cAMP-Dependent Protein Kinase, Catalytic Subunit V5161 from Promega

Product Specs
ItemcAMP-Dependent Protein Kinase, Catalytic Subunit
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberV5161
SpeciesHuman
Conjugate/TagUnconjugated
Format>90% Pure
Size2,500u
Source/Expression SystemBovine Heart
Molecule NamecAMP-Dependent Protein Kinase

Add to Compare List

Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Structural determinants of protein kinase A essential for CFTR channel activationProceedings of the National Academy of SciencesNovember 12, 2024Csaba Mihályi, Iordan Iordanov, Andras Szollosi, László Csanády539576 (SI Appendix, Fig. S3 A and C)] functionally resembled our bPKA-C, recombinant products (Promega V5161) recapitulated our findings on rPKA-C (SI Appendix, Fig. S3 B and C). These results suggest that the

(2) Activity- and sleep-dependent regulation of tonic inhibition by Shisa7Cell reportsMarch 23, 2021Wu K, Han W, Tian Q, Li Y, Lu W.G,Thermo Fisher Scientific,Cat# 10003D "cAMP-Dependent Protein Kinase, Catalytic Subunit",Promega,Cat# V5161 Protein Kinase C,Promega,Cat# V5261 Experimental models: cell lines,Experimental models: cell lines,Experimental models

(3) FXYD protein isoforms differentially modulate human Na/K pump functionJournal of General PhysiologyDecember 7, 2020Dylan J. Meyer, Sharan Bijlani, Marilina de Sautu, Kerri Spontarelli, Victoria C. Young, Craig Gatto, Pablo Artigasoocytes were Na+-loaded for 30 min (as above), kept in Na+ solution for 30 min, injected with PKA (∼2 µM, V5161; Promega) or PKI (∼250 µM), and kept in Na+ solution for ≥45 min until recording. Oocyte volume was

(4) S92 phosphorylation induces structural changes in the N-terminus domain of human mitochondrial calcium uniporterScientific ReportsDecember 1, 2020Youngjin Lee, Jongseo Park, Gihwan Lee, Sanghwa Yoon, Choon Kee Min, Tae Gyun Kim, Takenori Yamamoto, Do Han Kim, Keun Woo Lee, Soo Hyun EomNTDAAS92, and 32 μg of commercially obtained MBP (Enzo, ALX-202-075) were phosphorylated by PKA (Promega, V5161), PKC mixtures (α, β, and γ isoforms with lesser δ and ζ; Promega, V5261), PKCβII (Promega, V3741),

(5) Chemical Genetics of AGC-kinases Reveals Shared Targets of Ypk1, Protein Kinase A and Sch9*Molecular & Cellular ProteomicsApril 1, 2020Michael Plank et al.Bradford assay) was mixed with 0.5 μm GST-Ypk1 purified from S. cerevisiae or bovine PKA (Promega; V5161) and 300 μm ATP in 25 mm HEPES pH 7.5, 150 mm KCl, 2.5 mm CHAPS, 5 mm MgCl2, 1 mm DTT. After 10 min

(6) Increased myocyte calcium sensitivity in end-stage pediatric dilated cardiomyopathyAmerican Journal of Physiology-Heart and Circulatory PhysiologyDecember 1, 2019Stephanie J. Nakano et al.permeabilized myocyte slurry by addition of 10 ␮L of a 1 U/mL solution of the PKA catalytic subunit (V5161; Promega Inc.) in 350 mM KH2PO4 buffer. Ten microliters of 350 mM KH2PO4 buffer was added to the non-PKA-treated

(7) The BCKDH Kinase and Phosphatase Integrate BCAA and Lipid Metabolism via Regulation of ATP-Citrate LyaseCell metabolismJune 5, 2018White PJ, McGarrah RW, Grimsrud PA, Tso SC, Yang WH, Haldeman JM, Grenier-Larouche T, An J, Lapworth AL, Astapova I, et al.Chem-Impex Int. INC Cat# 25643 GST-tagged human ACL,Sigma,SRP0288 40 KDa subunit of bovine PKA,Promega,V5161 Critical Commercial Assays,Critical Commercial Assays,Critical Commercial Assays Rat Insulin ELISA,EMD Millipore

