Fig 1: The effect of CD27 on proliferation and apoptosis of myeloma cells. A, B CCK-8 was used to detect the proliferation of U266 and RPMI-8226 cells treated with sCD27 and 1F5 for 0, 24, 48, and 72 h. C, D Flow cytometry was used to analyze the apoptosis of U266 and RPMI-8226 cells treated with sCD27 and 1F5 for 0, 48, and 72 h. E qPCR detection of mRNA levels of PERK and ATF4 in MM cells treated with sCD27 and 1F5. F Western blotting detection of protein levels of PERK and ATF4 in MM cells treated with sCD27 and 1F5. G The correlation between ATF4 expression and OS of MM patients in GSE57317 was conducted by Kaplan–Meier analysis. * P < 0.05, ** P < 0.01, *** P < 0.001 and **** P < 0.0001
Fig 2: Enrichment analysis and PPI network of CD27 differentially expressed genes in MM. A Volcano map of CD27 differentially expressed genes in the GSE5900 and GSE6477. B Heatmap of the 50 differentially expressed genes. The color from Red to blue represents that the genes are from high to low. C GO enrichment analysis of DEGs. D KEGG enrichment analysis of DEGs. E PPI network of CD27
Fig 3: Correlation analysis of the microenvironment. A Flow cytometry detection of CD70 and PD-L1 expression in U266 and RPMI-8226 MM cells treated with sCD27 and 1F5. B Bar plot showing the ratio of 22 immune cell types in GSE5900 and GSE6477 samples. C Box line plots are used to represent the 22 immune cell types in the low and high CD27 patient cohorts in blue and red, respectively. D Comparison of ssGSEA for representation of 28 immune cell expressions in low and high CD27 patient cohorts. E Significantly enriched NK-κ B signaling pathway in GSEA. * P < 0.05, ** P < 0.01, *** P < 0.001 and **** P < 0.0001
Fig 4: The expression level of CD27 in MM patients with different clinical characteristics. A, B CD27 expression level in different subtypes of myeloma patients in GSE5900 and GSE6477. C The expression level of CD27 in different recurrence states of MM in GSE31161. D Comparison of CD27 expression levels between ISS I/II and ISS III phases in GSE136337. E, F Expression of CD27 in different survival subgroups (EFS24m and OS24m) in GSE24080. * P < 0.05, ** P < 0.01, *** P < 0.001 and **** P < 0.0001
Supplier Page from Abcam for Recombinant Human CD27 protein (GST tag N-Terminus)