Trypsin Gold, Mass Spectrometry Grade V5280 from Promega

Product Specs
ItemTrypsin Gold, Mass Spectrometry Grade
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberV5280
FormatMass Spectrometry Grade
Molecule NameTrypsin Gold
Size100microg
Conjugate/TagUnconjugated

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) DMRT2 participates in chicken gonadal development by regulating CHUK and SOX9-mediated cell proliferation and apoptosisPoultry ScienceSeptember 1, 2026Xiao Lin, Zidi Jin, Aneeqa Imtiaz, Jing Ge, Haizhou Gong, Yihui Zhang, Yun Zheng, Minmeng Zhao, Daoqing Gong, Long Liu, et al.(Cat. No. I6125-25G, Sigma, MI, USA). Subsequently, triethylammonium bicarbonate, trypsin (Cat. No. V5280, Promega, Madison, USA), CaCl2, acetonitrile, and formic acid (Cat. No. A117-50, Thermo Fisher Scientific,

(2) E3 ubiquitin-ligase Hakai regulates LRP4 stability and Wnt/β-catenin signalling in colorectal cancer cellsiScienceAugust 21, 2026Andrea Rodríguez-Alonso, Lía Jove, Gloria Alfonsín, Marta Tasende, Ingrid Jordens, Madelon Maurice, Angélica Figueroa,Santa Cruz Biotechnology,Sc-2002 ProLong Gold,Invitrogen,P36930 Hoechst 33342,Invitrogen,H3570 Grade-modified Trypsin,Promega,V5280 ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, Pierce™ BCA Protein Assay Kit

(3) Loss of Rab8a-Dependent Tethering of Lipid Droplets to Mitochondria Contributes to Hypoxia-Reoxygenation InjuryMedComm (2020)August 6, 2026Jialing Tang, Nathan Joyce, Thomas Laval, Eddie Tam, Hye Kyoung Sung, Mireille Ouimet, Gary Sweeneymin on ice. Cell lysate was then subjected to in‐solution digestion following protocol by Promega (V5280). Gene ontology enrichment analysis was performed following the guidelines from the protocol using Cytoscape

(4) Proteome dynamics reveal Leiomodin 1 as a key regulator of myogenic differentiationElifeAugust 4, 2026Ellen Späth, Svenja C Schüler, Ivonne Heinze, Therese Dau, Alberto Minetti, Maleen Hofmann, Katja Hönzke, Julia von Maltzahn, Alessandro Ori26777,Bead acetylation for BioID "Peptide, recombinant Protein",Trypsin (sequencing grade),Promega,V5280,"Digestion (1:100, w/w)" "Peptide, recombinant protein",Lys-C,FUJIFILM Wako,Cat#: 121–05063,"Digestion

(5) Phosphatidylserine and RhoB connect PI4P and PA metabolism to maintain plasma membrane identityJournal of Cell BiologyAugust 3, 2026Shiying Huang, Yeun Ju Kim, Xiaofu Cao, Timothy W. Bumpus, Mira Sohn, Matthew Tyler Menold, Ryan K. Dale, Jin Joo Kang, Saori Uematsu, Shagun Gupta, et al.Polybrene Infection/Transfection Reagent, Sigma-Aldrich (TR-1003); Trypsin Gold Mass Spectrometry Grade, Promega (V5280); D-biotin, Chem-Impex (00033, Cas #: 58-85-5); (+)-S-Trityl-L-cysteine, Alfa Aesar (L14384, CAS #:

(6) Assessment of Allergenic Potential for Cross‐Reactivity of Olive ( Olea europaea ) Pit Xylo‐Oligosaccharide‐Rich Extract With Legislated Allergens in the European UnionFood Sci NutrAugust 3, 2026Maame Ekua Manful, Joseph O'Shea, Antía Torres, Paz Otero, Catherine Barry-Ryaniodoacetamide for 30 min. The digestion was carried out by incubating with 40 ng of trypsin (Promega V5280) at 37°C overnight, and tryptic peptides were extracted from the gel matrix in two steps with 0.5% trifluoroacetic

(7) Extracellular vesicles from cytokine-treated human pancreatic ductal cells enhance HLA class I expression in beta cellsFront ImmunolJuly 23, 2026Neslihan Erdem, Heather N. Zook, Janine C. Quijano, Nathaniel P. Hansen, Joanna Palade, Eunjin Oh, Jacob Mares, Cecile Donohue, Jose A. Ortiz, Kevin Jou, et al.temperature. Samples were diluted 2-fold and digested overnight with trypsin (Trypsin Gold, Promega, V5280) at an enzyme to substrate ratio of 1:50. Digests were quenched with trifluoric acid and then desalted

