Human GM-CSF, premium grade 130-093-866 from Miltenyi Biotec

(1) Glycosaminoglycan-functionalized hydrogels for sustained delivery of tissue inhibitor of metalloproteinase-3 mediating matrix metalloprotease inhibition and extracellular matrix stabilizationBioactive MaterialsJuly 1, 2026Fabian Junker, Stefan Rupf, Paula Marie Schindler, Cedric Wilden, Mathias Hohl, Gloria Ruiz-Gómez, M. Teresa Pisabarro, Selina Wrublewsky, Caroline Bickelmann, Charlotte Berhorst, et al.further additives for unpolarized control cells or in presence of human GM-CSF (25 ng/mL, Milteyni # 130-093-865) or M-CSF (50 ng/ml, Miltenyi # 130-096-489) for polarization into M1 or M2 macrophages, respectively.

(2) Activation of L-histidine biosynthesis as a new antibiotic strategy against Mycobacterium tuberculosisNat CommunMarch 21, 2026Debbie M. Hunt, João Pedro Pisco, Angela Rodgers, Cesira de Chiara, Anisha Zaveri, Kamila L. Pacholarz, Dimitrios Evangelopoulos, Acely Garza-Garcia, Sabine Ehrt, Dirk Schnappinger, et al.containing 10 ng/mL of human granulocyte-macrophage colony-stimulating factor (hGM-CSF) (Miltenyi, 130-093-867) in untreated petri dishes at 37 °C in a humidified 5% CO2 incubator for a total of 6 days, with an

(3) Inhibition of MAPK p38α overcomes the cancer immunosurveillance defect caused by FPR1 loss-of-function mutationCell Rep MedMarch 17, 2026Yuhong Pan, Liwei Zhao, Jiani Liu, Misha Mao, Ai-Ling Tian, Julie Le Naour, Sarah Adriana Scuderi, Hui Pan, Flora Doffe, Donal Naylor, et al.DNAse I,Sigma-Aldrich,Cat# DN25 D-Biotin,Sigma-Aldrich,Cat# B4639 Recombinant human GM-CSF,Miltenyi Biotec,Cat# 130-093-867 Recombinant Human IFN-α2,Biolegend,Cat# 592706 "Human Sera Type AB, Heat-inactivated",Institut de

(4) Overcoming claudin family homology: discovery of ARC101, a highly potent CLDN6-specific T-cell engager with a novel CD3 binder for ovarian adenocarcinomaMAbsMarch 10, 2026Danlin Yang, Neha Kamran, Gururaj Shivange, Kavita Kumari, Prabhu Srinivas Yavvari, Viduth K. Chaugule, Vishal Mahajan, Bridget Larkin, Scott R. Brodeur, Joe Erhardt, et al.DC Base Medium, R&D Systems, Cat# CCM003) supplemented with 1000 IU/mL GM-CSF (Miltenyi Biotec, Cat# 130–093-866), 1000 IU/mL IL-4 (Miltenyi Biotec, Cat# 130–093-922), and 50 µg/mL gentamicin (Thermo-Fisher Scientific,

(5) What Is the Role of Giant Endosomal Sorting Complexes Required for Transport (ESCRT) Structures in T Cell Activation?Advanced BiologyJanuary 1, 2026Anthi Psoma, Femmy C. Stempels, Rinse de Boer, Geert van den Bogaartinterleukin‐4 (130‐093‐924; Miltenyi), and 450 U/ml granulocyte macrophage colony‐stimulating factor (130‐093‐867; Miltenyi) for 6 days at 37°C and 5% CO2. 4.2 Mixed Lymphocyte Reaction (MLR) MLRs were performed

(6) Immunopeptidome analysis reveals SERPINB3 as an autoantigen driving eczematized psoriasisSci AdvOctober 24, 2025Manja Jargosch, Jomy Kuruvila, Emanuele Scala, Johannes Grosch, Jessica Eigemann, Sophia Wasserer, Shalva Lekiashvili, Nico Trautwein, Daniel Johannes Kowalewski, Alexander Böhner, et al.days in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF; Miltenyi Biotec, 130-093-864, 150 U/ml) and IL-4 (Miltenyi Biotec, 130-093-920, 150 U/ml) as described previously (71). Before coculture

(7) Immunogenicity of single-chain antibodies: germlining of a VHH lowers T-cell activation from epitopes in FR2 and CDR regionsMAbsOctober 13, 2025Remi Giraudet, Adrien Laroche, Benjamin Chalopin, Steven Dubois, Evelyne Correia, Isabelle Staropoli, Olivier Schwartz, Bernard Maillère, Hervé Nozachsupplemented with 1,000 U/mL recombinant human (rh) IL-4 (130–093-924, Miltenyi Biotec) and rhGM-CSF (130–093-867, Miltenyi Biotec). Immature DCs (20,000 per well) were loaded overnight at 37°C with either 1 or 3 µM

