Recombinant Human R-Spondin 2 Protein 3266-RS-025 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemRecombinant Human R-Spondin 2 Protein
CompanyR&D Systems, a Bio-Techne Brand
Price $408.00Supplier Page View Company Product Page
Catalog Number3266-RS-025
SpeciesHuman
Molecule NameR-Spondin 2
Size25 ug
NCBI Full Gene NameR-spondin 2
NCBI Gene AliasesHHRRD, CRISTIN2, TETAMS2
FormatLyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein
Molecular WeightObserved: 25-29 kDa, under reducing conditions.
Theoretical: 22 kDa
ApplicationsBioactivity
Conjugate/TagUnconjugated
Source/Expression SystemNS0-derived Recombinant Human R-Spondin 2 Protein
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution
Purity>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Structural insights into Wnt/β-catenin signaling regulation by LGR4, R-spondin, and ZNRF3Nature communicationsOctober 1, 2025Peng Y, Fujimura A, Asami J, Zhang Z, Shimizu T, Ohto U.

(2) Selective and Potent Peptide Binders of RNF43 for Wnt Signaling InhibitionACS Central ScienceSeptember 24, 2025Sunhee Hwang, Paula Flórez Salcedo, Antonion Korcari, John M. Nicoludis, Estefania Martinez Valdivia, Lingling Peng, Aaron T. Balana, Justin Mak, Christopher M. Crittenden, Amin Famili, et al.day, the cells were treated with 50 ng/mL recombinant Wnt3a (5036-WN-010), 25 ng/mL recombinant RSPO2 (3266-RS-025, R&D systems), and monomeric or hexameric DCPs for 6 h. Cells were gently washed with PBS, and RNAs

(3) Rspo2 exacerbates rheumatoid arthritis by targeting aggressive phenotype of fibroblast-like synoviocytes and disrupting chondrocyte homeostasis via Wnt/β-catenin pathwayArthritis research & therapyNovember 9, 2023Guo D, Pan H, Lu X, Chen Z, Zhou L, Chen S, Huang J, Liang X, Xiao Z, Zeng H, et al.

(4) CD276 expression enables squamous cell carcinoma stem cells to evade immune surveillanceCell stem cellSeptember 2, 2021Wang C, Li Y, Jia L, Kim JK, Li J, Deng P, Zhang W, Krebsbach PH, Wang CY.

(5) A Wnt-Independent LGR4-EGFR Signaling Axis in Cancer MetastasisCancer researchSeptember 1, 2021Yue F, Jiang W, Ku AT, Young AIJ, Zhang W, Souto EP, Gao Y, Yu Z, Wang Y, Creighton CJ, et al.human recombinant Wnt3a (R&D Systems 5036-WN-010) or 200 ng/ml human recombinant RSPO2 (R&D Systems 3266-RS-025) or a combination of Wnt3a and RSPO2 for 24 hr before sample collection. For EGF stimulation, cells

(6) RSPO2 inhibits BMP signaling to promote self-renewal in acute myeloid leukemiaCell reportsAugust 17, 2021Sun R, He L, Lee H, Glinka A, Andresen C, Hübschmann D, Jeremias I, Müller-Decker K, Pabst C, Niehrs C.

(7) R-Spondin 2 and WNT/CTNNB1 Signaling Pathways Are Required for Porcine Follicle Development and In Vitro MaturationAnimals : an open access journal from MDPIMarch 5, 2021Hwang SU, Yoon JD, Kim M, Cai L, Choi H, Oh D, Kim E, Hyun SH.signaling on embryo development. 2. Materials and Methods 2.1. Chemicals Recombinant Human R-Spondin 2 (3266-RS-025; R&D System, Minneapolis, MN, USA) was dissolved in 0.1% (wt/vol) Dulbecco’s phosphate-buffered saline

(8) Establishment of 3D Neuro-Organoids Derived from Pig Embryonic Stem-Like CellsInternational Journal of Molecular SciencesMarch 5, 2021Seon-Ung Hwang et al.500 ng/mL sonic hedgehog (SHH; PeproTech, Rocky Hill, NJ, USA), 50 ng/mL FGF8, and 100 ng/mL RSPO2 (3266-RS-025; R&D System, Minneapolis, MN, USA) were added to the DN medium and the neuro-organoids were cultured

(9) Fallopian Tube Basal Stem Cells Reproducing the Epithelial Sheets In Vitro-Stem Cell of Fallopian EpitheliumBiomoleculesSeptember 3, 2020Zhu M, Iwano T, Takeda S.Peprotech), 50 ng/mL Wnt-3a (5036-WN-010, R&D systems, Minneapolis, MN, USA), and 125 ng/mL R-Spondin 2 (3266-RS-025, R&D systems). Finally, the basal medium with 2 ng/mL β-estradiol (E4389, Sigma-Aldrich) was used as

(10) Targeting the Wnt signaling pathway through R-spondin 3 identifies an anti-fibrosis treatment strategy for multiple organsPloS oneJanuary 1, 2020Zhang M, Haughey M, Wang NY, Blease K, Kapoun AM, Couto S, Belka I, Hoey T, Groza M, Hartke J, et al.

