Fig 1: Fluorescence microscopy analysis of AC3 binding to SIRT1 enzyme and nuclei localisation. AC3-FAM (green-yellow), nuclei (DAPI, blue) and SIRT1 (Texas Red, red). Overlap of images can produced alterations in the colour results such as yellow produced by overlap of red and green, magenta produced by red and blue and cyan produced by green and blue. (A) A549, scale 100 µm. (B) HepG2 (augmentation in Fig. 20, ESI.† (C) Caco2, scale 200 µm). (D) U2OS, scale 200 µm. (E) MCF7, scale 20 µm. (F) MCF7 zoomed. (G) MDAMB-468, scale 100 µm. (H) Beas2B, scale 200 µm. Fluorescence intensity was measured by LUMIStar luminometer microplate readers as: green ex. 495 nm, em. 509 nm; blue ex. 359 nm, em. 461 nm and red ex. 596 nm, em. 615 nm. The results represent the mean ± SEM of two different experiments performed twice. Images were taken at a magnification of 100× in a Cytation 3.