Recombinant Human Fc gamma RIIIA/CD16a Protein, CF 4325-FC-050 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemRecombinant Human Fc gamma RIIIA/CD16a Protein, CF
CompanyR&D Systems, a Bio-Techne Brand
Price $465.00Supplier Page View Company Product Page
Catalog Number4325-FC-050
FormatLyophilized from a 0.2 µm filtered solution in PBS
SpeciesHuman
Molecule NameFc gamma RIIIA/CD16a
Size50 ug
Purity>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Molecular WeightObserved: 40-50 kDa, reducing conditions
Theoretical: 22.6 kDa
ApplicationsBinding Activity
Conjugate/TagUnconjugated
Source/Expression SystemNS0-derived Recombinant Human Fc gamma RIIIA/CD16a Protein, CF
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Full Gene NameFc gamma receptor IIIa
NCBI Gene AliasesCD16, CD16-II, CD16A, FCG3, FCGR3, FCGRIII, FCR-10, FCRIII, FCRIIIA, FcGRIIIA, IGFR3, IMD20

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Reduced Fc-mediated antibody responses after COVID-19 mRNA vaccination in a cohort of people living with HIV-1Scientific ReportsNovember 25, 2025Jéromine Klingler, Priyanka Gadam Rao, Juan C. Bandres, Ismael Pena, Katherine Bolanos Roldan, Gagandeep Singh, Brian Monahan, Charles Gleason, Yuexing Chen, Stefan Slamanig, et al.serially diluted serum were incubated with His-tagged recombinant FcγRIIIa/CD16a protein (R&D Systems™, #4325FC050) for one hour at room temperature, followed by an anti-His-PE antibody (R&D Systems™, #IC050P) for 30

(2) Antigenic peptide delivery to antigen-presenting cells using a CD40-coiled coil affinity-based platformDrug DelivMay 26, 2025Barnabas Nyesiga, Karin Hägerbrand, Laura Varas, Anette Gjörloff Wingren, Mats Ohlin, Peter Ellmark, Laura von SchantzR&D Systems), FcγRIIa-R167 (1330-CD, R&D Systems), FcγRIIb (1875-CD-050, R&D Systems), FcγRIIIa-V176 (4325-FC-050, R&D Systems), FcγRIIIa F176 (8894-FC-050, R&D Systems), or mouse FcγRI (2074-FC, R&D Systems), FcγRIIb

(3) CAR T-cell-mediated delivery of bispecific innate immune cell engagers for neuroblastomaNature communicationsAugust 21, 2024Pascual-Pasto G, McIntyre B, Hines MG, Giudice AM, Garcia-Gerique L, Hoffmann J, Mishra P, Matlaga S, Lombardi S, Shraim R, et al.

(4) Heterologous Ad26/Ad5 adenovirus-vectored vaccines elicited SARS-CoV-2-specific antibody responses with potent Fc activitiesFrontiers in ImmunologyMay 8, 2024Jéromine Klingler, Shreyas Kowdle, Juan C. Bandres, Rozita Emami-Gorizi, Raymond A. Alvarez, Priyanka G. Rao, Fatima Amanat, Charles Gleason, Giulio Kleiner, Viviana Simon, et al.(R&D Systems™, #1257FC050), FcγRIIa/CD32a (R&D Systems™, #1330CD050/CF) or FcγRIIIa/CD16a (R&D Systems™, #4325FC050) proteins for 1 hour at room temperature, followed by an anti-His-PE antibody (R&D Systems™, #IC050P)

(5) Bivalent non-human gal-α1-3-gal glycan epitopes in the Fc region of a monoclonal antibody model can be recognized by anti-Gal-α1-3-Gal IgE antibodiesmAbsDecember 31, 2023Grayson Hatfield, Lioudmila Tepliakova, Jessica Tran, Huixin Lu, Michel Gilbert, Roger Y. Tam(Figure S3). FcγRIIIA binding assay by ELISA Recombinant human FcγRIIIA V176 protein (R&D Systems, Cat# 4325-FC-050) was immobilized onto MaxiSorp plates overnight at 4°C, at 50 µL of 2.0 µg/mL. Following washing with

(6) A Dual-Approach Strategy to Optimize the Safety and Efficacy of Anti-Zika Virus Monoclonal Antibody TherapeuticsVirusesMay 11, 2023Sun H, Yang M, Lai H, Neupane B, Teh AY, Jugler C, Ma JK, Steinkellner H, Bai F, Chen Q.

