R-Spondin 1 Protein 3474-RS-050 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemR-Spondin 1 Protein
CompanyR&D Systems, a Bio-Techne Brand
Price $492.00Supplier Page View Company Product Page
Catalog Number3474-RS-050
FormatLyophilized from a 0.2 µm filtered solution in PBS
SpeciesMouse
Size50 ug (also 250 ug, 1 mg)
Molecule NameR-Spondin 1
Purity>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
Molecular Weight21 kDa
ApplicationsBioactivity
Conjugate/TagUnconjugated
Source/Expression SystemE. coli-derived Recombinant Mouse R-Spondin 1 Protein, CF
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Gene AliasesRspondin, R-spondin, RSPO, CRISTIN3
NCBI Full Gene NameR-spondin 1

Specifications/Features

Available conjugates: Sizes Available: 50 ug (also 250 ug, 1 mg)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Obese adipose tissue-derived extracellular vesicles enriched with glycolytic cargo promote colorectal cancer tumorigenesisCancer LettersDecember 28, 2025Parsa S. Haque, Sheré L. Paris, Rhonda F. Souza, Joseph C. Onyiah, Christina Coughlan, David J. Orlicky, Janos Zempleni, Arianne L. TheissHEPES, 1X N2 supplement, 1ng/ul EGF, 2 ng/ul Noggin (250-38, PeproTech), 10 ng/μl murine R-spondin (3474-RS-050, R&D Systems). Tumoroids were treated with veh, N-OB and OB EV at 10^7 EV particles/ml on day 0 and

(2) Generation of Intestinal Epithelial Monolayers From Single-Cell Dissociated OrganoidsBio ProtocOctober 5, 2025Neta Felsenthal, Danijela Matic Vignjevic17844573) 8. Murine Noggin (Peprotech, catalog number: 250-38) 9. R-spondin (R&D Systems, catalog number: 3474-RS-050) 10. Nicotinamide (Sigma-Aldrich, CAS: 98-92-0) 11. Chir-99021 (Euromedex, catalog number: AB-M1692-100MG;

(3) Generation of Intestinal Epithelial Monolayers From Single-Cell Dissociated OrganoidsBIO-PROTOCOLOctober 5, 2025Neta Felsenthal, Danijela Matic Vignjevic17844573) 8. Murine Noggin (Peprotech, catalog number: 250-38) 9. R-spondin (R&D Systems, catalog number: 3474-RS-050) 10. Nicotinamide (Sigma-Aldrich, CAS: 98-92-0) 11. Chir-99021 (Euromedex, catalog number: AB-M1692-100MG;

(4) Epithelium intrinsic zinc sensor controls immune homeostasis with gut microbes via regulation of Tuft cell lineagebioRxiv : the preprint server for biologyMay 12, 2025Yunker R, Han G, Vasquez J, Baldaro K, Zhang L, Lee S, Vaishnava S.

(5) Obese Adipose Tissue Extracellular Vesicles Activate Mitochondrial Fatty Acid β-oxidation to Drive Colonic StemnessCellular and Molecular Gastroenterology and HepatologyMarch 22, 2025Parsa S. Haque, Desiree Goodman, Thor Kuusivuori-Robinson, Christina Coughlan, Yaritza Delgado-Deida, Joseph C. Onyiah, Janos Zempleni, Arianne L. Theiss1 ng/μL EGF (2028-EG, R&D Systems), 2 ng/μL Noggin (250-38, PeproTech), 10 ng/μL murine R-spondin (3474-RS-050, R&D Systems), Y27632 (ALX-270-333-M025, Enzo Life Sciences), and 100 ng/mL (standard concentration)

(6) Collagen signaling and matrix stiffness regulate multipotency in glandular epithelial stem cells in miceNature communicationsDecember 18, 2024Jiang C, Centonze A, Song Y, Chrisnandy A, Tika E, Rezakhani S, Zahedi Z, Bouvencourt G, Dubois C, Van Keymeulen A, et al.

(7) LGR signaling mediates muscle-adipose tissue crosstalk and protects against diet-induced insulin resistanceNature communicationsJuly 20, 2024Kubrak O, Jørgensen AF, Koyama T, Lassen M, Nagy S, Hald J, Mazzoni G, Madsen D, Hansen JB, Larsen MR, et al.

(8) An Adaptable Protocol to Generate a Murine Enteroid-Macrophage Co-Culture SystemInternational journal of molecular sciencesJuly 20, 2024Hentschel V, Govindarajan D, Seufferlein T, Armacki M.315-09, PeproTech (part of Thermo Fisher, Weil am Rhein, Germany) (lot. no. 0519179 L1219).R-Spondin 1, # 3474-RS-050, R&D Systems, Mineapolis, MN, USA (lot. no. OHO5420011).Murine Noggin, # 250-38, PeproTech (part of

(9) Isthmus progenitor cells contribute to homeostatic cellular turnover and support regeneration following intestinal injuryCellJune 6, 2024Ermanno Malagola, Alessandro Vasciaveo, Yosuke Ochiai, Woosook Kim, Biyun Zheng, Luca Zanella, Alexander L.E. Wang, Moritz Middelhoff, Henrik Nienhüser, Lu Deng, et al.(Sigma-Aldrich, A9165), EGF (Thermo Fisher Scientific, PMG8043), Noggin (PeproTech, 250-38), Rspo1 (R&D Systems, 3474-RS-050) supplemented with CHIR (Sigma-Aldrich, SML1046) was changed every two days. Number of organoids was

(10) Noninvasive Stratification of Colon Cancer by Multiplex PET ImagingClinical cancer research : an official journal of the American Association for Cancer ResearchApril 15, 2024Malviya G, Lannagan TRM, Johnson E, Mackintosh A, Bielik R, Peters A, Soloviev D, Brown G, Jackstadt R, Nixon C, et al.

