Fig 1: During MCMV Infection, a Subset of IFNARI-Dependent Transcriptional Changes in NK Cells Are STAT1 Independent(A) Schematic of experimental design. Briefly, mBMC mice harboring both WT and Ifnarl or WT and Statl NK cells were infected with MCMV. Splenic Ly49H+ NK cells of each genotype were sorted for RNA-seq at 0 and 1.5 days PI (n = 1–3 per genotype pertime point).(B) Volcano plot of RNA-seq data comparing Ifnar1−/− and WTNK cells at day 1.5PI. IFNa-induciblegenes (i.e., differentially expressed genes in Figure 1) that are also differentially expressed in Ifnar1−/− versus WT NK cells are shown in blue, with select genes highlighted. Number of blue genes of total differentially expressed between Ifnar1−/− and WT NK cells at day 1.5 PI genes indicated at upper right.(C) As in (B) but showing Stat1−/− versus WT.(D) Normalized counts of Stat1, Stat2,and Irf9 in WT and Stat1−/− NK cells from RNA-seq. Symbols represent biological replicates, and error bars show SEM (*p<0.05).(E) Overlap of differentially expressed genes between WT and Ifnar1−/− versus WT and Stat1−/− NK cells at day 1.5 PI.