Human IL-1β, premium grade 130-093-899 from Miltenyi Biotec

(1) Overcoming claudin family homology: discovery of ARC101, a highly potent CLDN6-specific T-cell engager with a novel CD3 binder for ovarian adenocarcinomaMAbsMarch 10, 2026Danlin Yang, Neha Kamran, Gururaj Shivange, Kavita Kumari, Prabhu Srinivas Yavvari, Viduth K. Chaugule, Vishal Mahajan, Bridget Larkin, Scott R. Brodeur, Joe Erhardt, et al.using a cocktail containing TNF-α (Miltenyi Biotec, Cat# 130–094-024) and IL-1β (Miltenyi Biotec, Cat# 130–093-898), followed by a 40–42 h incubation. Co-culture with CD4+ T cells: in parallel, CD4+ T cells were isolated

(2) The HIV Protease Inhibitor Ritonavir Reverts the Mesenchymal Phenotype Induced by Inflammatory Cytokines in Normal and Tumor Oral Keratinocytes to an Epithelial One, Increasing the Radiosensitivity of Tumor Oral KeratinocytesCancers (Basel)July 30, 2025Silvia Pomella, Lucrezia D’Archivio, Matteo Cassandri, Francesca Antonella Aiello, Ombretta Melaiu, Francesco Marampon, Rossella Rota, Giovanni Barillaripurchased from Sigma-Aldrich (St Louis, MO, USA) and suspended in DMSO. Human recombinant IL-1 beta (130-093-897), IL-6 (130-093-931), and IL-8 (130-122-359) were obtained from Miltenyi Biotec (Bergisch Gladbach,

(3) Interleukin-6 in synovial fluid drives the conversion of DC2s to DC3s in inflammatory arthritisiScienceJuly 18, 2025Annika H. Decker, Lucas L. van den Hoogen, Tom van Oorschot, Liliana Sanchez-Rocha, Martine A. Boks, Ghaith Bakdash, Ranjeny Thomas, Calin D. Popa, Martijn Verdoes, Rogier M. Thurlings, et al.Ruxolitinib (INCB018424),Selleckchem,Cat#941678-49-5 IL-6,Miltenyi Biotec,Cat#130-093-933 TNFα,Miltenyi Biotec,Cat#130-094-014 IL-1β,Miltenyi Biotec,Cat#130-093-898 ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, LEGENDplex™

(4) Endogenous CD4+ T cells that recognize ALK and the NPM1::ALK fusion protein can be expanded from human peripheral bloodCancer Immunol ResApril 2, 2025Serena Stadler, Rafael B. Blasco, Vijay Kumar Singh, Christine Damm-Welk, Amin Ben-Hamza, Carlotta Welters, Leo Hansmann, Roberto Chiarle, Wilhelm Woessmann(PeproTech, #300–03). After 30h, cells were matured by the addition of 10 ng/ml IL-1β (Miltenyi Biotec, #130-093-898), 200 U/ml TNF-α (PeproTech, #300–01A), 1,000 U/ml IL-6 (Miltenyi Biotec, #130-093-932), and 1 μM prostaglandin

(5) Endogenous CD4+ T Cells That Recognize ALK and the NPM1::ALK Fusion Protein Can Be Expanded from Human Peripheral BloodCancer Immunology ResearchApril 2, 2025Serena Stadler, Rafael B. Blasco, Vijay Kumar Singh, Christine Damm-Welk, Amin Ben-Hamza, Carlotta Welters, Leo Hansmann, Roberto Chiarle, Wilhelm Woessmann(PeproTech, #300–03). After 30h, cells were matured by the addition of 10 ng/ml IL-1β (Miltenyi Biotec, #130-093-898), 200 U/ml TNF-α (PeproTech, #300–01A), 1,000 U/ml IL-6 (Miltenyi Biotec, #130-093-932), and 1 μM prostaglandin

(6) Stromal-like Wilms tumor cells induce human Natural Killer cell degranulation and display immunomodulatory properties towards NK cellsOncoimmunologyMarch 8, 2021Cantoni C, Serra M, Parisi E, Azzarone B, Sementa AR, Nasto LA, Moretta L, Candiano G, Bottino C, Ghiggeri GM, et al.of 20 ng/ml IFN-γ (Miltenyi Biotec, 130–096-482) or for 48 h with 20 ng/ml IL-1β (Miltenyi Biotec, 130–093-897) and 10 ng/ml TNF-α (Miltenyi Biotec, 130–094-014). NK cell isolation and culture NK cells were isolated

(7) Monocytic Cytokines in Autoimmune Polyglandular Syndrome Type 2 Are Modulated by Vitamin D and HLA-DQFrontiers in ImmunologyDecember 7, 2020Anna U. Kraus, Marissa Penna-Martinez, Firouzeh Shoghi, Gesine Meyer, Klaus Badenhoop(SigmaAldrich, Taufkirchen, Germany). Monocytes were treated for 24 h, either with 500 IU/ml human IL1b (Ord. no 130-093-897; Miltenyi Biotech) as an inﬂammatory stimulant, and/or 1 × 10−8 mol/L 1,25(OH)2D3 (Enzo, Lörrach, Germany)

(8) Cerebrospinal fluid dynamics modulation by diet and cytokines in ratsFluids and Barriers of the CNSDecember 1, 2020Zerin Alimajstorovic, Ester Pascual-Baixauli, Cheryl A. Hawkes, Basil Sharrack, A. Jane Loughlin, Ignacio A. Romero, Jane E. PrestonBioscience, Cambridge, UK (00220-0-100)",50,Dhungana et al. [1] IL-1β,"Miltenyi Biotech Ltd, Woking, UK (130-093-897)",0.1,Hayakata et al. [16] Leptin,"Sigma-Aldrich, Dorset, UK (L4146)",100,Dhungana et al. [1] Concentrations

(9) The IL-1 Antagonist Anakinra Attenuates Glioblastoma Aggressiveness by Dampening Tumor-Associated InflammationCancersFebruary 13, 2020Hübner M, Effinger D, Wu T, Strauß G, Pogoda K, Kreth FW, Kreth S.Germany), and passaged no more than 10 times. Interleukin-1β (IL-1β) was purchased from Miltenyi (Cat. No. 130-093-897, Bergisch-Gladbach, Germany) and used at the concentration of 10ng/mL. Anakinra (Kineret®, Swedish Orphan

(10) Targeting IRAK1 in T-Cell acute lymphoblastic leukemiaOncotargetJune 1, 2015Charles Dussiau et al.purchased from Merck Millipore (Ref 407602). Recombinant human IL1-β was purchased from Miltenyi (Ref 130-093-897). Apoptosis, cell cycle analysis and growth curves Apoptosis was assessed by flow cytometry using Annexin

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Human IL-1β, premium grade from Miltenyi Biotec

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Human IL-1β, premium grade from Miltenyi Biotec

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