Fig 1: Alpha-fetoprotein (AFP) antigen levels detected and the standard curve chart by the AFP protein microarray. The standard curve of AFP was created by applying different concentrations of AFP antigen (1: 80 ng/ml, 2: 40 ng/ml, 3: 20 ng/ml, 4: 10 ng/ml, 5: 5 ng/ml, 6: 2.5 ng/ml, 7: 1.25 ng/ml, 8: 0.0625 ng/ml, and 9: 0.03125 ng/ml) and 10: 0.05% phosphate-buffered saline-Tween 20 as a negative control to the microarray plate coated with the anti-AFP antibody. The colour version of this figure is available at: http://imr.sagepub.com.
Fig 2: Immunostains for intrinsic hepatic and breast cancer markers (A) Intrinsic liver marker AFP. Serum AFP by ELISA was below the limit of detection (data not shown). (B) Liver marker Glypican-3. (C) Liver marker Hep Par 1. (D) Cancer marker HER2/Neu. (E) Cancer marker P53. (F) Cancer marker Ki-67 (nuclear).
Fig 3: Scan chart of HCC serum and normal healthy serum samples detected by the AFP/LCA protein microarray assay.HCC serum samples, 1–9; normal healthy serum sample, 10.
Fig 4: (A) Scan chart of AFP-L3 standard substances detected by the AFP/LCA-coated chips. AFP antibodies and LCA were applied to the slides. a: AFP antibody, 0.5 mg/ml; b: LCA, 4 mg/ml. Serum samples with different concentrations of AFP-L3 were used: (1–5) 100, 50, 25, 12.5, and 6.25 ng/ml; (6–9) 100, 50, 25, and 12.5 ng/ml; (10) blank control. (B) The standard curve chart and the regression equation of AFP-L3 determined using the AFP/LCA protein microarray assay.
Fig 5: (A) AFP antigen levels detected by the AFP protein microarray. AFP antibodies were applied to the slides at different concentrations: a, 1 mg/ml; b, 0.5 mg/ml; c, 0.25 mg/ml; and d, 0.125 mg/ml. Different concentrations of the AFP antigen were used: 1.80 ng/ml, 2.40 ng/ml, 3.20 ng/ml, 4.10 ng/ml, and 5.5 ng/ml. Sera from HCC patients and healthy controls were also included: 6, HCC serum; 7, HCC serum; 8, blank control; 9, healthy serum; and 10, HCC serum. (B) The standard curve chart and the regression equation for determining the AFP levels using the AFP protein microarray assay.
Supplier Page from Abcam for Human alpha Fetoprotein ELISA Kit (AFP)