Sequencing Grade Modified Trypsin, Frozen V5113 from Promega

Product Specs
ItemSequencing Grade Modified Trypsin, Frozen
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberV5113
Conjugate/TagUnconjugated
FormatSequencing Grade Modified, Frozen
Size5 x 20microg
Molecule NameTrypsin

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) A covalent allosteric molecular glue suppresses NRF2-dependent cancer growthCancer DiscoveryMay 1, 2026Nilotpal Roy, Tine Wyseure, I-Chung Lo, Justine Lu, Christie L. Eissler, Steffen M. Bernard, Ilah Bok, Aaron N. Snead, Albert Parker, U-Ging Lo, et al.(ABC). Beads were then resuspended in 100 μL of 50 mmol/L ABC containing 1 μg trypsin (Promega, cat. #V5113) and 0.1 mAu Lys-C (Wako Chemicals, cat. #129-02541) and incubated overnight at 37°C with shaking for

(2) Accumulated mtDNA mutations are linked to specific impairments in NADH-linked respirationiScienceApril 17, 2026Edziu Franczak, McLane M. Montgomery, Zoe S. Terwillinger, Raphael T. Aruleba, Polina Krassovskaia, Ilya N. Boykov, James T. Hagen, Emely A. Pacheco, Brett R. Chrest, Tonya N. Zeczycki, et al.A117 Acetonitrile,Thermo-Fisher,Cat#: A955 Endoproteinase LysC,Thermo-Fisher,Cat#: 90307 Seq Grade Modified Trypsin,Promega,Cat#: V5113 Iodoacetamide,Sigma-Aldrich,Cat#: I1149 Pierce Trifluoroacetic Acid,Thermo-Fisher,Cat#: 28904 DL-Dithiothreitol,Sigma-Aldrich,Cat#:

(3) Nutritional supplementation remodels yak cardiac molecular networks through selenoprotein activation and immune quiescenceFront NutrApril 14, 2026Yiqun Chen, Chaoyun Yang, Tao Li, Guojun Chen, Yicheng Wang, Zhiqiang Hu, Jun Li, Jing Wang, Ran Guan, Zengwen HuangProteins were digested with sequencing-grade modified trypsin (Promega, Madison, WI, USA; Cat. No. V5113) at an enzyme-to-protein ratio of 1:50 (w/w) at 37 °C for 16 h. Peptides were eluted by centrifugation,

(4) A bacterial family of fatty acid acyltransferases related to the Shigella effector IcsBmBioApril 8, 2026Waad Bajunaid, Kyle Tomaro, Anwer Hasil Kottarampatel, Geneviève F. Desrochers, Mathieu Lavallée-Adam, John P. Pezacki, François-Xavier Campbell-Valois(Thermo Scientific, 90114) buffer and stored in 100 µL TEAB buffer at 4°C until adding trypsin (Promega, V5113); 2 μL of 0.5 mg/mL trypsin was added and rotated at 37°C overnight. Beads were pelleted the next day,

(5) DNA O-MAP uncovers the molecular neighborhoods associated with specific genomic locieLifeApril 7, 2026Yuzhen Liu, Christopher D McGann, Conor P Herlihy, Mary Krebs, Thomas A Perkins, Rose Fields, Conor K Camplisson, David Z Nwizugbo, Qiaoyi Lin, Nicolas J Longhi, et al.in a 50-µl volume and incubated for 16 hr with vortexing. The next day, 200 ng of trypsin (Promega V5113) was added to the beads and incubated for 6 hr at 37°C at 200 rpm. For solid phase samples, 20 ng of

(6) The COX2-PGE2-PKA Axis Suppresses Antiviral Immunity by Inhibiting mtDNA-Dependent STING ActivationbioRxivApril 6, 2026Pham Thuy Tien Vo, Julien Cicero, Zichen Wang, Hiroyuki Hakozaki, Thomas S Hoang, Sendi Rafael Adame-Garcia, Dana J. Ramms, Kuniaki Sato, Erica Stevenson, Yuan Zhou, et al.incubated for 45 min at room temperature in the dark. Next, 3 mM dithiothreitol and 1 μg of trypsin (V5113, Promega) were added to the samples, which were incubated at 37°C overnight. The next day, samples were

