Human FGF-2, premium grade 130-093-564 from Miltenyi Biotec

(1) Distinct 2-phenylimidazo[1,2-a]pyridine derivatives that inhibit breast cancer cell proliferation identified as AHR ligandsiScienceJune 19, 2026Katrin Koellisch, Christine Blattner, Stefano Motta, Janine Wesslowski, Melanie Rothley, Simone Büchel, Savannah Sirounian, Ilenia Segatto, Hanna T. Weber, Julia Müller, et al.factor (EGF),Miltenyi Biotech,Cat#130-093-825 Basic fibroblast growth factor,Miltenyi Biotech,Cat#130-093-840 Hydrocortisone,Sigma Aldrich,Cat# H0135 Choleratoxin,Sigma Aldrich,Cat# C8052 Lipofectamine 3000,Thermo Fisher

(2) Tumor-like proliferation of CCM3 knockout endothelial cells: insights from semaxinib treatment and transcriptome profiling of co-culturesActa Neuropathol CommunApril 20, 2026Valeriia V. Saenko, Janne L. Feldmann, Dariush Skowronek, Debora Singer, Ole J. Schamuhn, Doreen Biedenweg, Sander Bekeschus, Ute Felbor, Matthias Rath, Robin A. PilzMerck Millipore, Darmstadt, Germany) supplemented with 1 ng/ml fibroblast growth factor 2 (FGF-2) (#130-093-564, Miltenyi Biotec, Bergisch Gladbach, Germany). The TargetMol cytokine inhibitor library (#L3600, TargetMol

(3) Cell-type-targeted mitochondrial transplantation rescues cell degenerationNatureApril 15, 2026Temurkhan Ayupov, Verónica Moreno-Juan, Serena Curtoni, Alex Fratzl, Upnishad Sharma, Susana Posada-Céspedes, Ramona Ratiu, Rei Morikawa, Alexandra Graff Meyer, Margherita Pezzoli, et al.containing 15% inactivated FBS (Merck, 21C662-A), 100 ng ml−1 VEGF-A and 100 ng ml−1 FGF-2 (Miltenyi Biotec, 130-093-564). On day 11, individual blood vessel organoids were dissected out of the gel and maintained in 96-well

(4) Establishment of human-relevant in vitro models using animal-free serum replacement and recombinant antibodiesFrontiers in ToxicologyMarch 20, 2026Zahra Miri, Johanna Laakkonen, Emilia Toivonen, Niina Väljä, Susanna Miettinen, Hanna Vuorenpääsupplemented with 5% HS (Serana, Germany), 5 ng/mL basic fibroblast growth factor-2 (bFGF-2, product no.130-093-840, Miltenyi Biotec, United States of America), and 100 U/ml penicillin and 100 μg/mL streptomycin (Pen/Strep,

(5) 5-Azacitidine Partially Resets the Subcellular Localization of YAP in Human Bone Marrow-Derived Mesenchymal Stem CellsCellsMarch 16, 2026Hidehito Takayama, Hisashi Kishi, Gen Kobashifetal bovine serum (FBS, Cytiva, Piscataway, NJ, USA), 1 ng/mL basic fibroblast growth factor (bFGF; 130-093-840, Miltenyi Biotec, Gladbach, Germany), and 100 μg/mL kanamycin (Catalogue number: 15160054, Thermo Fisher Scientific

(6) Coordinated Development of Immune Cell Populations in Vascularized Skin Organoids from Human Induced Pluripotent Stem CellsAdvanced healthcare materialsDecember 1, 2025Mostina M, Sun J, Sim SL, Ahmed IA, Souza-Fonesca-Guimaraes F, Wolvetang EJ, Brown J, Kumari S, Khosrotehrani K, Shafiee A.SB431542 (STEMCELL Technologies, 72234), 10 ng mL−1 fibroblast growth factor 2 (FGF‐2, Miltenyi Biotec, 130‐093‐841), 100 ng mL−1 VEGF, and 1% antibiotic‐antimycotic was added. Day 5 of VO Differentiation: All medium

(7) Mapping cardiac drug transport: In vitro assessment of cardiac P-glycoprotein function with [18F]MC225 by using µ-engineered heart tissuesEuropean Heart Journal OpenNovember 25, 2025Wanling Liu, Maureen Dannenberg, Pascalle Mossel, Zeyu Cai, Inês Farinha Antunes, Jurgen Sijbesma, Chantal Kwizera, Lu Cao, Andries van der Meer, Robert Passier, et al.refreshing the medium to BPEL with TGF-β1 (PeproTech, 100–21) (5 ng/mL) and bFGF-2 (Miltenyi Biotec, 130-093-564) (10 ng/mL) on differentiation day 10 and day 12. Afterwards, cells were maintained in full SmGM-2 medium