(7) Optimized ACE2 decoys neutralize antibody-resistant SARS-CoV-2 variants through functional receptor mimicry and treat infection in vivoScience advancesDecember 9, 2022Torchia JA, Tavares AH, Carstensen LS, Chen DY, Huang J, Xiao T, Mukherjee S, Reeves PM, Tu H, Sluder AE, et al.

(8) BNT162b2-induced neutralizing and non-neutralizing antibody functions against SARS-CoV-2 diminish with ageCell reportsOctober 25, 2022Bates TA, Lu P, Kang YJ, Schoen D, Thornton M, McBride SK, Park C, Kim D, Messer WB, Curlin ME, et al.

(9) Specific location of galactosylation in an afucosylated antiviral monoclonal antibody affects its FcÎ³RIIIA binding affinityFrontiers in ImmunologyOctober 12, 2022Grayson Hatfield, Lioudmila Tepliakova, Genevieve Gingras, Andrew Stalker, Xuguang Li, Yves Aubin, Roger Y. Tam349454) overnight at 4°C, at 100 µL of 0.33 µg/mL for FcγRIIIA V176 (high affinity, R&D Systems, Cat# 4325-FC-050), or at 0.67 µg/mL for FcγRIIIA V176F (low affinity, R&D, Cat# 8894-FC-050). Following washing with

(10) Discovery of a novel Igβ and FcγRIIB cross-linking antibody, ASP2713, and its potential application in the treatment of systemic lupus erythematosusInternational immunopharmacologyDecember 1, 2021Kitanaga Y, Yamajuku D, Kubo S, Nakamura K, Maeda M, Seki M, Kaneko Y, Kinugasa F, Morokata T, Kondo Y, et al.

(11) Fc-Independent Protection from SARS-CoV-2 Infection by Recombinant Human Monoclonal AntibodiesAntibodies (Basel, Switzerland)November 8, 2021Noy-Porat T, Edri A, Alcalay R, Makdasi E, Gur D, Aftalion M, Evgy Y, Beth-Din A, Levy Y, Epstein E, et al.Israel). 2.3. Binding to Recombinant FcγRs Binding to FcγRs was tested using ELISA. CD16 (CatNo. 4325-FC-050, R&D Systems, Minneapolis, MN, USA) and CD64 (Cat No. 10256-H08S, Sino Biological, Beijing, China) were

(12) High neutralizing potency of swine glyco-humanized polyclonal antibodies against SARS-CoV-2European journal of immunologyJune 1, 2021Vanhove B, Duvaux O, Rousse J, Royer PJ, Evanno G, Ciron C, Lheriteau E, Vacher L, Gervois N, Oger R, et al.

(13) Non-neutralizing Antibodies May Contribute to Suppression of SIVmac239 Viremia in Indian Rhesus MacaquesFrontiers in ImmunologyMarch 16, 2021Nuria Pedreño-Lopez et al.isotype, or PE-conjugated FcγRIIa, FcγRIIb, and FcγRIIIa (R&D Systems, #1330-CD-050, #1875-CD-050, #4325-FC-050, respectively). The plates were incubated for 1 h at room temperature on a shaker. Next, we added goat anti-mouse IgG Fc PE

(14) Preclinical characterization of dostarlimab, a therapeutic anti-PD-1 antibody with potent activity to enhance immune function in in vitro cellular assays and in vivo animal modelsmAbsJanuary 1, 2021Kumar S, Ghosh S, Sharma G, Wang Z, Kehry MR, Marino MH, Neben TY, Lu S, Luo S, Roberts S, et al.dostarlimab to FcγRI (Cat#1257-FC-050), FcγRIIa (Cat#9595-CD-050), FcγRIIb (Cat#1875-CD-050), FcγRIIIa (Cat#4325-FC-050), and FcγRIIIb (Cat#1597-FC-050; all from R&D Systems) was assessed by SPR using Biacore T200 and Biacore

