Trypsin (0.25%), phenol red 15050057 from Thermo Fisher Scientific

Product Specs
ItemTrypsin (0.25%), phenol red
CompanyThermo Fisher Scientific
Price $37.50Supplier Page View Company Product Page
Catalog Number15050057
Conjugate/TagUnconjugated
Format0.25%
1X
Liquid
Molecule NameTrypsin
Size500mL
ApplicationsCELL CULTURE
Regulatory StatusFor Research Use Only. Not for use in diagnostic procedures

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Thermo Fisher Scientific

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Waltham, MA 02451

United States

Phone: 800-678-5599

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Website: www.thermofisher.com

(1) Suppression of haptoglobin and loss of striatal neurons in mice chronically exposed to chlorpyrifos contaminated drinking waterToxicological SciencesMarch 2, 2026Emma Fikse, Faith Anderson, Soyeon Cho, Jessica Landry, Elisa Carloni, Karl Biggs, Kathleen Paul, Thomas Daley, T Y Chang, Arminja Kettenbach, et al.Briefly, brains from neonatal mice (P0 to P3) were dissociated using 0.25% trypsin without EDTA (Gibco, 15050057) and repeated trituration using a pipette. Brain homogenate was plated on poly-L-lysine (Millipore Sigma,

(2) Group 1 mGluR stimulation rescues APOE4-mediated translation defects in neuronsLife Science AllianceFebruary 1, 2026Bindushree K Radhakrishna, Ahamed P Kaladiyil, Anushree Chakraborty, Vini Gautam, Ravi S MuddashettyRamakrishna et al, 2024). Briefly, the cortices were trypsinized for 5 min at 37°C using 0.25% trypsin (15050057; Thermo Fisher Scientific). After dissociation, the cells were plated onto cell culture plates containing

(3) TP53-dependent antitumor effects of DHODH Inhibition in nasopharyngeal carcinomaDiscover OncologyNovember 30, 2025Xingchen Dong, Yaoting Zhang, Zhicun Zhang, Ting Liu, Dongsheng Gu(Shanghai WheLab Bioscience, MC1280). For all cell passages, trypsin (0.25%) containing EDTA (Gibco, 15050057) was used for digestion. All cells were cultured in a carbon dioxide incubator at 37℃ and 5% CO2. Drug

(4) Aryl hydrocarbon receptor deficiency enhances astrocyte sensitivity to LPS-induced inflammationFrontiers in Cellular NeuroscienceNovember 14, 2025Emmanuel Ojo, Shelley A. Tischkaureaching approximately 90% confluence, adherent astrocytes were detached using Trypsin (0.25%) (Cat: 15050057; Gibco, United States) and subsequently replated, maintaining them in serum-free media for an additional

(5) Bat sarbecovirus WIV1-CoV bears an adaptive mutation that alters spike dynamics and enhances ACE2 bindingPLOS PathogensOctober 16, 2025Alexandra L. Tse, Gorka Lasso, Jacob Berrigan, Jason S. McLellan, Kartik Chandran, Emily Happy Miller24 h post-transfection, cells were incubated with increasing concentrations of trypsin (Gibco, Cat# 15050057) for 1 h at 37°C. Trypsin was stopped by adding 10% FBS for 15 min at 37°C. Cells were harvested and

(6) Spatiotemporal Adaptations-Driven Dynamic Thra Activation Simulates a Skin Wound Healing ResponseAdvanced science (Weinheim, Baden-Wurttemberg, Germany)September 1, 2025Li Z, Tan J, Zhou C, Zhou S, Ye Y, Li X, Shen X, Xie T, Wang M, Jiang J, et al.newborn mice within 24 h of birth. The dorsal skin was floated overnight in a 0.25% trypsin solution (#15050057, Gibco, USA) at 4 °C to separate the dermis and epidermis. Epidermal cells were obtained by cutting,