(8) PPARγ, a key modulator of metabolic reprogramming, stemness and chemoresistance associated with retrodifferentiation in human hepatocellular carcinomasCell Death & DiseaseNovember 17, 2025Yoann Daniel, Claudine Rauch, Lucille Moutaux, Lise Desquilles, Tifenn Le Charpentier, Karim Fekir, Luis Cano, Daniel Catheline, Servane Pierre, Agnès Burel, et al.0.1 mM β-mercaptoethanol (Gibco, 31350010) and 4 ng/ml fibroblast growth factor 2 (Miltenyi Biotec, 130-093-840). HepaRG-SP was obtained from the progenitor population by sorting cells that are able to efflux Hoescht

(9) Tissue microenvironment dictates the state of human iPSC-derived endothelial cells of distinct developmental origin in 3D cardiac microtissuesiScienceOctober 17, 2025Xu Cao, Maria Mircea, Sara Cascione, Atoosa Amel, Theano Tsikari, Francijna E. van den Hil, Hailiang Mei, Katrin Neumann, Anna Alemany, Konstantinos Anastassiadis, et al.,Sigma Aldrich,Cat#R2625 "TGFb inhibitor, SB431542",Tocris,Cat#1614/10 "Human FGF-2, premium grade",Miltenyi Biotec,Cat#130-093-842 Fibroblast Growth Medium 3,PromoCell,Cat#C-23025 CryoStor CS10 medium,Stem Cell Technologies,Cat#07930 Puromycin dihydrochloride

(10) Modeling common Alzheimer’s disease with high and low polygenic risk in human iPSC: A large-scale research resourceStem Cell ReportsAugust 12, 2025Emily Maguire, Jincy Winston, Sarah H. Ellwood, Rachel O'Donoghue, Bethany Shaw, Atahualpa Castillo Morales, Samuel Keat, Alexandra Evans, Rachel Marshall, Lauren Luckcuck, et al.[Gibco, 15140-122], 55 μM 2-mercaptoethanol [Gibco, 31350-010], and 5 ng/mL bFGF [Miltenyi Biotec, 130-093-842]). Daily 50% medium changes were carried out, and from day 10, MEF-conditioned medium was used. Colonies

(11) Biomarkers for Early Detection and Monitoring of Abnormal Brain Development in Mild Fetal Growth RestrictioniScienceJuly 30, 2025Atsuto Onoda, Yuma Kitase, Jacques-Olivier Coq, Kazuto Ueda, Shinobu Shimizu, Masahiro Tsuji, Masahiro Hayakawa, Yoshiaki SatoLouis, MO) with 10% fetal bovine serum (SH30910.03, Hyclone, Logan, UT), 1 ng/mL human fibroblast GF-2 (130-093-840, Miltenyi Biotec, Bergisch Gladbach, Germany), 2 mM GlutaMAX I (Thermo Fisher Scientific Inc., Waltham,

(12) High-throughput differentiation of human blood vessel organoids reveals overlapping and distinct functions of the cerebral cavernous malformation proteinsAngiogenesisJune 6, 2025Skowronek D, Pilz RA, Saenko VV, Mellinger L, Singer D, Ribback S, Weise A, Claaßen K, Büttner C, Brockmann EM, et al.30 min. 80 µL of StemPro-34 SFM supplemented with 15% Panexin CD, 100 ng/mL FGF-2 (Miltenyi Biotec, #130-093-564), and 100 ng/mL VEGF-A were carefully added to the center of each well. The plate was incubated at 37

(13) Proteomic profiling and functional analysis of extracellular vesicles from metastasis-competent circulating tumor cells in colon cancerJ Exp Clin Cancer ResMarch 22, 2025Luis Enrique Cortés-Hernández, Zahra Eslami-S, Aurore Attina, Silvia Batista, Laure Cayrefourcq, Jérôme Vialeret, Dolores Di Vizio, Christophe Hirtz, Bruno Costa-Silva, Catherine Alix-PanabièresL-glutamine (2 mM, Eurobio™, CSTGLU00_0U), and fibroblast growth factor-2 (10 ng/mL, Milteny Biotec™, 130-093-840, Germany) at 5% CO2 and 37 °C. The CTC-MCC-41 line was transfected with a lentivirus probe expressing

