Fig 2: Comparative analysis of endoplasmic reticulum (ER) chaperone gene transcription in the adipose tissue, oviduct magnum of wild-type (WT) and ADPN KI hens, and HEK293 cells(A−D) mRNA expression levels of Ero1-Lα, DsbA-L, ERP44, and PDI in the adipose tissue and oviduct magnum of WT and ADPN KI hens compared with HEK293 cells. Samples from WT hens are denoted as WT adipose tissue and WT oviduct magnum, while samples from ADPN KI hens are labeled as ADPN adipose tissue and ADPN oviduct magnum. Statistical analysis was performed using one-way ANOVA (n = 3). Different superscript letters indicate significant differences between groups (p < 0.05)

Fig 3: Comparative analysis of the multimeric composition of recombinant hADPN(A) Detection of multimeric recombinant hADPN derived from OVA ADPN KI chicken egg white (EW)-derived hADPN and other commercial recombinant hADPNs derived from human embryonic kidney (HEK) 293 cells with a C-terminal FLAG tag, High-Five (Hi-5) cells, and human serum (positive control) by western blotting analysis under non-reducing condition. (B) Detection of reduced EW-derived recombinant hADPN and other commercial recombinant hADPNs derived from HEK293 cells, Hi-5 cells, and human serum by western blotting analysis. Arrows indicate HMW, hexamer (medium), trimer (low), and monomer forms of hADPN.