Fig 1: Impact of high dietary Na+ intake on expression of phosphorylated STE20/SPS1-related proline alanine-rich kinase (SPAK) and oxidative stress response 1 (OxSR1) expression in male and female Sprague-Dawley (SD), Dahl salt-resistant (DSR), and Dahl salt-sensitive (DSS) rats. Total pSPAK (S373)/pOxSR1 (S325) expression and pOxSR1 (T185) expression SPAK and OxSR1 protein expression expressed as fold change and representative immunoblots in 3-mo-old male and female SD (A), DSR (B), and DSS (C) rats fed a 21-day normal salt (NS; 0.6% NaCl) or high-salt (HS; 4% NaCl) diet are shown. Values are means ± SD; n = 6 animals per group. Protein expression is shown as optical density units (ODU)/mm2 normalized to total protein. Differences in protein expression within each group between NS and HS diets were determined using a Student’s t test. Differences in protein expression between groups (male vs. female) were determined using two-way ANOVA, and a post hoc test with Dunn–Šidák correction was used to evaluate variation among groups. *P < 0.05 vs. the respective NS group. #P < 0.05 vs. the respective male group.
Fig 2: Impact of high dietary Na+ intake on STE20/SPS1-related proline alanine-rich kinase (SPAK) and oxidative stress response 1 (OxSR1) expression in male and female Sprague-Dawley (SD), Dahl salt-resistant (DSR), and Dahl salt-sensitive (DSS) rats. Total SPAK and OxSR1 protein expression expressed as fold change and representative immunoblots in 3-mo-old male and female SD (A), DSR (B), and DSS (C) rats fed a 21-day normal salt (NS; 0.6% NaCl) or high-salt (HS; 4% NaCl) diet are shown. Values are means ± SD; n = 6 animals per group. Protein expression is shown as optical density units (ODU)/mm2 normalized to total protein. Differences in protein expression within each group between NS and HS diets were determined using a Student’s t test. Differences in protein expression between groups (male vs. female) were determined using two-way ANOVA, and a post hoc test with Dunn–Šidák correction was used to evaluate variation among groups. *P < 0.05 vs. the respective NS group. #P < 0.05 vs. the respective male group.
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