(11) The porcine islet-derived organoid showed the characteristics as pancreatic ductScientific reportsMarch 16, 2024Sakata N, Yoshimatsu G, Kawakami R, Nakano K, Yamada T, Yamamura A, Nagashima H, Kodama S.

(12) Pathway level subtyping identifies a slow-cycling biological phenotype associated with poor clinical outcomes in colorectal cancerNature geneticsMarch 1, 2024Malla SB, Byrne RM, Lafarge MW, Corry SM, Fisher NC, Tsantoulis PK, Mills ML, Ridgway RA, Lannagan TRM, Najumudeen AK, et al.

(13) Microbial-Dependent Recruitment of Immature Myeloid Cells Promotes Intestinal RegenerationCellular and molecular gastroenterology and hepatologyJanuary 1, 2024Jiang Z, Waterbury QT, Malagola E, Fu N, Kim W, Ochiai Y, Wu F, Guha C, Shawber CJ, Yan KS, et al.

(14) Integrative genome-scale analyses reveal post-transcriptional signatures of early human small intestinal development in a directed differentiation organoid modelBMC genomicsOctober 26, 2023Hung YH, Capeling M, Villanueva JW, Kanke M, Shanahan MT, Huang S, Cubitt R, Rinaldi VD, Schimenti JC, Spence JR, et al.Noggin (PeproTech, Rocky Hill, NJ; 250 − 38), 250 ng/µL murine R-spondin (R&D Systems, Minneapolis, MN; 3474-RS-050), and 10 µM Y27632 (Enzo Life Sciences, East Farmingdale, NY; ALX270-333). For Wnt suppression studies,

(15) Studying the Epithelial Effects of Intestinal Inflammation In Vitro on Established Murine ColonoidsJournal of Visualized ExperimentsJune 19, 2023Erin Crawford, Heather L. Mentrup, Elizabeth A. Novak, Kevin P. MollenMicroplates, 24 well tissue culture treated, sterile",Greiner Bio-one,5666–2160, R-Spondin,R&D Systems,3474-RS-050,Stock Concentration (0.25 μg/μL); Final Concentration (500 ng/mL); Solvent (D-PBS + 1% BSA) Tryp LE

(16) Vibrio cholerae biofilms use modular adhesins with glycan-targeting and nonspecific surface binding domains for colonizationNature communicationsApril 13, 2023Huang X, Nero T, Weerasekera R, Matej KH, Hinbest A, Jiang Z, Lee RF, Wu L, Chak C, Nijjer J, et al.ng/mL EGF (Thermo Fisher PMG8041), 100 nM LDN-193189 (Cayman, 11802), 1 µg/mL R-spondin 1 (R&D Systems, 3474-RS-050), 10 µM CHIR99021 (Cayman, 13122), and 10 µM Y27632 (Tocris, 1254). 100 µL of resuspended crypts were

(17) Fibroblast-derived EGF ligand neuregulin 1 induces fetal-like reprogramming of the intestinal epithelium without supporting tumorigenic growthDisease models & mechanismsApril 1, 2023Lemmetyinen TT, Viitala EW, Wartiovaara L, Kaprio T, Hagström J, Haglund C, Katajisto P, Wang TC, Domènech-Moreno E, Ollila S.

(18) Tellu - an object-detector algorithm for automatic classification of intestinal organoidsDisease models & mechanismsMarch 1, 2023Domènech-Moreno E, Brandt A, Lemmetyinen TT, Wartiovaara L, Mäkelä TP, Ollila S.

(19) Intestinal epithelial stem cell transplants as a novel therapy for cerebrovascular strokeBrain, behavior, and immunityJanuary 1, 2023Mani KK, El-Hakim Y, Branyan TE, Samiya N, Pandey S, Grimaldo MT, Habbal A, Wertz A, Sohrabji F.

(20) Sucrose-induced hyperglycemia dysregulates intestinal zinc metabolism and integrity: risk factors for chronic diseasesFrontiers in nutritionJanuary 1, 2023Mitchell SB, Hung YH, Thorn TL, Zou J, Baser F, Gulec S, Cheung C, Aydemir TB.(R&D Systems, 2028-EG), 100 ng/μL Noggin (PeproTech, 250-38), 250 ng/μL murine R-spondin (R&D Systems, 3474-RS-050), and 10 μM Y27632 (Enzo Life Sciences, ALX270-333). Cell culture media was changed every 2 days. To

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Recombinant Mouse R-Spondin 1 Protein, CF

R-Spondin 1 Protein

R-Spondin 1 Protein

R-Spondin 1 Protein

R-Spondin 1 Protein

R-Spondin 1 Protein from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

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R-Spondin 1 Protein from R&D Systems, a Bio-Techne Brand

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