(7) The first abdominal aortic aneurysm organoid model replicates complex microenvironment for in vitro disease studyTheranosticsAugust 16, 2025Jiaxuan Feng, Mingjie Rong, Yudong Sun, Guanglang Zhu, Guangkuo Wang, Jiping Liu, Chen Wang, Jian Zhang, Xiaochen Ma, Junyi Yan, et al.U/mL) (abs47014929; Absin, China), and Hyaluronidase (0.4%, 2 mg/mL), each dissolved in Trypsin (0.25%, 15050057; Gibco, USA). The PDO were incubated with this mixture for 1 h, rinsed twice with PBS, and then centrifuged

(8) Single-cell transcriptomics identifies PDGFRA+ progenitors orchestrating angiogenesis and periodontal tissue regenerationInt J Oral SciJuly 24, 2025Jianing Liu, Junxi He, Ziqi Zhang, Lu Liu, Yuan Cao, Xiaohui Zhang, Xinyue Cai, Xinyan Luo, Xiao Lei, Nan Zhang, et al.maintained in an EC medium (1001, ScienCell, USA). Cells were passaged using a 0.25% trypsin solution (15050057, Gibco, USA) as they reached 80%–90% confluence. Culture conditions were maintained at 37 °C in a 5%

(9) AhR regulation of amyloid beta-induced inflammation in astrocyte cellsFront Cell NeurosciJuly 8, 2025Emmanuel Ojo, Temitope Adu, Raheem F. H. AI Aameri, Shelley A. Tischkauachieving approximately 90% confluence, adherent astrocytes were detached using Trypsin (0.25%) (Cat: 15050057; Gibco, United States) and subsequently related, maintaining them in serum-free media for an additional

(10) Global mitophagy inhibition via BNIP3 ablation is not sufficient to alleviate skeletal muscle impairments in male and female tumor-bearing miceJournal of Applied PhysiologyJune 1, 2025Francielly Morena, Ana Regina Cabrera, Toby L. Chambers, Pieter J. Koopmans, Seongkyun Lim, Stavroula Tsitkanou, Sabin Khadgi, Calvin Peterson, Eleanor R. Schrems, Ruqaiza Muhyudin, et al.37°C and 5% CO2. Media was changed up to every 48 hours. Upon ~80% confluency, cells were trypsinized (15050057, ThermoFisher Scientific, USA), washed 2X with sterile PBS, separated via centrifugation, and resuspended

(11) Rhein-induced apoptosis in colorectal cancer cell lines: A mechanistic study of the myeloid differentiation primary response gene 88/toll-like receptor 4/nuclear factor kappa-B signaling pathwayCytojournalApril 1, 2025Xinglu Zheng, Xiaolan Zhang, Longfei Hu, Xixi Chen, Zhangshu Zhao, Liangliang MaoBio-Rad Laboratories, Hercules, USA). Scratch assay The HT-29 and SW480 cells digested with trypsin (15050057, GIBCO BRL, Grand Island, NY, USA) were seeded into a 24-well plate. Once the cells achieved approximately

(12) Positive autoregulation of Sox17 is necessary for gallbladder and extrahepatic biliary duct formationDevelopmentJanuary 15, 2025Linh T. Trinh, Ryan R. Finnel, Anna B. Osipovich, Jessica R. Musselman, Leesa L. Sampson, Christopher V. E. Wright, Mark A. Magnusonwere split 1:6-1:8 at ∼80% confluence using 0.25% trypsin (5 min at 37°C) (Thermo Fisher Scientific, 15050057). During differentiation, feeder MEFs were depleted from mESCs via trypsinization and the mixed cells

(13) YAP1 mediates the dimensional and chemical coordination of immunoregulation and therapy in extensively passaged mesenchymal stem cellsTheranosticsJanuary 1, 2025Du FY, Zhou F, Zhao N, Bao L, Hu CB, Lei J, Liu AQ, Gao YF, Bao LH, Ni H, et al.2D culture, MSCs were plated directly on TCP and were passaged using 2.5% trypsin (Gibco, USA, Cat# 15050057). For 3D preconditioning, MSCs started at P3 were seeded in Matrigel (Corning, USA, Cat# 356231) and

