Endo H from New England Biolabs

Product Specs
ItemEndo H
CompanyNew England Biolabs
Price $326.00 Inquire
Catalog NumberP0702L
Molecule NameEndo H
Source/Expression SystemRecombinant
ApplicationsProteomics & Glycomics
Concentration500,000 units/ml
Quantity50000 units
Temperature-20 C
TypeGlycosidases

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Design of an oral vaccine against Salmonella pullorum using protein surface display on bioengineered Saccharomyces cerevisiaeVeterinary QuarterlyDecember 31, 2026Qian He, Mei Zheng, Xiaojing Zhang, Fuhao Li, Meilin Qin, Zhiyi Yang, Xiaoping Liao, Jian Sun, Hao Ren, Yang Yudeglycosylation of the extracts was performed according to the manual instructions of Endoglycosidase H (Endo H) (P0702S, New England Biolabs). The mixture digested by Endo H was stored at −20 °C until further characterization.

(2) Structural basis of GAIN domain autoproteolysis and cleavage-resistance in the adhesion G-protein coupled receptorsNat CommunApril 6, 2026Fabian Pohl, Florian Seufert, Yin Kwan Chung, Robin Schick, Björn Kieslich, Torsten Schöneberg, Tobias Langenhan, Peter W. Hildebrand, Norbert Sträterreceptors, 17 μl of the lysates was treated with 500 U PNGase F (NEB, cat# P0704S), 500 U Endo H (NEB, cat# P0702S) or distilled water (1 μl each), supplemented with 2 μl of their respective 10x commercial buffer. The

(3) Biochemical analysis of a TrkB receptor mutation that causes a developmental epileptic encephalopathyJournal of Biological ChemistryMarch 1, 2026Yang Zhong Huang, Cormic McNamara, James O. McNamara100˚C for 10 min. Denatured proteins were digested with PNGase F (catalog #P0704) or Endo H (catalog #P0702) (1000 units) at 37 °C for 60 min. The extent of deglycosylation was assessed by mobility shifts on

(4) SEL1L-HRD1 ER-associated degradation facilitates prohormone convertase 2 maturation and glucagon production in islet α cellsNature CommunicationsFebruary 25, 2026Wenzhen Zhu, Linxiu Pan, Xianwei Cui, Anna Chiara Russo, Rohit Ray, Brent Pederson, Xiaoqiong Wei, Liangguang Leo Lin, Mauricio Torres, Hannah Hafner, et al.treated with cycloheximide (50 µg/ml; Sigma-Aldrich C7698), endoglycosidase H (New England Biolabs P0702S), or PNGase F (New England Biolabs P0704S) per manufacturer’s instructions. In siRNA treatment experiments,

(5) VAMP7-dependent late endosomal secretion of ER and mitochondrial proteins impacts the tumor microenvironment and macrophage engagementNature CommunicationsFebruary 21, 2026Somya Vats, Pedro Dionisio, Quentin Lemercier, Raphael Pineau, Ludivine Therreau, Joanna Lipecka, Béatrice Cholley, Jean-Baptiste Moog, Jose Wojnacki, Céline Keime, et al.co-transfected with the Myc-RTN3A and GFP-CD63 plasmids, beads were treated with Endo H (New England Biolabs, P0702L) or Rapid PNGase F (New England Biolabs, P0710S) following the protocols of the manufacturer. For Endo H

(6) Identification of a novel apoB variant in a family exhibiting hypocholesterolemia: Mechanistic insightsJournal of Clinical LipidologyFebruary 20, 2026Rachid Essalamani, Alexandra Evagelidis, Anna Roubtsova, Michael Chong, Nazia Pathan, Guillaume Pare, Evgeniy V. Petrochenko, Mart Reimund, Alan T. Remaley, Lorraine Chalifour, et al.digestions Proteins (30 to 50 μg) were digested for 90 min at 37°C with endoglycosidase H (endo H; P0702L) or endoglycosidase F (endo F; P0705S) as recommended by the manufacturer (New England BioLabs, ON,

