1-4-galactosidase (10 U; 50 mM sodium acetate, pH 5.5, 5 mM CaCl₂; P0745, NEB), β-N-acetylglucosaminidase

(4) Multiple glycoforms of TrkA interact with N-cadherin during trigeminal ganglion neurodevelopmentJournal of Cell ScienceMay 1, 2026Caroline A. Halmi, Lisa A. Taneyhilltreatments, equal amounts of trigeminal ganglia lysate were used. PNGase F (NEB, P0704S), O-glycosidase (NEB, P0733S) and α2-3,6,8 neuraminidase (NEB, P0720S) were added to lysate per manufacturer's guidelines. Samples

(5) A hierarchical lectin-based multimodal workflow for spatial mapping of extracellular matrix glycans in fibrotic heartsAnalytical and Bioanalytical ChemistryApril 27, 2026Patcharaporn Boottanun, Chiaki Nagai-Okatani, Kunio Kawanishi, Masaki Baba, Tomofumi Nakatsukasa, Tomoko Ishizu, Kiyohiko Angata, Atsushi Kuno(catalog number: GK80040, New England BioLabs) and O-glycosidase 4000 units (total/slide) (catalog number: P0733, New England BioLabs) in 1× reaction buffer (50 mM sodium phosphate, pH 7.5) containing 0.5% NP-40 at

(6) Aberrant laminin signaling drives melanocyte dedifferentiation and unveils a tractable therapeutic target in vitiligoNature CommunicationsApril 17, 2026Fei Yang, Lingli Yang, Sylvia Lai, Masafumi Yokota, Yasutaka Kuroda, Takuo Yuki, Yoshito Takahashi, Tetsuya Sayo, Takeshi Namiki, Hiroyuki Goto, et al.were gently washed with PBS. Subsequently, cells were incubated with O-glycosidase (0.05–0.5 U/mL; #P0733S, New England Biolabs) in fresh Opti-MEM medium for another 2 h to remove the exposed oligosaccharides.

(7) Disrupted O-GalNAc glycosylation as a mechanism and biomarker of SLC35A2 -associated epilepsybioRxivMarch 4, 2026Robert G. Mealer, James J. Anderson, Sheridan L. Smith, Brianna M. Masters, Samuel H. Barth, Kaitlyn D. J. Huizar, Sahibjot Sran, Hyojung Yoon, Amanda Ringland, Savannah J. Muron, et al.performed at 37°C overnight following manufacturers protocols (NEB, Neuraminidase A #P0722L, O-Glycosidase #P0733L, PNGase F #P0704L, and α1–3,4 Fucosidase #P0769S; Chondroitinase ABC, Sigma #C2905–5UN). Following transfer

(8) STT3A-mediated FCN3 N-glycosylation promotes Treg cell activation to drive hepatocellular carcinoma progression via Wnt/β-cateninCell Oncol (Dordr)January 6, 2026Hongli Zhang, Peng Zhang, Tian Gong, Chengsheng Zhang, Zhijian Wang, Yong ZhaoPNGase F (#P0704S, New England BioLabs, Ipswich, MA, USA) for N-glycosylation removal or O-glycosidase (#P0733, New England BioLabs) for O-glycosylation removal following manufacturer’s instructions. WB analysis

(9) EatA mediated degradation of intestinal mucus is species-specific and driven by MUC2 structural featuresNature CommunicationsDecember 31, 2025Sergio Trillo-Muyo, Brendan Dolan, Frida Svensson, Tim J. Vickers, Liisa Arike, Maria-Jose García-Bonete, Jenny K. Gustafsson, Mathias I. Nielsen, Hans H. Wandall, James M. Fleckenstein, et al.(P0726, New England Biolabs), α2-3,6,8 Neuraminidase (P0720, New England Biolabs) and O-glycosidase (P0733, New England Biolabs) either as a single reaction or sequentially. All reactions were set up according

(10) β-Arrestin 1 Differentially Modulates cAMP and ERK Pathways Downstream of the FSH ReceptorCurr Issues Mol BiolDecember 16, 2025Sei Hyun Park, Munkhzaya Byambaragchaa, Ye Rin Yu, Jae Won Lee, Min-Jeong Kwak, Seung-Bin Yoon, Ji-Su Kim, Myung-Hwa Kang, Kwan-Sik MinFreeStyle CHO expression medium were purchased from Invitrogen Corporation (Carlsbad, CA, USA). PNGase F (P0704), O-Glycosidase (P0733), and α2-3,6,8,9 Neuraminidase A (P0722) were purchased from New England Biolabs (Ipswich, MA, USA).

(11) Conversion of an agonistic anti-TNFR2 biparatopic antibody into an antagonist by insertion of peptide linkers into the hinge regionJournal of Biological ChemistrySeptember 1, 2025Takuya Otsuki, Shigeyuki Matsumoto, Junso Fujita, Tomoko Miyata, Keiichi Namba, Ryo Kanada, Yasushi Okuno, Haruhiko Kamada, Hiroaki Ohno, Hiroki Akiba(1 mg), dialyzed to PBS after Protein A affinity chromatography, was mixed with 2 μl O-glycosidase (#P0733S, New England BioLabs), 2 μl α2-3,6,8 neuraminidase (#P0720S, New England BioLabs), 15 μl of 10 × GlycoBuffer

(12) METTL9 sustains vertebrate neural development primarily via non-catalytic functionsNature communicationsAugust 1, 2025Codino A, Spagnoletti L, Olobardi C, Cuomo A, Santos-Rosa H, Palomba M, Margaroli N, Girotto S, Scarpelli R, Luan SL, et al.(New England Biolabs, #P0703S), rPNGase F (New England Biolabs, #P0704S) or O-Glycosidase (New England Biolabs, #P0733S) and Neuromidase treatment, for 2 hrs at 37 °C. Additional reactions were prepared in parallel (as described