(8) Multi-omics profiling unveils biological and clinical insights into pulmonary sarcomatoid carcinomaCell Reports MedicineJuly 21, 2026Liangdong Sun, Jue Wang, Yilv Yan, Shengnan Duan, Di Wang, Jiameng Hu, Huansha Yu, Ningrui Wang, Min Zhang, Run Zhang, et al.Then three volumes of 20mM Tris-Cl, pH 7.5 was used to dilute the 8 M Urea to 2M. Trypsin (Promega, #V5280) was then added at a 1:100 enzyme-to-protein ratio for an overnight digestion at 37°C in a 2 M urea

(9) A Practical Experimental Protocol for Identification and Validation of UFMylation Substrate in Human CellsBio ProtocJuly 20, 2026Qian Liang, Yaoyao Fang, Juexi Dong, Xingling Yi, Yu-Sheng Cong(Sigma-Aldrich, CAS number: 10035-04-8) 22. Trypsin Gold, mass spectrometry grade (Promega, catalog number: V5280) 23. Trifluoroacetic acid (TFA) (Sigma-Aldrich, CAS number: 76-05-1) 24. Tween-20 (BioFroxx, catalog

(10) SIRT3 deficiency drives alveolar epithelial mitochondrial dysfunction in bronchopulmonary dysplasia via PDHA1 hyperacetylationiScienceJuly 17, 2026Cong Zhao, Hua Peng, Yalan Liu, Yan Chen, Chao Tong, Qianxue Zheng, Ziang Liu, Chunyan Chen, Jiayun Zhang, Wenbo Zhu, et al.buffer,Abbkine,Cat#KTD105-CN Protein A + G Magnetic Beads,Abbkine,Cat#KTD105-CN Sequencing-grade trypsin,Promega,Cat#V5280 ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, Mycolor One-Step

(11) 3.75 THz Repetitive Radiation Regulates Collagen Metabolism in Human Fetal Scleral FibroblastsCellsJuly 17, 2026Wenxia Wang, Liu Sun, Lei Wang, Jinwu Zhao, Pandeng Hou, Mingxia Hein order to quantify the total protein concentration. After trypsin-gold (Promega, Madison, WI, USA, V5280) was used to digest these protein samples, they were slowly put onto a C18 deionization column to desalt

(12) From monthly volatile dynamics to cultivar differences in the aroma of jasmine (Jasminum sambac): integrated volatile profiling and proteomic insightsFood Chem XJuly 16, 2026Yuhang Zhang, Weiwei Ren, Wenjing Gui, Shanshan Zeng, Pengjie Wang, Shan Jin, Kunbo Wang, Mengya Gu, Qin Luo, Naixing YeThen the solution was immediately placed in a dark room for iodoacetamide alkylation. 2 μL of trypsin (V5280, Promega, Wisconsin, USA) was added to 800 μL of ammonium bicarbonate solution (25 mmol/L), and the

(13) Deep Proteomic Analysis With Machine Learning Identifies Aqueous Humor Biomarkers of ADAMTSL4-associated Congenital Ectopia LentisInvest Ophthalmol Vis SciJuly 9, 2026Xinyao Chen, Xin Shen, Wannan Jia, Yalei Wang, Qiuyi Huo, Yanbo Xiao, Yulin Zhang, Linzhao Li, Xuqing Gao, Guangqi A, et al.Bradford assay kit (Beyotime, Shanghai, China). Proteins were digested with sequencing-grade trypsin (V5280; Promega, Madison, WI, USA) in 100-mM TEAB at 37°C for 4 hours. Formic acid (Thermo Fisher Scientific)

(14) Tumor Immune Cell Targeting Chimeras (TICTACs) reprogram tumor-associated macrophagesNat Chem BiolJuly 8, 2026Mariko Morimoto, David S. Roberts, Ru M. Wen, Ell Handy, Eric E. Peterson, Grace M. Stepek, Nicholas A. Till, James D. Brooks, Carolyn R. Bertozzithen digested on the S-trap using 50 μL of a 1:20 enzyme to substrate mass ratio of Trypsin (Promega, V5280) at 37 °C overnight for 16 h. The resulting peptides were then eluted from the S-trap using 40 μL of

