Fig 1: Effect of high glucose induction on FSTL1 expression in HK-2 cells. (A, B) Effect of high glucose concentration on FSTL1 expression in HK-2 cells. (A) FSTL1 protein expression (Western blotting); (B) FSTL1 mRNA expression (qPCR). (C, D) Effect of high glucose duration of action on FSTL1 expression in HK-2 cells. (C) FSTL1 protein expression (Western blotting); (D) FSTL1 mRNA expression (qPCR). (E, F) Effect of osmotic pressure on FSTL1 expression in HK-2 cells. (E) FSTL1 protein expression (Western blotting); (F) FSTL1 mRNA expression (qPCR). *P < 0.05 and **P < 0.01. NG, normal glucose; HG, high glucose; Mannitol, high osmolarity.
Fig 2: Effect of FSTL1 on oxidative stress in HK-2 cells after blocking the NF-κB signaling pathway. (A, B, C) Silencing FSTL1 expression. (A) GSH level; (B) SOD level; (C) MDA level. (D, E, F) Giving rhFSTL1. (D) GSH level; (E) SOD level; (F) MDA level. Compared to the LV-shFSTL1 + HG group, *P < 0.05 and **P < 0.01. Compared to the rhFSTL1 + HG group, ##P < 0.01.
Fig 3: Effect of FSTL1 on cell migration in HK-2 cells after blocking the NF-κB signaling pathway. (A) Silencing FSTL1 expression; (B) Giving rhFSTL1. Compared to the LV-shFSTL1 + HG group, **P < 0.01. Compared to the rhFSTL1 + HG group, #P < 0.05.
Fig 4: Effect of FSTL1 on transdifferentiation of high glucose-induced HK-2 cells. (A) Silencing FSTL1 expression; (B) Giving rhFSTL1. Compared to the NG group, *P < 0.05 and **P < 0.01. Compared to the HG group, #P < 0.05 and ##P < 0.01.
Fig 5: Effect of FSTL1 on HK-2 cell proliferation after blocking the NF-κB signaling pathway. (A, C) CCK-8 assays for cell proliferation. (B, D) Flow cytometry assays for cell proliferation. Compared to the LV-shFSTL1 + HG group, *P < 0.05. Compared to the rhFSTL1 + HG group, #P < 0.05. PDTC, NF-κB signaling pathway inhibitor.
Supplier Page from R&D Systems, a Bio-Techne Brand for Follistatin-like 1/FSTL1 Protein
Available conjugates: Sizes Available: 50 ug