Recombinant Human FGF-8a Protein, CF 4745-F8-050 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemRecombinant Human FGF-8a Protein, CF
CompanyR&D Systems, a Bio-Techne Brand
Price $408.00Supplier Page View Company Product Page
Catalog Number4745-F8-050
Size50 ug
ApplicationsBioactivity
FormatLyophilized from a 0.2 µm filtered solution in MOPS, Na2SO4, and EDTA
Purity>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
Molecular Weight21 kDa
Conjugate/TagUnconjugated
SpeciesHuman
Molecule NameFGF-8
Source/Expression SystemE. coli-derived Recombinant Human FGF-8a Protein, CF
NCBI Full Gene Namefibroblast growth factor 8
NCBI Gene AliasesHBGF-8, AIGF, HH6, KAL6, FGF-8
Storage12 months from date of receipt, <= -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, <= -20 °C under sterile conditions after reconstitution

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Neural regulation of H3K27me3 during the induction of patterning competency in regenerating Axolotl limb cellsCommun BiolApril 24, 2025Michael J. Raymond, Matthew A. Cherubino, Warren A. Vieira, Sheyla Manon, Catherine D. McCuskerng/μL BMP2 + FGF2 + FGF8, or 300 ng/μL BMP2 + FGF8 of each growth factor (BMP2:355-BM-050/CF, FGF8a:4745-F8-050, FGFbasic:233-FB-025/CF, R&D Systems) and implanted into the wound site as previously described20. For

(2) A mitochondrial inside-out iron-calcium signal reveals drug targets for Parkinson's diseaseCell reportsDecember 26, 2023Bharat V, Durairaj AS, Vanhauwaert R, Li L, Muir CM, Chandra S, Kwak CS, Le Guen Y, Nandakishore P, Hsieh CH, et al.(450-02, Peprotech), 200 μM ascorbic acid (A5960, Sigma-Aldrich), 500 nM SAG, and 100 ng mL−1 FGF8a (4745-F8–050, R&D Systems). On day 16–17, neurons were split and transferred onto Matrigel (354277, Corning)-coated

(3) LGALS3 (galectin 3) mediates an unconventional secretion of SNCA/α-synuclein in response to lysosomal membrane damage by the autophagic-lysosomal pathway in human midbrain dopamine neuronsAutophagyMay 1, 2022Burbidge K, Rademacher DJ, Mattick J, Zack S, Grillini A, Bousset L, Kwon O, Kubicki K, Simon A, Melki R, et al.ReproCell, 04–0009) and recombinant human FGF8 (fibroblast growth factor 8; 100 pg/µl; R&D Systems, 4745-F8-050) for 48 h, and KSRM containing LDN, SB, SHH, purmorphamine (Reprocell, 04–0009), FGF8, and CHIR99021

(4) Identification of ASCL1 as a determinant for human iPSC-derived dopaminergic neuronsScientific reportsNovember 15, 2021Earley AM, Burbulla LF, Krainc D, Awatramani R.(R&D Systems 1845-SH-100), 2 μM Purmorphamine (Purm) (Reprocell 04-0009), 100 ng/mL FGF8a (R&D Systems 4745-F8-050). On D3-D4 differentiation medium contained LDN + SB + Shh-C24II + Purm + FGF8a and 3 μM CHIR-99021

(5) A mitochondrial membrane-bridging machinery mediates signal transduction of intramitochondrial oxidationNature metabolismSeptember 1, 2021Li L, Conradson DM, Bharat V, Kim MJ, Hsieh CH, Minhas PS, Papakyrikos AM, Durairaj AS, Ludlam A, Andreasson KI, et al.(450–02, Peprotech), 200 μM Ascorbic Acid (A5960, Sigma-Aldrich), 500 nM SAG, and 100 ng/ml FGF8a (4745-F8–050, R&D systems). On day 17, neurons were transferred onto poly-ornithine and laminin-coated glass coverslips

(6) Protocol for measurement of calcium dysregulation in human induced pluripotent stem cell-derived dopaminergic neuronsSTAR protocolsJune 18, 2021Yin X, Kim JW, Liu S, Dawson TM, Dawson VL.ESC-qualified",Thermo Fisher Scientific,Cat# 10439024 FGF2,PeproTech,Cat# 100-18B FGF8a,R&D Systems,Cat# 4745-F8-050 GDNF,R&D Systems,Cat# 212-GD-010/CF Glutamax,Thermo Fisher Scientific,Cat# 35050061 Knockout serum replacement

(7) A monolayer hiPSC culture system for autophagy/mitophagy studies in human dopaminergic neuronsAutophagyApril 1, 2021Stathakos P, Jiménez-Moreno N, Crompton LA, Nistor PA, Badger JL, Barbuti PA, Kerrigan TL, Randall AD, Caldwell MA, Lane JD.