(7) G protein Gαq subunits engage targets in the nucleus involved in chromatin remodeling and gene expressionJournal of Biological ChemistryApril 1, 2026Joseph Loomis, Naincy Chandan, Michael Burroughs, Saji Abraham, Rongxi Zhang, Yiyi Cao, Matthew J. Brody, Gregory G. Tall, Alan V. Smrckaat room temperature (RT) for 30 min in the dark. Third, samples were digested with trypsin (Promega, V5113) at 37 °C. For this, a 1:25 trypsin:protein ratio was used, and samples were subjected to constant mixing

(8) Pan-neurodegeneration proteomics reveals disease subtypes and molecular signaturesCellMarch 23, 2026Him K. Shrestha, Huan Sun, Jay M. Yarbro, DongGeun Lee, Danting Liu, Erming Wang, Meghan McReynolds, Nan Zhang, Boer Xie, Shu Yang, et al.size)",Waters,Cat# 186002354 Lys-C,Wako,Cat# 12505061 Sequencing-grade modified trypsin,Promega,Cat# V5113 Glass beads,ThermoFisher Scientific,Cat# NC0103402 Acetonitrile,Honeywell,Cat# AH-015-4 BCA protein assay kit

(9) Mag-Net Strong Anion Exchange Enables Isolation of Ovarian Cancer Ascites Extracellular Vesicles for Proteomic Biomarker DiscoveryBio ProtocMarch 20, 2026Tyler T. Cooperbicarbonate (Sigma-Aldrich, catalog number: 1066-33-7) 17. Sequencing-grade trypsin (Promega, catalog number: V5113) 18. Sequencing-grade LysC (FujiFilm Bioscience, catalog number: 125-05061) 19. LC–MS-grade 0.1% in

(10) Mag-Net Strong Anion Exchange Enables Isolation of Ovarian Cancer Ascites Extracellular Vesicles for Proteomic Biomarker DiscoveryBIO-PROTOCOLMarch 20, 2026Tyler T. Cooperbicarbonate (Sigma-Aldrich, catalog number: 1066-33-7) 17. Sequencing-grade trypsin (Promega, catalog number: V5113) 18. Sequencing-grade LysC (FujiFilm Bioscience, catalog number: 125-05061) 19. LC–MS-grade 0.1% in

(11) LENG8 mediates RNA nuclear retention and degradation in eukaryotesMol CellMarch 19, 2026Lusong Tian, Liang Liu, Yoseop Yoon, Lindsey V. Soles, Marielle Valdez, Joshua Jeong, Clinton Yu, Lan Huang, Yongsheng Shiurea concentration was diluted to 1–1.5 M by adding 25 mM ammonium bicarbonate, and trypsin (Promega, V5113) was added at 2% of the total protein content (e.g., 2 μg for 100 μg protein), also reconstituted in

(12) Multi-omic profiling converges on proteasome subunits PSMA7/PSMB2 as targets of the sepsis-protective agent HandelinFrontiers in ImmunologyMarch 19, 2026Dexiu Chen, Qian Zhang, Yuanxin Wu, Yingchun Hu, Muhu Chenbicarbonate (TEAB, Sigma, T7408) and digested overnight at 37°C with sequencing-grade trypsin (Promega, V5113) at an enzyme-to-substrate ratio of 1:50 (w/w). The resulting peptides were desalted using C18 StageTips,

(13) Role of small intronic RNAs in the crosstalk between immune cells and β-cells during type 1 diabetes developmentRNA BiolMarch 13, 2026Shagun Poddar, Flora Brozzi, Cristina Cosentino, Cécile Jacovetti, Claudiane Guay, Jérôme Perrard, Romano Regazziethanol, beads were digested in 50 ul of 100 mM ammonium bicarbonate with 1.0 ug of trypsin (Promega #V5113). After 1 h of incubation at 37°C, the same amount of trypsin was added to the samples for an additional

(14) Exon inclusion signatures enable accurate estimation of splicing factor activityNat CommunMarch 12, 2026Miquel Anglada-Girotto, Carolina Segura-Morales, Daniel F. Moakley, Chaolin Zhang, Samuel Miravet-Verde, Andrea Califano, Luis Serrano2-fold with 200 mM ammonium bicarbonate for trypsin digestion (1:10 w:w, 37 °C, over 8 h, Promega cat # V5113). After digestion, peptide mix was acidified with formic acid and desalted with a MicroSpin C18 column

