Fig 1: Expression of AXL in parental and trastuzumab-resistant breast cancer cells. (A) Differential expression of AXL expression in trastuzumab-resistant SKBR3 and HCC1954 cells as determined by qPCR. (B) in the presence or absence of the ligand, GAS6, (C) in the presence and absence of hedgehog inhibitor, GANT61 and GAS6. Two-way ANOVA followed by Tukey’s multiple comparisons post hoc test was used to determine statistical significance. Parental (P) and resistant (R) cell lines are indicated. Data are presented as mean ± SD (n = 3). * p ≤ 0.05.
Fig 2: AXL expression regulates stemness independently of GAS6 in both parental and resistant SKBR3 and HCC1954 cell lines. Stemness-related gene expression: (A,B) In the presence of GAS6 in SKBR3 parental and resistant cells. (C,D) In the absence of GAS6 in SKBR3 parental and resistant cells. (E,F) In the presence of GAS6 in HCC1954 parental and resistant cells. (G,H) In the absence of GAS6 in HCC1954 parental and resistant cells. Two-way ANOVA followed by Tukey’s multiple comparisons post hoc test was used to determine statistical significance. Parental (P) and resistant (R) cell lines are indicated. Data are presented as mean ± SD (n = 3). * p ≤ 0.05.
Supplier Page from Abcam for Recombinant Human GAS 6 protein (GST tag N-Terminus)