(15) Monoclonal antibody formulation manufactured by high-speed electrospinningInternational journal of pharmaceuticsDecember 15, 2020Domján J, Vass P, Hirsch E, Szabó E, Pantea E, Andersen SK, Vigh T, Verreck G, Marosi G, Nagy ZK.

(16) High Potency of a Bivalent Human V(H) Domain in SARS-CoV-2 Animal ModelsCellOctober 15, 2020Li W, Schäfer A, Kulkarni SS, Liu X, Martinez DR, Chen C, Sun Z, Leist SR, Drelich A, Zhang L, et al.1257-FC-050 Recombinant FcγRIIA,R&D systems,Cat# 1330-CD-050 Recombinant FcγRIIIA,R&D systems,Cat# 4325-FC-050 ,, Critical Commercial Reagents,Critical Commercial Reagents,Critical Commercial Reagents ,, Blitz

(17) Potent Cytolytic Activity and Specific IL15 Delivery in a Second-Generation Trispecific Killer EngagerCancer Immunology ResearchSeptember 1, 2020Martin Felices et al.ligands CD16a and CD16b. The ligands, rhCD16A or rhCD16B with a c-terminal 6x-HIS tag (R&D Systems cat#: 4325-FC-050 and 1597-FC-050/CF, respectively) were captured on biosensor tips using His tag-specific dip and read

(18) Pharmacologic Characterization of ALD403, a Potent Neutralizing Humanized Monoclonal Antibody Against the Calcitonin Gene-Related PeptideJournal of Pharmacology and Experimental TherapeuticsJuly 1, 2020Leon F. Garcia-Martinez et al.FcgRIIA/CD32a, cat. no. 1330CD-050; FcgRIIB/C (CD32b/c), cat. no. 1875-CD-050; FcgRIIIA/ CD16a, cat. no. 4325-FC-050; FcgRIIIB/CD16b, cat. no. 1597-fc-050/ CF; R&D Systems, Minneapolis, MN] were diluted to 7 mg/ml in 20

(19) Antigen-Induced Allosteric Changes in a Human IgG1 Fc Increase Low-Affinity Fcγ Receptor BindingStructureMay 1, 2020Chiara Orlandi, Daniel Deredge, Krishanu Ray, Neelakshi Gohain, William Tolbert, Anthony L. DeVico, Patrick Wintrode, Marzena Pazgier, George K. LewisIIB/CD32b,R&D systems,Cat# 1875-CD-050 Human Recombinant FcγR receptor IIIA/CD16a,R&D systems,Cat# 4325-FC-050 Horseradish peroxidase conjugated Fab’2 goat anti-human IgG (H+L),KPL,Cat# 214-1006 "Chemicals, Peptides,

(20) Insertion of N-Terminal Hinge Glycosylation Enhances Interactions of the Fc Region of Human IgG1 Monomers with Glycan-Dependent Receptors and Blocks Hemagglutination by the Influenza VirusJournal of immunology (Baltimore, Md. : 1950)March 1, 2019Blundell PA, Lu D, Wilkinson M, Dell A, Haslam S, Pleass RJ.

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Recombinant Human Fc gamma RIIIA/CD16a Protein, CF

Fc gamma RIIIA/CD16a Protein

Fc gamma RIIIA/CD16a Protein

Fc gamma RIIIA/CD16a Protein

Fc gamma RIIIA/CD16a Protein

Recombinant Human Fc gamma RIIIA/CD16a Protein, CF from R&D Systems, a Bio-Techne Brand

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R&D Systems, a Bio-Techne Brand

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Recombinant Human Fc gamma RIIIA/CD16a Protein, CF from R&D Systems, a Bio-Techne Brand

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