(13) Surface N- or O-linked glycans on bovine spermatozoa play minimal role in evading macrophage mediated phagocytosisFrontiers in Veterinary ScienceMarch 24, 2025Jatinder Singh Chera, Vikrant Gaur, Abhishek Kumar, Fanny Josan, Aditya Patel, Sonam Yadav, Seema Karanwal, Preeti Verma, Vivek Verma, Sushil Kumar, et al.units of α2-3,6,8,9 Neuraminidase A (P0722, New England Biolabs) and 60,000 units of O-glycosidase (P0733, New England Biolabs) for different time points (1, 2 and 4 h) at 37°C in a humidified incubator (NU-8600E,

(14) O-GalNAc glycans are enriched in neuronal tracts and regulate nodes of RanvierProceedings of the National Academy of SciencesMarch 4, 2025Maxence Noel, Suttipong Suttapitugsakul, Richard D. Cummings, Robert G. Mealerprotein lysate was treated with 5 μL NeuA (α2-3,6,8,9 NeuA, P0722L, NEB) or 5 μL of O-Glycosidase (P0733L, NEB) where indicated prior to denaturation/loading on sodium dodecyl-sulfate polyacrylamide gel electrophoresis

(15) The Shab family potassium channels are highly enriched at the presynaptic terminals of human neuronsThe Journal of biological chemistryMarch 1, 2025Benner O, Karr CH, Quintero-Gonzalez A, Tamkun MM, Chanda S.combined with 10x GlycoBuffer2, PNGase F (New England Biolabs; P0709), O-glycosidase (New England Biolabs; P0733), or both enzymes combined, in ratios according to the manufacturer’s protocol. In control conditions,

(16) Dual functional POGases from bacteria encompassing broader O-glycanase and adhesin activitiesNat CommunFebruary 25, 2025Linjiao Zhou, Uriel Ortega-Rodriguez, Matthew J. Flores, Yasuyuki Matsumoto, John Q. Bettinger, Wells W. Wu, Yaqin Zhang, Su-Ryun Kim, Thomas G. Biel, Jordan D. Pritts, et al.1-(4-MU), Core 2-(4-MU), Core 3-(4-MU), or α2,3sialylCore 1-(4-MU) for POGase AS and O-glycosidase (EngEF, P0733S, New England Biolabs, MA). Kinetic assays were carried out at 37 °C in 20 mM citrate/HCl, 50 mM NaCl,

(17) Imaging lipid rafts reveals the principle of ApoE4-induced Aβ upregulation in human neuronsiScienceFebruary 21, 2025Lee SI, Lim H, Kim NY, Yu J, Cho J, Lee H, Moon DW, Seo J.Recombinant human BMP-4,PeproTech,Cat#AF-120-05ET DPBS,Biowest,Cat#L0615-500 Streptavidin-conjugated QD-655,Invitrogen,Cat#Q10121MP Q-Glycosidase,New England Biolabs,Cat#P0733 PNGase F,Sigma-Aldrich,Cat#P7367 Cholesterol,Calbiochem,Cat#228111 Ammonium acetate,Daejung,Cat#2511-4405 Methyl-β-cyclodextrin,Sigma-Aldrich,Cat#C4555 Dynasore,Sigma-Aldrich,Cat#D7693 ,, Critical

(18) A Novel Missense Variant of BMPR1A in Juvenile Polyposis Syndrome: Assessment of Structural and Functional AlternationsHuman MutationFebruary 18, 2025Mengyuan Yang, Ziyan Tong, Zhijun Yuan, Bingjing Jiang, Yingxin Zhao, Dong Xu, Ying Yuantreated with N-glycosidase (N-Gly) F (PNGase F, P0704S) to remove N-glycans or O-glycosidase (O-Gly) (P0733S) and α2-3,6,8 neuraminidase (P0720S) to remove O-glycans, and these enzymes are all purchased from New England Biolabs

(19) C1GALT1C1 ‐Associated Mosaic Disorder of Glycosylation in a FemaleJ Inherit Metab DisFebruary 13, 2025Rajindra P. Aryal, Aditya Ramanujan, Camille Bucci, Christian Neckelmann, Jamie Heimburg-Molinaro, Sandra F. Cummings, Florian Erger, Bodo B. Beck, Laurie H. Seaver, Richard D. Cummingsmock treatment, 1 μL of α2‐3,6,8,9 NeuA (Cat#P0722S), a combination of NeuA and O‐glycosidase (Cat#P0733S), or PNGase‐F (Cat#P0708S) for 2 h at 37°C, following the manufacturer's protocol from NEB. Approximately

(20) The O-glycosyltransferase C1GALT1 promotes EWSR1::FLI1 expression and is a therapeutic target for Ewing sarcomaNature communicationsFebruary 2, 2025Banday S, Mishra AK, Rashid R, Ye T, Ali A, Li J, Yustein JT, Kelliher MA, Zhu LJ, Deibler SK, et al.(New England BioLabs, Cat# P0720S) in the presence or absence of 800 U O-glycosidase (New England BioLabs, Cat# P0733S) for 2 h at room temperature, and then incubated with PNA- or jacalin-conjugated agarose beads (Vector Laboratories

Be the First to Write a Review!

O-Glycosidase

O-Glycosidase from New England Biolabs

Description

New England Biolabs

Citations (73)

Reviews

O-Glycosidase from New England Biolabs

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map