Fig 1: Western blot demonstrating the generation of HA-HC complexes and their effect on stimulating CSF-2 BMDMs and BMDCs.(a) Representative anti-IαI (HC) Western blot showing the HC of IαI in serum and when bound to HA by TSG-6 and when released by Strp HA’se. (b) Summary graphs of the percent of cells expressing TNF-α and IL-12 p40 production after stimulation with in vitro generated HA-HC complexes or with control samples containing just serum and TSG-6. Graphs show an average of six mice pooled from two experiments ± SD. Significance compared to the unstimulated control indicated as *p < 0.05, **p < 0.01, ***p < 0.001, unpaired student’s t-test.