Fig 1: NPL1010 and NPL3008 rarely inhibit BMP1-dependent COL3A1 cleavage. (A) COL3A1 cleavage assay. rhCOL3A1 was incubated with rhBMP1. The effects of NPL1010 and NPL3008 on BMP1-dependent rhCOL3A1 cleavage were analyzed using SDS–PAGE and Oriole staining. Arrows indicate full-length rhCOL3A1 and cleaved rhCOL3A1. (B) Statistical analysis of BMP1-dependent cleavage of COL3A1. The levels of noncleaved and cleaved COL3A1 were measured by performing densitometry of the Oriole-stained gel, and the percentage of cleaved COL3A1 was calculated based on the fragments detected at each molecular weight and background. **: p < 0.01 (Dunnett’s test). The error bar indicates +s.d. with individual data shown (n = 4).