(8) Defective metabolic programming impairs early neuronal morphogenesis in neural cultures and an organoid model of Leigh syndromeNature communicationsMarch 26, 2021Inak G, Rybak-Wolf A, Lisowski P, Pentimalli TM, Jüttner R, Glažar P, Uppal K, Bottani E, Brunetti D, Secker C, et al.with the addition of ascorbic acid [100 µM], FGF8 [100 ng/ml] (R&D Systems, Minneapolis, MN, USA, #4745-F8-050), PMA [0.5 µM]. After 7 days, we replaced the media condition with: Neurobasal:DMEM/F12 [1:1], N2 [1x],

(9) Single-Cell Transcriptomics of Parkinson's Disease Human In Vitro Models Reveals Dopamine Neuron-Specific Stress ResponsesCell reportsOctober 13, 2020Fernandes HJR, Patikas N, Foskolou S, Field SF, Park JE, Byrne ML, Bassett AR, Metzakopian E.

(10) Defects in mRNA Translation in LRRK2-Mutant hiPSC-Derived Dopaminergic Neurons Lead to Dysregulated Calcium HomeostasisCell stem cellOctober 1, 2020Kim JW, Yin X, Jhaldiyal A, Khan MR, Martin I, Xie Z, Perez-Rosello T, Kumar M, Abalde-Atristain L, Xu J, et al.Universal miRNA Cloning Linker,NEB,Cat#S1315S CircLigase II,Epicentre,Cat#CL9021K Matrigel,Corning,Cat#356231 FGF-8a,R&D Systems,Cat#4745-F8-050 SHH (C25II),R&D Systems,Cat#464-SH-025 LDN193189,Stemgent,Cat#04-0074 SB431542,R&D Systems,Cat#1614/1 CHIR99021,Stemgent,Cat#04-0004 Purmorphamine,Sigma,Cat#SML0868 BDNF,R&D Systems

(11) Fibroblast growth factor 8b induces uncoupling protein 1 expression in epididymal white preadipocytesScientific reportsJune 11, 2019Westphal S, Gantert T, Kless C, Hüttinger K, Klingenspor M, Fromme T.

(12) Felodipine induces autophagy in mouse brains with pharmacokinetics amenable to repurposingNature communicationsApril 18, 2019Siddiqi FH, Menzies FM, Lopez A, Stamatakou E, Karabiyik C, Ureshino R, Ricketts T, Jimenez-Sanchez M, Esteban MA, Lai L, et al.

(13) The signalling receptor MCAM coordinates apical-basal polarity and planar cell polarity during morphogenesisNature communicationsJune 7, 2017Gao Q, Zhang J, Wang X, Liu Y, He R, Liu X, Wang F, Feng J, Yang D, Wang Z, et al.recombinant was from California Bioscience. The recombinant FGF4 (AF-100-31) was from Peprotech, and FGF8a (4745-F8-050) was from R&D Systems. The recombinant human proteins of FGF2 (10014-HNAE), FGFR1/Fc (10616-H03H), FGFR2/Fc

(14) Human iPSC-Derived Neural Progenitors Are an Effective Drug Discovery Model for Neurological mtDNA DisordersCell stem cellMay 4, 2017Lorenz C, Lesimple P, Bukowiecki R, Zink A, Inak G, Mlody B, Singh M, Semtner M, Mah N, Auré K, et al.

(15) Physiological characterisation of human iPS-derived dopaminergic neuronsPloS oneJanuary 1, 2014Hartfield EM, Yamasaki-Mann M, Ribeiro Fernandes HJ, Vowles J, James WS, Cowley SA, Wade-Martins R.

(16) Altered splicing of FGFR1 is associated with high tumor grade and stage and leads to increased sensitivity to FGF1 in bladder cancerThe American journal of pathologyNovember 1, 2010Tomlinson DC, Knowles MA.

(17) Similar expression to FGF (Sef) inhibits fibroblast growth factor-induced tumourigenic behaviour in prostate cancer cells and is downregulated in aggressive clinical diseaseBritish journal of cancerDecember 1, 2009Darby S, Murphy T, Thomas H, Robson CN, Leung HY, Mathers ME, Gnanapragasam VJ.