(7) RIC-3 Interacts Directly with the 5-HT 3A Receptor to Mediate Trafficking Across Subcellular CompartmentsbioRxivFebruary 18, 2026Nermina Jahovic, Petar N. Grozdanov, Rhea Ramani, Cade Perkins, Clinton C. MacDonald, Joshua Theriot, Quynh Hoa Do, Michaela Jansenunder native conditions, glycoprotein samples were treated with PNGase F (NEB #P0708S) or Endo H (NEB #P0702S) following the manufacturer’s non-denaturing protocols. 2.8. α7 and 5-HT3A surface expression following

(8) Generation and characterization of human iPSC-derived NPC1 I1061T/I10161T i 3 Neurons as a model for NPC1 diseasebioRxivFebruary 17, 2026Shikha Salhotra, Niamh X. Cawley, Christian White, Insung Kang, Anika Prabhu, Cristin D. Davidson, Christopher A. Wassif, Forbes D. Porteri3Neurons with and without the respective enzymes. For both Endo H (New England Biolabs, Ipswich, MA, #P0702S) and PNGase F (New England Biolabs, #P0704S) treatment, 100 μg of total protein for each cell type was

(9) Transgenic mouse-derived human monoclonal antibodies targeting EBV gp350 and gp42 provide basis for therapeutic developmentCell Reports MedicineFebruary 17, 2026Crystal B. Chhan, Kevin Lang, Amelia R. Davis, Yu-Hsin Wan, Nicholas T. Aldridge, Gargi Kher, Samuel C. Scharffenberger, Samantha R. Hardy, Roman Iureniev, Natalia V. Giltiay, et al.BioTechne,Cat#6748-CD GeneJuice Tranfection Reagent,SigmaAldrich,Cat# 70967 Endoglycosidase H,New England Biolabs,Cat#P0702S "Formvar, stabilized with Carbon-coated 400-mesh copper grids",Ted Pella,Cat#01754-F Uranyl Formate,Electron

(10) Alpha-methyl-para-tyrosine and amphetamine ameliorate hyperactivity in a novel mouse model of dopamine transporter deficiency syndromeEMBO Molecular MedicineFebruary 16, 2026Emma E Russo, Ameneh Rezayof, Conner Wallace, Erin Q Williams, Pieter Beerepoot, Marija Milenkovic, Maria Novalen, Aled Blundell, Tatiana V Lipina, Jason Locke, et al.glycosidases peptide N-glycosidase F (PNGase F) and EndoH (New England Biolabs, catalog #P0704S and #P0702S, respectively). Sample proteins were then separated by 10% SDS-PAGE and transferred to PVDF membranes.

(11) BIP orchestrates bidirectional ER protein trafficking via co-chaperone complexesCellular & Molecular Biology LettersFebruary 14, 2026Suma Biadsy, Ayelet Gilad, Laila Abu Madegam, Aeid IgbariaSDS–PAGE. Endoglycosidase H (Endo H) assay Endo H was purchased from New England Biolabs (NEB; no. P0702L). Following immunoprecipitation, total protein lysates or co-immunoprecipitation eluates were denatured

(12) Differential regulation of translational stress responses by herpesvirus ubiquitin deconjugasesThe FEBS JournalFebruary 1, 2026Jiangnan Liu, Noemi Nagy, Carlos Mario Ayala‐Torres, Maria G. MasucciSubstrate,"Thermo Scientific (Waltham, MA, USA)",Cat#XE356732 Endo H,"New England Biolabs (Ipswich, MA, USA)",Cat# P0702S Medium and buffer,Medium and buffer,Medium and buffer DMEM,Sigma‐Aldrich,Cat# D6429 EBSS,"Gibco (Waltham,

(13) Targeted delivery of rhodopsin’s assembled core is required for outer segment extension in mouse rod photoreceptorsJournal of Biological ChemistryFebruary 1, 2026Jorge Y. Martínez-Márquez, Sandy Hua, Andreea M. Beu, Christopher B. Stein, Jillian N. PearringH2O as necessary to a final volume of 20 μl. The reaction was incubated for 1 h at 37 °C. For endo H (P0702L, New England Biolabs) treatment, the same procedure was followed, except that GlycoBuffer 3 was used

(14) Sec61β maintains cytoplasmic proteostasis via ARIH1-mediated translational repression upon ER stressEMBO ReportsJanuary 27, 2026Hisae Kadowaki, Tomohisa Hatta, Kazuma Sugiyama, Tomohiro Fukaya, Takao Fujisawa, Takashi Hamano, Naoya Murao, Yasunari Takami, Shuya Mitoma, Tohru Natsume, et al.0.5% SDS and 40 mM DTT at 98 °C for 10 min and with Endoglycosidase H (Endo-H, New England Biolabs, P0702) at 37 °C for 1 h. The reaction was stopped with the addition of SDS-PAGE sample buffer. Pulse labeling

