a2-3,6,8 Neuraminidase P0720S from New England Biolabs

Product Specs
Itema2-3,6,8 Neuraminidase
CompanyNew England Biolabs
Price $77.00 Inquire
Catalog NumberP0720S
ApplicationsProteomics & Glycomics
Molecule NameNeuraminidase
Size2000 units
Concentration50,000 units/ml
Temperature-20 C
TypeGlycosidases

Specifications/Features

Storage:
-20°C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Cancer cell-derived sialylated IgG interacting with Siglec-7/9/10 is a potential immunotherapeutic target in pancreatic cancerCell Rep MedMarch 17, 2026Shenghua Zhang, Ming Cui, Xinmei Huang, Xiaoyao Feng, Ruiling Xiao, Qijia Liu, Jialu Bai, Xianlin Han, Xiaoding Liu, Weiyan Xu, et al.IV,Solarbio,Cat# C8160 DNase I,Solarbio,Cat# D8070 PNGase F,NEB,Cat# P0704 "α 2-3,6,8 Neuraminidase",NEB,Cat# P0720 α 2-3 Neuraminidase,NEB,Cat# P0743 "Human Siglec-9, His Tag",ACRO Biosystems,Cat# SI9-H52H4 CFSE,Thermo Fisher Scientific

(2) Globo H ceramide confers chemoresistance and poor prognosis to advanced gallbladder cancer via A2AR/cAMP/PKA pathwayTheranosticsFebruary 26, 2026Tsai-Hsien Hung, Jung-Tung Hung, Wen-Kuan Huang, Yenlin Huang, Chiao-En Wu, Jing-Yan Cheng, Chien-Wei Lee, John Yu, Yi-Chia Wu, Chun-Nan Yeh, et al.treated with 800 units of β1,4-galactosidase (NEB, P0745L) and 1250 units of α2-3,6,8 neuraminidase (NEB, P0720L) in 1X GlycoBuffer at 37 °C for 24 h. An additional 200 units of β1,4-galactosidase was subsequently

(3) Specific Detection of Sialyltransferase ST3GAL3 Towards Lipid Acceptors by Liquid Chromatography Coupled with Tandem Mass Spectrometry Indicates Total Loss of Enzyme Activity in ST3GAL3 Pathogenic VariantsBiomedicinesFebruary 12, 2026Sara Penati, Michele Dei Cas, Linda Montavoci, Anna Caretti, Marco TrincheraAvanti Polar, Alabaster, AL, USA). nLc4 was obtained by neuraminidase treatment (α2-3,6,8 neuraminidase, P0720S, New England Biolabs, Ipswich, MA, USA) of sialyl neolactotetraosylceramide (snLc4, Neu5Acα(2-3)Galβ(1-4)GlcNAcβ(1-3)Galβ(1-4)Glc-18:0

(4) Enzymatic basis of branching and extension of O-Man glycans for keratan sulfate biosynthesisJournal of Biological ChemistryFebruary 1, 2026Tomoya Itoh, Hide-Nori Tanaka, Mohit Pareek, Masamichi Nagae, Hiroshi Manya, Akemi Ido, Sushil K. Mishra, Yasuhiko Kizukafor 5 min. For sialidase treatment, Clostridium perfringens α2-3,6,8 sialidase (New England Biolabs, P0720) was added simultaneously with PNGaseF. For endo-β-galactosidase treatment, the ChABC-treated samples

(5) EatA mediated degradation of intestinal mucus is species-specific and driven by MUC2 structural featuresNature CommunicationsDecember 31, 2025Sergio Trillo-Muyo, Brendan Dolan, Frida Svensson, Tim J. Vickers, Liisa Arike, Maria-Jose García-Bonete, Jenny K. Gustafsson, Mathias I. Nielsen, Hans H. Wandall, James M. Fleckenstein, et al.analysis MUC2C was deglycosylated with β1–3 Galactosidase (P0726, New England Biolabs), α2-3,6,8 Neuraminidase (P0720, New England Biolabs) and O-glycosidase (P0733, New England Biolabs) either as a single reaction or

(6) Sialoglycoconjugate profiling of human choroid, retinal pigment epithelium, and basal laminar depositsExperimental Eye ResearchNovember 1, 2025Emma M. Navratil, Piper A. Wenzel, Miles J. Flamme-Wiese, Jack E.B. B. Miller, Luke A. Wiley, Edwin M. Stone, Budd A. Tucker, Robert F. Mullinssections were incubated with α-2-3,6,8 neuraminidase in 1x GlycoBuffer or 1x GlycoBuffer only (NEB #P0720L, Ipswich, MA) for 16 hours at 37C (n=5–8). Enzyme treated sections received 200 units of neuraminidase

