Fig 1: Karyoplot analysis of native CpA in control HeLa cells, versus in the presence of CpA-TAP, and versus in the presence of CpA-TAP + DAXX KO.
Fig 2: ChIPseq analysis of native CpA-TAP in DAXX KO HeLa cells. a) Western confirmation that DAXX is knock-out and that CpA-TAP is esxpressed (rDAXX: recombinant DAXX protein, AbCam cat #ab131785). b) CoIF of CpA-TAP and native CENP-C during interphase and mitosis (left panel), and native CpA versus S-tag ChIP followed by CENP-C Western (right panel). Scale bar = 5 μm. c) Venn diagram detailing total, centromeric, and non-centromeric hotspots for native CpA and CpA-TAP. d) Categorical dissection of native CpA versus CpA-TAP incorporated sites
Fig 3: Karyoplot analysis of native CpA versus CpA-TAP deposition in both control (untransfected) HeLa and DAXX KO cells
Supplier Page from Abcam for Recombinant Human Daxx protein (GST tag N-Terminus)