(11) ΔN-Tp63 Mediates Wnt/β-Catenin-Induced Inhibition of Differentiation in Basal Stem Cells of Mucociliary EpitheliaCell reportsSeptember 24, 2019Haas M, Gómez Vázquez JL, Sun DI, Tran HT, Brislinger M, Tasca A, Shomroni O, Vleminckx K, Walentek P.

(12) Estrogen and EGFR Pathways Regulate Notch Signaling in Opposing Directions for Multi-Ciliogenesis in the Fallopian TubeCellsAugust 19, 2019Zhu M, Iwano T, Takeda S.Peprotech), 50 ng/mL Wnt-3a (#5036-WN-010, R&D systems, Minneapolis, MN, USA), and 125 ng/mL R-Spondin 2 (#3266-RS-025, R&D systems) into the basal medium. To examine the effect on differentiation, cells were treated with

(13) Differential activities and mechanisms of the four R-spondins in potentiating Wnt/β-catenin signalingThe Journal of biological chemistryJune 22, 2018Park S, Cui J, Yu W, Wu L, Carmon KS, Liu QJ.

(14) Wnt Signalling Drives Context-Dependent Differentiation or Proliferation in NeuroblastomaNeoplasia (New York, N.Y.)April 1, 2018Szemes M, Greenhough A, Melegh Z, Malik S, Yuksel A, Catchpoole D, Gallacher K, Kollareddy M, Park JH, Malik K.

(15) R-spondins can potentiate WNT signaling without LGRseLifeFebruary 6, 2018Lebensohn AM, Rohatgi R.

(16) Upregulation of RSPO2-GPR48/LGR4 signaling in papillary thyroid carcinoma contributes to tumor progressionOncotargetDecember 29, 2017Kang YE, Kim JM, Kim KS, Chang JY, Jung M, Lee J, Yi S, Kim HW, Kim JT, Lee K, et al.thyroid cancer cell lines BCPAP and TPC-1 were treated with recombinant human RSPO2 (RSPO2, R&D Systems, 3266-RS-025). Recombinant RSPO2 was diluted to 100 μg/mL in PBS containing 0.1% bovine serum albumin. Human cell

(17) Aberrantly expressed LGR4 empowers Wnt signaling in multiple myeloma by hijacking osteoblast-derived R-spondinsProceedings of the National Academy of Sciences of the United States of AmericaJanuary 10, 2017van Andel H, Ren Z, Koopmans I, Joosten SP, Kocemba KA, de Lau W, Kersten MJ, de Bruin AM, Guikema JE, Clevers H, et al.

(18) R-spondin 2 promotes acetylcholine receptor clustering at the neuromuscular junction via Lgr5Scientific reportsJune 22, 2016Nakashima H, Ohkawara B, Ishigaki S, Fukudome T, Ito K, Tsushima M, Konishi H, Okuno T, Yoshimura T, Ito M, et al.

(19) Therapeutic Targeting of Tumor-Derived R-Spondin Attenuates β-Catenin Signaling and Tumorigenesis in Multiple Cancer TypesCancer researchFebruary 1, 2016Chartier C, Raval J, Axelrod F, Bond C, Cain J, Dee-Hoskins C, Ma S, Fischer MM, Shah J, Wei J, et al.

(20) RSPO-LGR4 functions via IQGAP1 to potentiate Wnt signalingProceedings of the National Academy of Sciences of the United States of AmericaApril 1, 2014Carmon KS, Gong X, Yi J, Thomas A, Liu Q.

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Recombinant Human R-Spondin 2 Protein

R-Spondin 2 Protein

R-Spondin 2 Protein

R-Spondin 2 Protein

R-Spondin 2 Protein

Recombinant Human R-Spondin 2 Protein from R&D Systems, a Bio-Techne Brand

R&D Systems, a Bio-Techne Brand

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Recombinant Human R-Spondin 2 Protein from R&D Systems, a Bio-Techne Brand

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