(15) The yeast phosphofructokinase β-subunit has RNA unwinding activity and modulates cell cycle progressionNucleic Acids ResMarch 12, 2026Waleed S Albihlal, Ana M Matia-González, Tobias Schmidt, Wieland Mayer, Joe Bryant, Martin Bushell, Dierk Niessing, Matteo Barberis, Alexander Schmidt, Jürgen J Heinisch, et al.S5. Enzymes: AcTEV™ protease (Invitrogen, 12575-023), Proteinase K (Invitrogen, AM2546), Trypsin (Promega, V5113), T7 RNA polymerase (NEB, M0251), RNase A (Thermo Fisher, EN0531), RNasin (Promega, N2511), SUPERase•In™

(16) Characterization of Borrelia-Derived Extracellular Vesicles: Implications for Pathogenesis and DiagnosticsMicroorganismsMarch 7, 2026Barbara Birkaya, Ahana Byne, Sumaiya Irfan, Joseph Gallagher, Dominic Granato, Hayat Kharmoud, Andrea Blake Brothers, Elsa Ronzier, Amanda Haymond Still, Weidong Zhou, et al.digestion ran overnight with 2 µL of (0.5 µg/µL) of sequencing-grade trypsin (Promega, Madison, WI, USA, V5113) in 50 mM ammonium bicarbonate, pH 8, at 37 °C. A total of 2 µL of 100% trifluoroacetic acid (TFA) was

(17) The Disordered Region of ASXL1 Acts as an Auto‐Regulator Through CondensationAdvanced ScienceMarch 1, 2026Xiao Fang, Qiwei Li, Wenqing ZhangABC buffer (50 mm ammonium bicarbonate, pH 8). On‐bead digestion was performed with trypsin (Promega, V5113) for 18 h, as previously described [63]. The resulting peptides were then dried and submitted for mass spectrometry (MS

(18) Protocol for stable isotopic tracing to assess cellular lipogenic activity in induced neural stem cellsSTAR ProtocolsMarch 1, 2026Rosana-Bristena Ionescu, Julie A. Reisz, Monika Dzieciatkowska, Daniel Stephenson, Alexandra M. Nicaise, Pranathi Prasad, Cory M. Willis, Marta Suarez Cubero, Luca Peruzzotti-Jametti, Frank Edenhofer, et al.bicarbonate,Sigma-Aldrich,Cat#09830 Urea,Sigma-Aldrich,Cat# U5378 Phosphoric acid,Sigma-Aldrich,Cat#452289 Sequencing grade trypsin,Promega,Cat#V5113 Isopropanol,Fisher Scientific,Cat# A461 Calibration solutions,Fisher Scientific,Cat#PI88323 and PI88324 ,, Software

(19) Ketogenic diet dampens excitatory neurotransmission by shrinking synaptic vesicle poolsCell ReportsFebruary 24, 2026Marion I. Stunault, Pan-Yue Deng, Anjali Yadav, Erica M. Periandri, Francisca N. de Luna Vitorino, Michael B. Thomsen, Jasmin Sponagel, Amelia J. Barfield, Renzelle J. Ponce, Layla Foroughi, et al.50 mM ammonium bicarbonate and incubated with sequencing-grade modified trypsin (Promega, Cat. No. V5113) in a 1:20 enzyme:sample ratio overnight at room temperature. Following trypsin digestion, derivatization

(20) Cooperative Architecture of Mitochondrial Proteome HomeostasismedRxivFebruary 11, 2026Patrick Forny, Merima Forny, Andrew J. Smith, Andrew Y. Sung, Kaixian Liu, David J. Pagliarinitemperature (1000 rpm) for reduction and alkylation. Proteins were digested with trypsin (Promega, V5113 or Thermo, 90305) at a 1:50 enzyme-to-protein ratio, ensuring a final urea concentration below 2 M.

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4/12/2019Modified Trypsin for Proteomics Sample Preparation Zan ChenIt's a reliable trypsin for proteomics experiments.

Sequencing Grade Modified Trypsin, Frozen

Sequencing Grade Modified Trypsin, Frozen from Promega

Description

Promega

Citations (608)

Reviews

Protocols

Extraction of Proteins from Formalin- Fixed, Paraffin- Embedded Tissue Using the Qproteome Extraction Technique and Preparation of Tryptic Peptides for Liquid Chromatography/Mass Spectrometry Analysis

Isolation of Proteins by Heat- Induced Extraction from Formalin- Fixed, Paraffin- Embedded Tissue and Preparation of Tryptic Peptides for Mass Spectrometric Analysis

Sequencing Grade Modified Trypsin, Frozen from Promega

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