Annexin V-FITC 130-093-060 from Miltenyi Biotec

(1) Monocyte derived large extracellular vesicles in polytraumaJournal of Extracellular BiologySeptember 2, 2024Aliona Wöhler, Sabine K. Gries, Rebekka J. S. Salzmann, Christina Krötz, Bingduo Wang, Paula Müller, Angelina Klein, Ingo G. H. Schmidt‐Wolf, Sebastian Schaaf, Robert Schwab, et al.us can be found elsewhere (Kornek et al., 2011). Large EVs were stained with Annexin V (AnnV)‐FITC (130‐093‐060, Miltenyi Biotec) and subsequently stained with anti‐CD14‐VioBlue (130‐094‐364, Miltenyi Biotec) (Table

(2) Pharmacological degradation of ATR induces antiproliferative DNA replication stress in leukemic cellsMolecular oncologyAugust 1, 2024Kansy AG, Ashry R, Mustafa AM, Alfayomy AM, Radsak MP, Zeyn Y, Bros M, Sippl W, Krämer OH.Flow cytometry was performed as recently noted by us [9, 10, 11]. Annexin‐V (indicator of early apoptosis; #130‐093‐060) was from Miltenyi Biotec, Bergisch Gladbach, Germany, and propidium iodide (PI; late apoptosis indicator)

(3) Dynamic encounters with red blood cells trigger splenic marginal zone B cell retention and functionNature ImmunologyJanuary 1, 2024Dan Liu, Benjamin Y. Winer, Marissa Y. Chou, Hanson Tam, Ying Xu, Jinping An, James M. Gardner, Jason G. Cyster(REA300, 130-104-023; 1:100), APC-conjugated anti-CD97 (REA678, 130-110-229; 1:100), Annexin V-FITC (130-093-060; 1:200), APC-conjugated anti-CD97 (REA1242, 130-124-980; 1:100) and APC-conjugated anti-CD55 (REA1231,

(4) Histone deacetylase inhibitors modulate hormesis in leukemic cells with mutant FMS-like tyrosine kinase-3LeukemiaNovember 1, 2023Zeyn Y, Hausmann K, Halilovic M, Beyer M, Ibrahim HS, Brenner W, Mahboobi S, Bros M, Sippl W, Krämer OH.for 24-48 h and subjected to flow cytometry analyses for annexin-V (indicator of early apoptosis; #130-093-060 from Miltenyi Biotec) and propidium iodide (PI; from Sigma-Aldrich; late apoptosis indicator). A MV4-11

(5) Human cellular model systems of β-thalassemia enable in-depth analysis of disease phenotypeNature communicationsOctober 6, 2023Daniels DE, Ferrer-Vicens I, Hawksworth J, Andrienko TN, Finnie EM, Bretherton NS, Ferguson DCJ, Oliveira ASF, Szeto JA, Wilson MC, et al.Ltd) and 2 mg.ml−1 glucose (PBS-AG), followed by incubation with Annexin V-FITC conjugated antibody (130-093-060; Miltenyi; dilution 1:11) in Annexin V binding buffer (10 mM Hepes, pH7.4; 140 mM NaCl; 2.5 mM CaCl2).

(6) COPB2 promotes metastasis and inhibits apoptosis of lung adenocarcinoma cells through functioning as a target of miR-216a-3pTranslational Cancer ResearchApril 1, 2020Mingxue Wang, Renjie Yu, Xuefeng Ling, Wa Cao, Yunyun Liu, Lei Fang, Jianlin Tongthen washed in phosphate buffered saline (PBS), centrifuged at 1,000 r/min for 5 min. Five μL Annexin V-FITC (130093-060, Miltenyi, Germany) was added to the cells, gently mixed and incubated for 15 min, the supernatant was discarded,

(7) New Drug Repositioning Candidates for T-ALL Identified Via Human/Murine Gene Signature ComparisonFrontiers in oncologyJanuary 1, 2020Bonnet R, Nebout M, Brousse C, Reinier F, Imbert V, Rohrlich PS, Peyron JF.was performed by measuring phosphatidyl-serine membrane redistribution via annexin-V-FITC staining (130-093-060, Miltenyi Biotec). After incubation with effectors for indicated times, cells were re-suspended in staining

(8) Intrinsic antiproliferative activity of the innate sensor STING in T lymphocytesThe Journal of experimental medicineJune 5, 2017Cerboni S, Jeremiah N, Gentili M, Gehrmann U, Conrad C, Stolzenberg MC, Picard C, Neven B, Fischer A, Amigorena S, et al.resuspended with 50 µl of 1× annexin V binding buffer (no. 51-66121E; BD) and 5 µl annexin V FITC (no. 130-093-060; Miltenyi Biotec). Cells were incubated at room temperature for 15 min. 150 µl of 1× binding buffer

(9) Melanoma Affects the Composition of Blood Cell-Derived Extracellular VesiclesFrontiers in immunologyJanuary 1, 2016Koliha N, Heider U, Ozimkowski T, Wiemann M, Bosio A, Wild S.avoid any contamination with bovine EVs. The percentage of apoptotic cells (AnnexinV+, Miltenyi Biotec, 130-093-060) was below 5%. The donors of whole blood, buffy coats, and leukapheresis that were used for the following

(10) Annexin V binds to positively selected B cellsJournal of immunology (Baltimore, Md. : 1950)January 1, 2001Dillon SR, Constantinescu A, Schlissel MS.

(11) A receptor for phosphatidylserine-specific clearance of apoptotic cellsNatureMay 4, 2000Fadok VA, Bratton DL, Rose DM, Pearson A, Ezekewitz RA, Henson PM.

(12) Quantitative measurement of mast cell degranulation using a novel flow cytometric annexin-V binding assayCytometryAugust 1, 1999Demo SD, Masuda E, Rossi AB, Throndset BT, Gerard AL, Chan EH, Armstrong RJ, Fox BP, Lorens JB, Payan DG, et al.

(13) Detection of altered membrane phospholipid asymmetry in subpopulations of human red blood cells using fluorescently labeled annexin VBloodFebruary 1, 1996Kuypers FA, Lewis RA, Hua M, Schott MA, Discher D, Ernst JD, Lubin BH.

(14) Early redistribution of plasma membrane phosphatidylserine is a general feature of apoptosis regardless of the initiating stimulus: inhibition by overexpression of Bcl-2 and AblThe Journal of experimental medicineNovember 1, 1995Martin SJ, Reutelingsperger CP, McGahon AJ, Rader JA, van Schie RC, LaFace DM, Green DR.

(15) Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosisBloodSeptember 1, 1994Koopman G, Reutelingsperger CP, Kuijten GA, Keehnen RM, Pals ST, van Oers MH.

(16) Exposure of phosphatidylserine on the surface of apoptotic lymphocytes triggers specific recognition and removal by macrophagesJournal of immunology (Baltimore, Md. : 1950)April 1, 1992Fadok VA, Voelker DR, Campbell PA, Cohen JJ, Bratton DL, Henson PM.

(17) Transbilayer movement of phospholipids in red cell and platelet membranesBiochimica et biophysica actaNovember 13, 1991Schroit AJ, Zwaal RF.

(18) Binding of annexin V/placental anticoagulant protein I to platelets. Evidence for phosphatidylserine exposure in the procoagulant response of activated plateletsThe Journal of biological chemistryOctober 15, 1990Thiagarajan P, Tait JF.

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Annexin V-FITC from Miltenyi Biotec

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Annexin V-FITC from Miltenyi Biotec

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