(8) Menin and PRMT5 suppress GLP1 receptor transcript and PKA-mediated phosphorylation of FOXO1 and CREBAmerican journal of physiology. Endocrinology and metabolismAugust 1, 2017Muhammad AB, Xing B, Liu C, Naji A, Ma X, Simmons RA, Hua X.performed using PKA assay kit (cat. no. 17–134; Millipore) and recombinant PKA catalytic subunit (cat. no. V5161; Promega, Madison, WI), according to the manufacturer’s instructions with slight modifications. Briefly,

(9) Fasting and Systemic Insulin Signaling Regulate Phosphorylation of Brain Proteins That Modulate Cell Morphology and Link to Neurological DisordersThe Journal of biological chemistryDecember 11, 2015Li M, Quan C, Toth R, Campbell DG, MacKintosh C, Wang HY, Chen S.purified using glutathione-Sepharose 4B (GE Healthcare), and phosphorylated by a catalytic subunit of PKA (V5161, Promega) in vitro at 30 °C for 30 min as described previously (20). GAP Activity Measurement The

(10) PKA and CDK5 can phosphorylate specific serines on the intracellular domain of podoplanin (PDPN) to inhibit cell motilityExperimental cell researchJuly 1, 2015Krishnan H, Retzbach EP, Ramirez MI, Liu T, Li H, Miller WT, Goldberg GS.0.25 mM ATP, and [γ-32P]-ATP (100 cpm/pmol). Reactions were initiated by adding 80 µg/ml PKA (Promega V5161, Madison, WI, USA), 22 µg/ml CDK5/p25 kinase enzyme (Promega V3231, Madison, WI, USA), or both PKA and CDK5/p25.

(11) Multiplex Enzyme Assays and Inhibitor Screening by Mass SpectrometryJ Biomol ScreenSeptember 1, 2010Rakesh Rathore, Patrick Pribil, Jay J. Corr, William L. Seibel, Artem Evdokimov, Kenneth D. Greispurchased from Sigma-Aldrich (St. Louis, MO). The catalytic subunit of cAMP-dependent protein kinase (PKA, V5161) and Kemptide (V5601) were purchased from Promega (Madison, WI). Ammonium monobasic phosphate and formic

(12) Development of an Inhibitor Screening Platform via Mass SpectrometryJ Biomol ScreenDecember 1, 2008Rakesh Rathore, Jay Corr, George Scott, Pauline Vollmerhaus, Kenneth D. Greisdiscussed. MATERIALS AND METHODS Materials The catalytic subunit of cAMP-dependent protein kinase (PKA, V5161) and kemptide (V5601) were purchased from Promega (Madison, WIi). α-Cyano-4-hydroxycinnamic acid (4-HCCA),

(13) MALDI-TOF MS as a label-free approach to rapid inhibitor screeningJournal of the American Society for Mass SpectrometryJune 1, 2006Kenneth D. Greis, Songtao Zhou, Thomas M. Burt, Andrew N. Carr, Elizabeth Dolan, Vijayasurian Easwaran, Artem Evdokimov, Richard Kawamoto, Jeff Roesgen, Gregory F. Davis

(14) A simple method using 31 P -NMR spectroscopy for the study of protein phosphorylationBrain Research ProtocolsJanuary 27, 2000Hirokazu Hirai, Keitaro Yoshioka, Kazuhiro Yamada

Be the First to Write a Review!

cAMP-Dependent Protein Kinase, Catalytic Subunit

cAMP-Dependent Protein Kinase, Catalytic Subunit from Promega

Description

Promega

Citations (14)

Reviews

cAMP-Dependent Protein Kinase, Catalytic Subunit from Promega

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map