Sandwich ELISA analysis of human adiponectin binding using Mouse anti Human adiponectin, Adn27 as a capture reagent and biotinylated Mouse anti Human adiponectin antibody, clone 5H7 as a detection reagent with recombinant human adiponectin (PHP196) as antigen for the generation of the standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). A plasma sample diluted 1:4 (green) is shown.
RECOMBINANT HUMAN ADIPONECTIN
Sandwich ELISA analysis of human adiponectin binding using Mouse anti Human adiponectin as a capture reagent and biotinylated Mouse anti Human adiponectin as a detection reagent with recombinant human adiponectin (PHP196) as antigen for the generation of the standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader . A plasma sample diluted 1:4 (green) is shown.
Sandwich ELISA analysis of human adiponectin binding using Mouse anti Human adiponectin (MCA4652) as a capture reagent and biotinylated Mouse anti Human adiponectin (MCA2695) as a detection reagent with recombinant human adiponectin (PHP196) as antigen for the generation of the standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). A plasma sample diluted 1:4 (green) is shown.
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