(8) IFNγ-mediated suppression of alternative NF-κB in tumor-resident myeloid cells promotes selective recruitment of cytotoxic but not regulatory T cellsFront ImmunolOctober 9, 2025Adam Brinkman, Ravikumar Muthuswamy, Bowen Dong, Robert P. Edwards, Pawel Kalinski#12440-053) supplemented with 10% FBS (Gibco #10082-147) and 1000 IU/mL recombinant human GM-CSF (Miltenyi #130-093-868) to generate macrophages. On day 3 of cultures, half of the media was replenished with fresh IMDM 10%

(9) Phosphorylation of VAMP3 couples IL-6 exocytosis to dendritic cell activationJournal of Cell ScienceOctober 1, 2025Tongxiang Chen, Anthi Psoma, Shweta Mahajan, Martin ter Beest, Peter Linders, Giulia Franciosa, Frans Bianchi, Geert van den van den Bogaart, Harry WarnerMiltenyi, 130-093-924) and granulocyte-macrophage colony-stimulating factor (GM-CSF; 450 µg/ml; Miltenyi ,130-093-867) for 6 days in RPMI (Thermo Fisher Scientific, 11530586) supplemented with 10% serum (FBS; Thermo Fisher

(10) Anti-SARS-CoV-2 Spike IgA2 Induces Inflammation by Human MacrophagesEuropean journal of immunologyOctober 1, 2025Mes L, Veth J, Van Coillie J, Keijser JBD, Mantel E, van der Mast R, Rispens T, Vidarsson G, van Egmond M, den Dunnen J, et al.Entospletinib (GS‐9973),Selleck Chemicals,Cat. S7523 Etomoxir,Sigma‐Aldrich,236020 Human GM‐CSF,Miltenyi Biotec,Cat. 130‐093‐864 Human M‐CSF,Miltenyi Biotec,Cat. 130‐096‐491 Idelalisib,MedChemExpress,HY‐13026 L‐lactate,Sigma‐Aldrich,L7022‐5G Polyinosinic:polycytidylic

(11) Streptococcus pyogenes EVs induce the alternative inflammasome via caspase-4/-5 in human monocytesEMBO ReportsSeptember 8, 2025Kathrin Krause, Sandra Franch Arroyo, Matteo Ugolini, Tonya Kueck, Timothy J Sullivan, Eric J C Gálvez, Matthias Muenzner, Christian Goosmann, Volker Brinkmann, Christian K Frese, et al.EasySep Human Monocyte Isolation Kit,EasySep Human Monocyte Isolation Kit,Stemcell,19359,19359 Human GM-CSF,Human GM-CSF,Miltenyi Biotec,130-093-866,130-093-866 LDH-Cytotoxicity Assay Kit II,LDH-Cytotoxicity Assay Kit II,Abcam,ab65393,ab65393 P3 Primary

(12) hafoe: an interactive tool for the analysis of chimeric AAV libraries after random mutagenesisGene TherapyJuly 8, 2025Tatevik Jalatyan, Erik Aznauryan, Rokib Hasan, Valeri Vardanyan, Stepan Nersisyan, David B. Thompson, Noah Davidsohn, Sanya Thomas, Simon van Haren, Jenny Tam, et al.(Cellgenix GMBH, 20801-0500) with 250 IU/mL IL-4 ((Miltenyi, 130-093-922), and 800 IU/mL GM-CSF (Miltenyi, 130-093-866), and incubated in a 6-well plate at 37 °C and 5% CO2 for 5 days to generate Mo-DCs. On day 5, 2 × 106

(13) Transplants foster B cell alloimmunity by relaying extracellular vesicles to follicular dendritic cellsCell reportsJune 24, 2025Chen R, Powell JS, Shufesky WJ, Bardhi E, Sullivan MLG, Tkacheva OA, Camirand G, Goncalves Z, Stolz DB, Chalasani G, et al.214-14 Recombinant Mouse TNF-α,Prepotech,Cat # 315-01A Recombinant Human GM-CSF,Miltenyi Biotec,Cat # 130-093-866 Recombinant Human IL-4,Miltenyi Biotec,Cat # 130-093-922 Recombinant Human IFN-γ1b,Miltenyi Biotec,Cat

(14) A crosstalk between adhesion and phagocytosis integrates macrophage functions into their microenvironmentiScienceApril 18, 2025Depierre M, Mularski A, Ruppel A, Le Clainche C, Balland M, Niedergang F.(Gibco, 11140035) and 100 μg/mL penicillin/streptomycin (Gibco, 15140122)) with 4 ng/mL GM-CSF (Miltenyi, 130-093-865) and 0.5 ng/mL M-CSF (Miltenyi, 130-096-491). Medium and cytokines were renewed on day 3 or 4 of differentiation.