(7) Conversion of an agonistic anti-TNFR2 biparatopic antibody into an antagonist by insertion of peptide linkers into the hinge regionJournal of Biological ChemistrySeptember 1, 2025Takuya Otsuki, Shigeyuki Matsumoto, Junso Fujita, Tomoko Miyata, Keiichi Namba, Ryo Kanada, Yasushi Okuno, Haruhiko Kamada, Hiroaki Ohno, Hiroki Akibaaffinity chromatography, was mixed with 2 μl O-glycosidase (#P0733S, New England BioLabs), 2 μl α2-3,6,8 neuraminidase (#P0720S, New England BioLabs), 15 μl of 10 × GlycoBuffer 2 (provided with O-glycosidase), and deionized-distilled

(8) Lysosome-mediated aggregation of galactose-deficient IgA1 with transferrin receptor 1 links to IgA nephropathyNat CommunJuly 1, 2025Meijun Si, Jingpeng Fu, Mengting Fang, Yunfei Lu, Junxuan Huang, Haojie Li, Peiyi Wang, Maofu Liao, Jian Zhu, Peiyao Li, et al.generating Gd-IgA118,28–31. 1 mg of purified IgA1 proteins were treated with 4000 U α2-3,6,8 neuraminidase (P0720L; New England Biolabs), and 400 U β1-3 galactosidase (P0726S; New England Biolabs) in PBS, pH 7.4, at

(9) Structural and Functional Characterization of the 28 kDa Structured Core of BmSA1, the Major Surface Antigen of Babesia MicrotiProteins: Structure, Function, and BioinformaticsMay 9, 2025Assia Mouhand, Joana Pissarra, Philippe Barthe, Christian Roumestand, Stéphane DelbecqRBCs. For the enzymatic treatment, 50 μL of packed RBCs were incubated with α2‐3,6,8 Neuraminidase (P0720, New England BioLabs, France) at 5 U/mL, or Trypsin (from Proti‐Ace kit, Hampton Research, USA) at a

(10) A Tissue Section-Based Mid-Infrared Spectroscopical Analysis of Salivary Gland Tumors Based on Enzymatic DeglycosylationCancers (Basel)May 1, 2025Julie Wellens, Robin Vanroose, Sander De Bruyne, Hubert Vermeersch, Benjamin Denoiseux, David Creytens, Joris Delanghe, Marijn M. Speeckaert, Renaat Coopmanα2-6-, and α2-8-linked sialic acid residues from glycoproteins and oligosaccharides (2000 units, ref. P0720S, New England Biolabs, Ipswich, MA, USA). Second, α1-2,4,6 fucosidase O was used to catalyze the hydrolysis

(11) Profiling the regulatory landscape of sialylation through miRNA targeting of CMP- sialic acid synthetaseJournal of Biological ChemistryApril 1, 2025Faezeh Jame-Chenarboo, Joseph N. Reyes, Thusini Uggalla Arachchige, Lara K. Mahalnegative control for lectin staining, untransfected cells were treated with neuraminidase (NEB, catalog #: P0720 L) prior to staining. For staining, 15 μg/ml of FITC-SNA (Vector Labs, cat. #: FL-1302–2) or Cy5-diCBM40

(12) Bisecting N-Acetylglucosamine of the N-Glycan of Immunoglobulin G Does Not Affect Binding to Fc Gamma ReceptorsACS Chemical BiologyMarch 21, 2025Gerlof P. Bosman, Inèz D. Stoof, Hans P. Bastiaansen, Linda Quarles van Ufford, Justyna M. Dobruchowska, Jan-Willem H. Langenbach, Bhargavi M. Boruah, Kelley W. Moremen, Arthur E. H. Bentlage, Suzanne N. Lissenberg-Thunnissen, et al.mg) was dissolved in 50 mM MOPS (pH 6.5, 3 mL) to which neuraminidase (C. perfringens, 40 μL, 1000 U, P0720, New England Biolabs) and galactosidase BgaA (300 μL, 1 mg mL–1) was added, and the reaction mixture

(13) Dual functional POGases from bacteria encompassing broader O-glycanase and adhesin activitiesNat CommunFebruary 25, 2025Linjiao Zhou, Uriel Ortega-Rodriguez, Matthew J. Flores, Yasuyuki Matsumoto, John Q. Bettinger, Wells W. Wu, Yaqin Zhang, Su-Ryun Kim, Thomas G. Biel, Jordan D. Pritts, et al.and 5% CO2. The next day, cells were washed with PBS and treated with 50 mU pan-neuraminidase (Cat# P0720L, New England Biolabs) in PBS for 2 h at 37 °C, then fixed with fixation buffer (Cat# 420801, BioLegend)

(14) A Novel Missense Variant of BMPR1A in Juvenile Polyposis Syndrome: Assessment of Structural and Functional AlternationsHuman MutationFebruary 18, 2025Mengyuan Yang, Ziyan Tong, Zhijun Yuan, Bingjing Jiang, Yingxin Zhao, Dong Xu, Ying Yuan(PNGase F, P0704S) to remove N-glycans or O-glycosidase (O-Gly) (P0733S) and α2-3,6,8 neuraminidase (P0720S) to remove O-glycans, and these enzymes are all purchased from New England Biolabs (Beverly, Massachusetts).

