Fig 1: Blood vessel growth on the avascular corneas of mice after suppression of gene expression by using an ANGPTL7 PshRNA. ANGPTL7 mRNA expression was inhibited by almost 80% inhibition at 48 h after intrastomal injection of the ANGPTL7 PshRNA, but normal expression was restored by 150 h (A, n = 3 per condition). The corneas of mice injected intrastromally with the control PshRNA did not exhibit any expression change (B, left panel). Notably, intrastromal injection of the ANGPTL7 PshRNA resulted in the spontaneous development of blood vessel growth from the limbal areas in the avascular mouse corneas by day 4 after the injection (B, right panel). Areas of vessels in the mouse corneas were significantly different following the injection of control PshRNA injection and ANGPTL7 PshRNA injection (p < 0.001, C, n = 4 per condition)
Fig 2: Relative expression of ANGPTL7 in HCKs and HDFs. AKR1B10, GRP, ANGPTL7, and PIP levels were elevated in the cDNA derived from HCKs as compared to with that from HDFs (A). In western blot analysis, ANGPTL7 was detected in culture media collected from HCKs but not i HDFs (B). N = 3 per condition.
Fig 3: ANGPTL7 siRNA tranfection increased tube formation in HCK-HUVEC co-culture. ANGPTL7 mRNA expression was drastically inhibited following the administration of ANGPTL7 siRNA for up to 240 h after transfection (A). The tube-formation areas under the normal serum-free medium (normal) and control siRNA treatment were 0.13 ± 0.15% and 0.31 ± 0.09%, respectively (B). In the cells transfected with the ANGPTL7 siRNA the tube formation areas increased significantly (1.41 ± 0.54%, p < 0.001, B). This siRNA effect was potently suppressed when human recombinant ANGPTL7 (A7 siRNA+rhANGPTL7) was added to the cells (0.69 ± 0.30%, p < 0.001, B). N = 12 per condition. In the cells in which ANGPTL7 expression was suppressed, the mRNA expression levels of ANGPTL1 and ANGPTL4 were also decreased (by 48.4% and 56.1%, respectively), but the expression of ANGPTL2 was increased (119%). N = 2 per each condition.
Supplier Page from R&D Systems, a Bio-Techne Brand for Angiopoietin-like Protein 7/ANGPTL7 Protein
Available conjugates: Sizes Available: 25 ug