(15) Structure and receptor recognition of type-II feline infectious peritonitis virus spike glycoproteinEMBO JJuly 8, 2026Sheng Niu, Yuyang Tian, Linh Nguyen, Chongzhi Bai, Xiaohan Hou, Dan Wang, Ziqian Niu, Zhimin Liu, Jianle Ren, Wentao Li, et al.Solution,Coolaber,Cat#SL4140 Glycine,Coolaber,Cat#CG5831 Urea,Sigma-Aldrich,Cat#U5128 Iodoacetamide,Sigma-Aldrich,Cat#I6125 DL-Dithiothreitol,Sigma-Aldrich,Cat#D0632 "Trypsin Gold, Mass Spectrometry Grade",Promega,Cat#V5280 "Lys-C, Sequencing Grade",Roche,Cat#11420429001 "Chymotrypsin, Sequencing Grade",Promega,Cat#V1061 Elastase,Promega,Cat#V1891 Glu-C (Sequencing Grade

(16) msBayesImpute as a versatile framework for addressing missing values in biomedical mass spectrometry proteomics dataCommun ChemJuly 7, 2026Jiaojiao He, Barbara Helm, Franziska Gödtel, Katharina Büchner, Marcel Schilling, Marc A. Schneider, Laura V. Klotz, Jana Braunger, Hauke Winter, Britta Velten, et al.cleanup procedure. For protein digestion buffer a solution containing 2.2 μg Trypsin Gold (PROMEGA # V5280)/75 μL 100 mM TEAB was prepared (1:25 protease to protein ratio). After preparation of the deck overlay

(17) EphB1-Mediated Transient Blood-Brain Barrier Opening Facilitates a Ferritin-Based Nanotherapeutic for Alzheimer's DiseaseAdv Sci (Weinh)July 6, 2026Shilin Wen, Jingjing Gao, Zhixian Wang, Qiumin Ma, Xiao-Ling Xu, Jianer Chen11 mM for 15 min. Subsequently, 800 µL of 25 mM ammonium bicarbonate and 2 µL of trypsin (Promega, V5280) were added, and the mixture was digested overnight at 37°C. The resulting peptides were acidified to

(18) A Proteomic Study of Differences in Muscle Quality Between the Longissimus Dorsi and Biceps Femoris Muscles in Junggar Bactrian CamelsBiology (Basel)July 6, 2026Yongbin Cai, Jintao Gan, Lirong Song, Zhixin Lu, Ye Qin, Wanlu Ren, Jianwen Wang, Xinkui Yao, Jun Meng, Yaqi ZengBradford Protein Quantification Kit (Beyotime, Shanghai, China), Mass Spectrometry-Grade Trypsin (Promega/V5280, Promega (Beijing) Biotech Co., Ltd., Beijing, China), Triethylammonium Bicarbonate Buffer (TEAB, Sigma/T7408-500ML,

(19) Hexosamine biosynthesis drives hemocyanin O-GlcNAcylation to potentiate antibacterial immunity in shrimpJournal of Biological ChemistryJuly 1, 2026Qian Feng, Maoshuai Fu, Wei Wang, Tingchu Wu, Junjie Nie, Zhihong Zheng, Yongzhen Zhao, Jude Juventus Aweya, Yueling Zhangpreviously described (15). Briefly, gel pieces were destained, digested with Trypsin Gold (Promega, V5280), and the resulting peptides were analyzed using an Easy nLC 1200 system coupled to a Q Exactive mass

(20) Rapid Histone Post-Translational Modification Analysis Using Alternative Proteases and Tandem Mass TagsAnalytical ChemistryJune 30, 2026Natalie P. Turner, Sabyasachi Baboo, Patrick Garrett, Jolene K. Diedrich, Michal Bajo, Marisa Roberto, John R. Yates IIIsamples were resuspended in 10 μL digestion buffer containing Trypsin (Trypsin Gold, MS Grade, Promega, V5280) in 100 mM AMBIC pH 8.5. For the ‘Trypsin + Urea’ group, the digestion buffer also contained 2 M Urea

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Trypsin Gold, Mass Spectrometry Grade

Trypsin Gold, Mass Spectrometry Grade from Promega

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Trypsin Gold, Mass Spectrometry Grade from Promega

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