(15) The O-glycosyltransferase C1GALT1 promotes EWSR1::FLI1 expression and is a therapeutic target for Ewing sarcomaNat CommunFebruary 2, 2025Shahid Banday, Alok K. Mishra, Romana Rashid, Tianyi Ye, Amjad Ali, Junhui Li, Jason T. Yustein, Michelle A. Kelliher, Lihua Julie Zhu, Sara K. Deibler, et al.whole-cell lysates (600 μg) were pre-treated with 100 U α2-3,6,8 neuraminidase (New England BioLabs, Cat# P0720S) in the presence or absence of 800 U O-glycosidase (New England BioLabs, Cat# P0733S) for 2 h at room

(16) Alcohol induces α2-6sialo mucin O-glycans that kill U937 macrophages mediated by sialic acid-binding immunoglobulin-like lectin 7 (Siglec 7)FEBS open bioJanuary 1, 2025Cheng PW, Hothpet VR, Bhat G, Bailey K, Li L, Samuelson DR.Abs‐mouse IgG (Cat no. 31188), Fisher Scientific, Pittsburgh, PA; Nonspecific neuraminidase (Cat no. P0720), New England Biolabs, Ipswich, MA; Bronchial epithelial growth medium, (Cat no. SC3211b) Lonza, Basel,

(17) Glycosylation changes of vWF in circulating extracellular vesicles to predict depressionScientific reportsNovember 23, 2024Yamada N, Tominaga K, Tominaga N, Kobayashi A, Niino C, Miyagi Y, Yamagata H, Nakagawa S.treatment in vitro for plasma EVs Plasma EVs (400 ng) of HCs were treated with α2–3,6,8 Neuraminidase (P0720, New England BioLabs, MA, USA) at least 50 units per sample at 37 °C for 1 h. The removal of glycans

(18) A Deficiency in Glutamine-Fructose-6-Phosphate Transaminase 1 (Gfpt1) in Skeletal Muscle Results in Reduced Glycosylation of the Delta Subunit of the Nicotinic Acetylcholine Receptor (AChRδ)BiomoleculesOctober 3, 2024Stephen Henry Holland, Ricardo Carmona-Martinez, Kaela O’Connor, Daniel O’Neil, Andreas Roos, Sally Spendiff, Hanns Lochmüller(New England Biolabs, #P0704S, Whitby, ON, Canada) or α2-3, 6, 8 Neuraminidase (New England Biolabs, #P0720S) according to manufacturer’s instructions. AChRδ protein levels and molecular weight were assessed via

(19) The modified RNA base acp3U is an attachment site for N-glycans in glycoRNACellSeptember 19, 2024Yixuan Xie, Peiyuan Chai, Nicholas A. Till, Helena Hemberger, Charlotta G. Lebedenko, Jennifer Porat, Christopher P. Watkins, Reese M. Caldwell, Benson M. George, Jonathan Perr, et al.mix was a final volume of 250 μL in 1x NEB Glyco Buffer #1 with 5 μL of α2–3,6,8 Neuraminidase (NEB, P0720) and 5 μL of α2–3,6,8,9 Neuraminidase A (NEB, P0722). PNGaseF digestion mix was a final volume of 250

(20) O-glycosylation contributes to mammalian glycoRNA biogenesisbioRxivAugust 31, 2024Jennifer Porat, Christopher P. Watkins, Chunsheng Jin, Xixuan Xie, Xiao Tan, Charlotta G. Lebedenko, Helena Hemberger, Woojung Shin, Peiyuan Chai, James J. Collins, et al.sialidase treatment, a 10 uL reaction is performed using 2.5 U of α2-3,6,8 neuraminidase (NEB; Cat. No. P0720L) and 1 U of α2-3,6,8,9 neuraminidase (NEB; Cat. No. P0722L), 50 mM phosphate buffer, pH 6.0, and small

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3/13/2019Works to Cleave Alpha 2-3 Sialylations, but Is Not Very Efficient Mark EWorks moderately, but not great for sensitive life science applications.

a2-3,6,8 Neuraminidase

a2-3,6,8 Neuraminidase from New England Biolabs

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New England Biolabs

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a2-3,6,8 Neuraminidase from New England Biolabs

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