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DNA-Dependent Protein Kinase
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DNA-Dependent Protein Kinase
DNA-Dependent Protein Kinase
DNA-Dependent Protein Kinase from Promega
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Citations:
(5)
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DNA-Dependent Protein Kinase
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Description
DNA-Dependent Protein Kinase (DNA-PK) phosphorylates several DNA-binding substrates in vitro, including the tumor suppressor protein p53, the SV40 large T antigen and several transcription factors
Product Specs
Company Info
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Product Specs
Item
DNA-Dependent Protein Kinase
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
V5811
Species
Human
Conjugate/Tag
Unconjugated
Size
2,500u
Molecule Name
DNA-Dependent Protein Kinase
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Promega
2800 Woods Hollow Rd
Madison
,
WI
53711
United States
Phone
: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516
Email: custserv@promega.com
techserv@promega.com
Company Profile
Website:
http://www.promega.com
Citations (5)
(1)
PARylation of GCN5 by PARP1 mediates its recruitment to DSBs and facilitates both HR and NHEJ Repair
Cellular and Molecular Life Sciences
December 1, 2024
Debashmita Sarkar, Amartya Chakraborty, Shaina Mandi, Shilpee Dutt
GCN5 (Novus Biologicals, NBP1-72,426) and 0.5, 1, 1.5, 2, 2.5 units of purified DNA-PK enzyme (
Promega
,
V5811
) or 0.1 µM DNA-PK wild type, K3241A, K3260A, K3241A + K3260A mutant peptides (Supplementary Table S5)
(2)
Flexible Tethering of ASPP Proteins Facilitates PP-1c Catalysis
Structure
October 1, 2019
Yeyun Zhou, Robyn Millott, Hyeong Jin Kim, Shiyun Peng, Ross A. Edwards, Tamara Skene-Arnold, Michal Hammel, Susan P. Lees-Miller, John A. Tainer, Charles F. B. Holmes,
et al.
site-directed mutagenesis
kit,Stratagene,Cat# 200521
DNA-Dependent Protein Kinase
(DNA-PK),
Promega
,Cat#
V5811
Reversible Protein Stain Kit,Thermo Scientific/
Pierce
,Cat#
24585
Deposited Data,Deposited Data,Deposited
(3)
Intrinsically disordered sequences enable modulation of protein phase separation through distributed tyrosine motifs
The Journal of biological chemistry
November 17, 2017
Lin Y, Currie SL, Rosen MK.
with 12.5 μm PRM4 (molecule not module concentration) with or without 114.1 units of DNA-PK (
Promega
,
V5811
) at room temperature (22 °C). One unit is defined as the amount of enzyme required to incorporate 1
(4)
ATM regulates proteasome-dependent subnuclear localization of TRF1, which is important for telomere maintenance
Nucleic acids research
May 1, 2012
McKerlie M, Lin S, Zhu XD.
wild-type TRF1 (2 µg) or TRF1-S367A (2 µg) was incubated with 20 units of purified DNA-PKcs (
Promega
,
V5811
) in the presence of γ-32P-ATP according to the manufacturer's instruction.
Immunofluorescence
and
fluorescence in situ hybridization
(5)
IKKβ Regulates the Repair of DNA Double-Strand Breaks Induced by Ionizing Radiation in MCF-7 Breast Cancer Cells
PLoS ONE
April 7, 2011
Lixian Wu, Lijian Shao, Ningfei An, Junru Wang, Senthil Pazhanisamy, Wei Feng, Martin Hauer-Jensen, Shigeki Miyamoto, Daohong Zhou
Madison, WI). The biotinylated peptide substrate was incubated with 50 units purified DNA-PK (Cat#
V5811
,
Promega
) and (γ-32P)ATP in the presence or absence of BMS (5 or 10 µM) or NU (5 µM) for 5 min at 30°C
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DNA-Dependent Protein Kinase from Promega
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