(14) FBXO47 regulates centromere pairing as key component of centromeric SCF E3 ligase in mouse spermatocytesCommunications biologySeptember 7, 2024Ma A, Yang Y, Cao L, Chen L, Zhang JV.centrifugation at 120 × g for 2 min was performed, and the pellet was re-suspended in 5 mL 0.25% Trypsin (Gibco, 15050057) with 1 mg/mL DNase I at 37 °C for 5 min with gentle shaking. After centrifugation at 300 × g for 5

(15) Androgen receptor-induced molecules and androgen contribute synergistically to male-predominance of hepatocellular carcinomaiScienceAugust 16, 2024Jiayi Zhao, Letian Fang, Rui Pu, Wenbin Liu, Shiliang Cai, Ruihua Wang, Yiwei Shi, Zheng Li, Zihan Zhang, Zishuai Li, et al.#BL20263 crystal violet staining solution,Bio-light,Cat #BL31211 0.25% trypsin without EDTA,Gibco,Cat #15050057 Dihydrotestosterone,selleck,Cat #S4757 Flutamide,Sigma-Aldrich,Cat #F9397-5G Estradiol,selleck,Cat

(16) Distinct calcium sources regulate temporal profiles of NMDAR and mGluR-mediated protein synthesisLife Science AllianceAugust 1, 2024Sarayu Ramakrishna, Bindushree K Radhakrishna, Ahamed P Kaladiyil, Nisa Manzoor Shah, Nimisha Basavaraju, Kristine K Freude, Reddy Peera Kommaddi, Ravi S Muddashettylaboratory (25, 30, 78). Briefly, the cortices were trypsinized for 5 min at 37°C using 0.25% trypsin (15050057; Thermo Fisher Scientific). The homogenized and dissociated cells were plated on cell culture dishes,

(17) The ischemia‐enhanced myocardial infarction protection‐related lncRNA protects against acute myocardial infarctionMedComm (2020)July 10, 2024Rongzhou Wu, Tingting Wu, Qiaoyu Wang, Youyang Shi, Qianqian Dong, Xing Rong, Meiting Chen, Zhiyu He, Yu Fu, Lei Liu, et al.MIPRL knockout mice. After dissection and mincing, hearts were incubated with 0.0125% trypsin (Gibco, 15050057) at 4°C overnight. Then, 1.5 mg/mL of collagenase II (Worthington, LS004176) was added to digest the

(18) Energy competition remodels the metabolic glucose landscape of psoriatic epidermal cellsTheranosticsMay 27, 2024Weiwei Liu, Jingwei Jiang, Zeming Li, Yang Xiao, Siyi Zhou, Dehuan Wang, Yi Zou, Tiantian Liu, Ke Li, Huan Liang, et al.previously published articles 27-30. Briefly, dorsal skins of newborn mice were floated in 0.25% trypsin (#15050057, Gibco, USA) at 4 °C within 24 h of birth and digested overnight to isolate dermal and epidermal cells.

(19) Establishing a 3D culture system for early organogenesis of monkey embryos ex vivo and single-cell transcriptome analysis of cultured embryosSTAR ProtocolsMarch 15, 2024Nianqin Sun, Honglian Shao, Youyue Zhang, Baiquan Ci, Hui Yao, Bing Bai, Tao Tan,Thermo Fisher Scientific,Cat#10828028 Geltrex,Gibco,Cat#A1413202 Matrigel hESC-qualified matrix,Corning,Cat#354277 0.25% Trypsin (1×),Gibco,Cat#15050057; CAS: 9002-07-7 Phosphate-buffered saline (PBS),VivaCell,Cat#C3580-0500 Bovine serum albumin (BSA),Sigma-Aldrich,Cat#B2064;

(20) FUS-mediated HypEVs: Neuroprotective effects against ischemic strokeBioactive materialsNovember 1, 2023Wu Y, Huang X, Tan Z, Zang J, Peng M, He N, Zhang T, Mai H, Xu A, Lu D.After treatment, cells from each group were enzymatically detached using EDTA-free trypsin (Cat No. 15050057, Gibco, USA), washed with PBS, and subjected to analysis using a BC Gallios flow cytometer (Beckman Coulter

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Trypsin (0.25%), phenol red from Thermo Fisher Scientific

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Trypsin (0.25%), phenol red from Thermo Fisher Scientific

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