(15) Glucose deprivation and identification of TXNIP as an immunometabolic modulator of T cell activation in cancerFront ImmunolApril 7, 2025Agathe Dubuisson, Adèle Mangelinck, Samantha Knockaert, Adrien Zichi, Etienne Becht, Wendy Philippon, Sandra Dromaint-Catesson, Manon Fasquel, Fabien Melchiore, Nicolas Provost, et al.Gibco), 50 ng/mL interleukin-4 (IL-4, cat no. 130-093-922, Miltenyi) and 100 ng/mL GM-CSF (cat no. 130-093-866, Miltenyi) (25). On day 4, 20% of MoDC media was added to the flasks. After 7 days, and prior to co-culture,

(16) Tafazzin regulates neutrophil maturation and inflammatory responseEMBO ReportsFebruary 17, 2025Przemysław Zakrzewski, Christopher M Rice, Kathryn Fleming, Drinalda Cela, Sarah J Groves, Fernando M Ponce-Garcia, Willem Gibbs, Kiran Roberts, Tobias Pike, Douglas Strathdee, et al.SCF,Miltenyi Biotec,130-096-695 Recombinant human IL-3,Bio-Techne,203-IL-010/CF Human GM-SCF,Miltenyi Biotec,130-093-864 Human G-CSF,Miltenyi Biotec,130-096-347 TruCutTM Cas9 Protein v2,Invitrogen,A36400 P3 Primary Cell

(17) CENCAT enables immunometabolic profiling by measuring protein synthesis via bioorthogonal noncanonical amino acid taggingCell Reports MethodsOctober 21, 2024Frank Vrieling, Hendrik J.P. van der Zande, Britta Naus, Lisa Smeehuijzen, Julia I.P. van Heck, Bob J. Ignacio, Kimberly M. Bonger, Jan Van den Bossche, Sander Kersten, Rinke StienstraPlus,Merck,Cat#GE17-1440-03 Recombinant Human M-CSF,Miltenyi Biotec,Cat#130-096-489 Recombinant Human GM-CSF,Miltenyi Biotec,Cat#130-093-864 Collagenase type II from Clostridium histolyticum,Merck,Cat#C6885 Collagenase V,Merck,Cat#C9263 Dispase

(18) Extracellular Silica Nanomatrices Promote In Vitro Maturation of Anti‐tumor Dendritic Cells via Activation of Focal Adhesion KinaseAdvanced MaterialsSeptember 13, 2024Sze Wah Tam, Allen Ka Loon Cheung, Ping Qin, Shiqing Zhang, Zhifeng Huang, Ken Kin Lam YungAIM‐V medium supplemented with 25 ng mL−1 recombinant human GM‐CSF and IL‐4 (Miltenyi Biotec, Cat# 130‐093‐866 and 130‐093‐922). After 24 h of culture, the monocytes were differentiated into the immature hu‐moDCs,

(19) S100A9 and HMGB1 orchestrate MDSC-mediated immunosuppression in melanoma through TLR4 signalingJournal for immunotherapy of cancerSeptember 11, 2024Özbay Kurt FG, Cicortas BA, Balzasch BM, De la Torre C, Ast V, Tavukcuoglu E, Ak C, Wohlfeil SA, Cerwenka A, Utikal J, et al.ab167718, Abcam) or rS100A9 (5 µg/mL; catalog ab287938, Abcam) in the presence of GM-CSF (40 ng/mL; catalog 130-093-865; Miltenyi Biotec) for 72 hours. Monocytes cultured with granulocyte-macrophage colony-stimulating factor

(20) Peculiar transcriptional reprogramming with functional impairment of dendritic cells upon exposure to transformed HTLV-1-infected cellsPLoS pathogensSeptember 1, 2024Carcone A, Mortreux F, Alais S, Mathieu C, Journo C, Dutartre H.31350–010; 0.05 mM). MDDC medium was supplemented with IL-4 and GM-CSF (Miltenyi Biotec, 130-093-922 and 130-093-866; 100 ng/mL each) for differentiation. On day 3, the culture medium was refreshed by discarding half

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2/17/2016Differentiation of M1 Macrophages from Human PBMCs / Monocytes Max HübnerHere, our aim was to to differentiate M1 Macrophages from human PBMCs after extracting monocytes with the Pan Monocyte Isolation Kit II, human using different concentrations of GM-CSF.

Human GM-CSF, premium grade from Miltenyi Biotec

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Miltenyi Biotec

Miltenyi Biotec

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Human GM-CSF, premium grade from Miltenyi Biotec

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