(14) HDAC3 genetic and pharmacologic inhibition radiosensitizes fusion positive rhabdomyosarcoma by promoting DNA double-strand breaksCell death discoveryAugust 6, 2024Cassandri M, Porrazzo A, Pomella S, Noce B, Zwergel C, Aiello FA, Vulcano F, Milazzo L, Camero S, Pajalunga D, et al.(Thermo Fisher Scientific, Lafayette, CO, USA), 1% penicillin-streptomycin, 20 ng/mL EGF (130-097-749) and 20 ng/mL bFGF (130-093-840) both from Miltenyi (Miltenyi Biotec, Bergisch Gladbach, Germany) and plated in a low attachment 6-well

(15) Patient-derived tumoroids and proteomic signatures: tools for early drug discoveryFrontiers in ImmunologyApril 18, 2024Hélène Lê, Jules Deforges, Pasquale Cutolo, Anissa Lamarque, Guoqiang Hua, Véronique Lindner, Shreyansh Jain, Jean-Marc Balloul, Nadia Benkirane-Jessel, Eric Quéméneurthe final volume B27 50X,17504-044,1X VEGF-165,H9166-10µg,50ng/mL HGF,SRP6014-10µg,30ng/mL FGF-2,130-093-839-10µg,20ng/mL EGF,GF316-500µg,100ng/mL IL-2,"130-097-744, Miltenyi Biotech",20UI/mL PDTs and PDHOs

(16) A microfluidic platform integrating functional vascularized organoids-on-chipNature CommunicationsFebruary 16, 2024Clément Quintard, Emily Tubbs, Gustav Jonsson, Jie Jiao, Jun Wang, Nicolas Werschler, Camille Laporte, Amandine Pitaval, Thierno-Sidy Bah, Gideon Pomeranz, et al.F1051), 100 ng/ml VEGF-A (PrepoTech, cat. no. 100-20) and 100 ng/ml FGF-2 (Miltenyi Biotec, cat. no. 130-093-564). Organoids with a diameter of 500-600 µm were selected and added to the microfluidic chip at day 15.

(17) Heart and kidney organoids maintain organ-specific function in a microfluidic systemMaterials Today BioDecember 1, 2023Beatrice Gabbin, Viviana Meraviglia, Maricke L. Angenent, Dorien Ward-van Oostwaard, Wendy Sol, Christine L. Mummery, Ton J. Rabelink, Berend J. van Meer, Cathelijne W. van den Berg, Milena Bellin50 ng/ml VEGF (Miltenyi Biotec, Cat. No: 130-109-386) and 5 ng/ml FGF-2 (Miltenyi Biotec, Cat. No: 130-093-842) using a defined ratio of 70:15:15 (CMs:CFs:ECs) in V-shaped culture microplates (Greiner Bio-one, Cat.

(18) Analysis of autophagy deficiency and cytotoxicity in autophagy-deficient human embryonic stem cell-derived neuronsSTAR protocolsSeptember 15, 2023Korsgen ME, Sun C, Seranova E, Zatyka M, Astuti D, Kataura T, Barrett T, Korolchuk VI, Sarkar S.magnesium),Gibco,Cat# 14190094 Glycine,Sigma-Aldrich,Cat# G8898 "Human FGF-2, premium grade",Miltenyi Biotec,Cat# 130-093-842 KnockOut Serum Replacement,Gibco,Cat# 10828010 Laminin,Sigma-Aldrich,Cat# L2020 L-glutamine (200 mM

(19) Creation of an iPSC-Based Skeletal Muscle Model of McArdle Disease Harbouring the Mutation c.2392T>C (p.Trp798Arg) in the PYGM GeneBiomedicinesAugust 31, 2023Victoria Cerrada, Inés García-Consuegra, Joaquín Arenas, M. Esther GallardoHuman recombinant EGF,20 ng/mL,"Stemcell technologies, Canada, 78006.1" Human bFGF-2,20 ng/mL,"Miltenyi, Germany, 130-093-839" Human recombinant HGF,10 ng/mL,"Stemcell technologies, Canada, 78019.1" Dexamethasone,10 µM,"Sigma Aldrich

(20) In vitro cross-talk between metastasis-competent circulating tumor cells and platelets in colon cancer: a malicious association during the harsh journey in the bloodFront Cell Dev BiolAugust 2, 2023Zahra Eslami-S, Luis Enrique Cortés-Hernández, Ilias Glogovitis, Mafalda Antunes-Ferreira, Silvia D'Ambrosi, Keerthi Kurma, Françoise Garima, Laure Cayrefourcq, Myron G. Best, Danijela Koppers-Lalic, et al.factor (Miltenyi Biotec ref: 130-093-825), and beta fibroblast growth factor (Miltenyi Biotec ref: 130-093-840). They were maintained in a cell culture incubator at 37°C and 5% CO2. To ensure that the CTC lines

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Human FGF-2, premium grade from Miltenyi Biotec

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Human FGF-2, premium grade from Miltenyi Biotec

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