Published customer image: Recombinant Human FGF Basic (PHP105) used to supplement tissue cultuure medium supporting growth of human and canine chondrocyte like cells (CLCs) in vitro.Image caption:Indian and Sonic hedgehog facilitate calcification in human and canine chondrocyte‐like cells in vitro. (A) Hedgehog and PTHrP signaling‐related mRNA expression in canine CLC monolayers treated with hypertrophic induction medium supplemented with/without 1 μg/mL IHH or SHH for 7 days. n = 4. **P <.01. ALP, alkaline phosphatase; IHH, Indian hedgehog; PTHR1, PTHrP receptor 1, RUNX2, Runt‐related transcription factor 2; SHH, Sonic hedgehog. Alizarin Red S staining on one representative canine (B) and human (C) CLC donor. CLC monolayers were treated with hypertrophic induction medium supplemented with/without 1 μg/mL IHH or SHH for 7 days. n = 4 per speciesFrom: Bach FC, de Rooij KM, Riemers FM, Snuggs JW, de Jong WAM, Zhang Y, Creemers LB, Chan D, Le Maitre C, Tryfonidou MA.Hedgehog proteins and parathyroid hormone-related protein are involved in intervertebral disc maturation, degeneration, and calcification.JOR Spine. 2019 Nov 19;2(4):e1071.doi: 10.1002/jsp2.1071.This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Published customer image: Recombinant Human FGF Basic (PHP105) used to supplement tissue cultuure medium supporting growth of human and canine chondrocyte like cells (CLCs) in vitro.Image caption:Indian and Sonic hedgehog facilitate calcification in human and canine chondrocyte‐like cells in vitro. (A) Hedgehog and PTHrP signaling‐related mRNA expression in canine CLC monolayers treated with hypertrophic induction medium supplemented with/without 1 μg/mL IHH or SHH for 7 days. n = 4. **P <.01. ALP, alkaline phosphatase; IHH, Indian hedgehog; PTHR1, PTHrP receptor 1, RUNX2, Runt‐related transcription factor 2; SHH, Sonic hedgehog. Alizarin Red S staining on one representative canine (B) and human (C) CLC donor. CLC monolayers were treated with hypertrophic induction medium supplemented with/without 1 μg/mL IHH or SHH for 7 days. n = 4 per speciesFrom: Bach FC, de Rooij KM, Riemers FM, Snuggs JW, de Jong WAM, Zhang Y, Creemers LB, Chan D, Le Maitre C, Tryfonidou MA.Hedgehog proteins and parathyroid hormone-related protein are involved in intervertebral disc maturation, degeneration, and calcification..doi: 10.1002/jsp2.1071.This image is from an open access article distributed under terms of a.
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