(15) Characterization of humoral and cellular immunity induced by mRNA vaccines expressing norovirus VP1 proteins in mice and NHPsMolecular TherapyJanuary 7, 2026Jiajie Wei, Karin Bystol, Leah Alabanza, Shuhui Lim, Jamie Greig, Pooja Gopal, Judith M. Reyes Ballista, Jennifer D. Galli, Christopher Warren, Andrew Swartz, et al.cooled, and two 40-μL aliquots were taken. One was treated with 500 U (1 μL) of Endo H (NEB, catalog no. P0702) for 1 h at 37°C; the other received 1 μL sterile water and was incubated as above. After Endo H treatment,

(16) Distinct regulatory elements of SLC6A14 expression contribute to modification of cystic fibrosis phenotypesHum GenetJanuary 5, 2026Mohsen Esmaeili, Cheng Wang, Fan Lin, Naim Panjwani, Adele Chan, Gengming He, Katherine Keenan, Julie Avolio, Ann Harris, Johanna M. Rommens, et al.For deglycosylation experiments, the samples were treated with PNGase F (NEB, P0704) or Endo H (NEB, P0702) according to the supplier's protocol except that denaturation by heating was replaced with incubation

(17) Interferon-inducible guanylate-binding protein 5 inhibits replication of multiple viruses by binding to the oligosaccharyltransferase complex and inhibiting glycoprotein maturationmBioDecember 10, 2025Shaobo Wang, Wanyu Li, Jing Wen, Lingling Wang, Shashi Kant Tiwari, William Bray, Lujing Wu, Na Li, Hui Hui, Alex E. Clark, et al.collected. Assessment of glycoprotein sensitivity to endoglycosidases Cell lysates were digested for 1 h with Endo H (P0702, NEB) or PNGase F (P0704, NEB) according to the manufacturer’s instructions. Upon completion of digestion,

(18) Antibodies disrupt bacterial adhesion by ligand mimicry and allosteric interferenceNature CommunicationsNovember 26, 2025Kelli L. Hvorecny, Gianluca Interlandi, Tim S. Veth, Pavel Aprikian, Anna Manchenko, Veronika L. Tchesnokova, Miles S. Dickinson, Joel D. Quispe, Nicholas M. Riley, Rachel E. Klevit, et al.and SDS-page quantification mAb deglycosylation was performed with Endoglycosidase H (Endo H, NEB #P0702) and Endoglycosidase S (Endo S, NEB #P0741) according to the manufacturer’s protocol. Briefly, 20 µg

(19) A Ciliary Phosphoinositide Pathway Regulates the Dosage of Polycystins in Primary CiliaJournal of the American Society of NephrologyNovember 25, 2025Chuan Chen, Zhifei Wang, Yue Gao, Madilyn R. Ellis, Biyun Ji, Cynthia J. Sieben, Courtney J. Haycraft, Mandy Croyle, Ariana Aghevli, Qingwen Xu, et al.minutes. The beads were divided into three equal parts, and two of them were treated using either Endo H (P0702L, NEB) or PNGase F (P0704L, NEB) kits following the manufacturer’s instructions. Then, all samples were

(20) Pathogenic variants at the N-terminal arginine residue 44 disrupt human GABA transporter 1 function: insights from Drosophila epilepsy modelsFrontiers in PharmacologyNovember 25, 2025Nikita Shah, Ameya Sanjay Kasture, Vasylyna Kovalchuk, Lara Bjeletic, Thomas Hummel, Harald H. Sitte, Sonja Sucic(cat. no. T1952-200UL). Endoglycosidase H was obtained from New England Biolabs GmbH (MA, USA; cat. no. P0702S). Tris and scintillation liquid (Rotiszint® eco plus) were provided by Carl Roth GmbH (Karlsruhe, Germany).

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New England Biolabs’ Endoglycosidase H (EndoH)
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Endo H

Endo H from New England Biolabs

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New England Biolabs’ Endoglycosidase H (EndoH)

Endo H from New England Biolabs

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