Fig 1: Intracellular iron ion regulating mediated PpIX accumulation and DNA repair enzyme inhibition. (A) The membrance fusion of CLs@DiO and MFLs@DiO (scale bar = 10 μm), the distribution of MFLs@DiI in B16–F10 cells (B) (scale bar = 10 μm). (C) The detection of Fe2+ after incubating with B16–F10 cells for 4 h (scale bar = 25 μm). (D, E) The transformation of 5-ALA analyzed after incubating with B16–F10 cells for 4 h by CLSM (scale bar = 10 μm) and flow cytometry, respectively. (F) DFO inhibits ALKBH2 repair of m1A in dsDNA by using the DpnII digestion assay and semi-quantitative analysis (G), 1: maker, 2: dsDNA (m1A), 3: dsDNA (m1A)+DpnII, 4: dsDNA (m1A)+ALKBH2+Fe2++DFO + DpnII, 5: dsDNA (m1A)+ALKBH2+Fe2++DpnII. Data are presented as means ± SD (n = 3). ∗∗∗∗P < 0.0001, ∗∗P < 0.01, ∗P < 0.05.