Proteinase K V3021 from Promega

Product Specs
ItemProteinase K
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberV3021
FormatPurified to remove Dnase and Rnase activities.
Conjugate/TagUnconjugated
Size100mg
Molecule NameProteinase K
Source/Expression SystemTritirachium album
TypeEnzyme

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Eemian palaeogenetics demonstrates loss of diversity in modern fallow deer (Dama dama)iScienceJune 19, 2026Alberto Rocha-Méndez, Patrick Arnold, Lutz Kindler, Sabine Gaudzinski-Windheuser, Wil Roebroeks, Fulco Scherjon, Michael Hofreiterv1.4.2,N/A,http://tree.bio.ed.ac.uk/software/figtree/ ,, Other,Other,Other ,, Proteinase K,Promega,Cat#V3021 FastAp,Thermo Fisher,Cat#EF0651 Dynabeads MyOne C1,Thermo Fisher,Cat#65001 T4 DNA Polymerase,Thermo Fisher

(2) Protocol for chromatin immunoprecipitation using isolated antheridia of Marchantia polymorphaSTAR ProtocolsMarch 20, 2026Keisuke Inoue, Kanta Kotani, Shohei Yamaoka, Takashi Araki"RNase A, DNase and protease-free (10 mg/mL)",Thermo Fisher Scientific,Cat#EN0531 Proteinase K (Lyophilized),Promega,Cat#V3021 SYBR Gold Nucleic Acid Gel Stain,Thermo Fisher Scientific,Cat#S11494 ,, Critical commercial assays,Critical

(3) Comparative embryogenesis of two salp species reveals rogue development and evolutionary divergence from sessile tunicatesPLoS BiolMarch 17, 2026Marie Lebel, Alexandre Alié, Patrick Lemaire, Stefano Tiozzobaskets in 24-well plates for further treatments. Samples were digested with a Proteinase K (Promega V3021) treatment at a concentration of 10 µg/ml for 30–45 min at 37 °C. The digestion was stopped with a quick

(4) In vivo genome-wide CRISPR screens in human T cells to enhance T cell therapy for solid tumorsbioRxivMarch 7, 2026Qi Liu, Peixin Amy Chen, Esha Urs, Shimin Zhang, Maya M. Arce, Charlotte H. Wang, Zhongmei Li, Jin Seo, Nupura Kale, Taylor N. LaFlam, et al.incubated at 37 °C for 2 hours, followed by the addition of 16 μL proteinase K (20 mg/mL, Promega, V3021) and incubation at 55 °C for 2 more hours. gDNA was then purified by extraction with phenol:chloroform:isoamyl

(5) Generalizable direct protein sequencing with InstaNexusMolecular & Cellular ProteomicsMarch 2, 2026Marco Reverenna, Maike Wennekers Nielsen, Darian Stephan Wolff, Jemma Daniel, Elpida Lytra, Suthimon Thumtecho, Pasquale D. Colaianni, Anne Ljungars, Andreas H. Laustsen, Erwin M. Schoof, et al.and 37 °C) for the following proteases: papain (Sigma-Aldrich, P5306-25MG), proteinase K (Promega, V3021), chymotrypsin (Promega, V1061), GluC (Promega, V1651), thermolysin (Promega, P1512), and elastase (Sigma-Aldrich,

(6) Cryo-EM structures of light chain fibrils from abdominal fat biopsies of multiple myeloma patientsNature CommunicationsFebruary 24, 2026Yuxuan Yao, Shun Yao, Yamei Xu, Qinyue Zhao, Xingyu Xiong, Kaien Liu, Yu Liu, Zhizhi Wang, Wenqing Xu, Cong Liu, et al.trypsin (Promega, V5111), chymotrypsin (Promega, V1061), Asp-N (Promega, V1621), proteinase K (Promega, V3021), a-lytic (New England Biolabs, P8113). Peptides were extracted twice with 0.1% trifluoroacetic acid

(7) Alphaviral Capsid Proteins Inhibit Stress Granule Assembly via Competitive RNA Binding With G3BP1Advanced ScienceJanuary 27, 2026Yun Zhang, Yi Liu, Zhiying Yao, Haolong Lai, Xiaoxin Chen, Ziqiu Wang, Yiqiong Bao, Tingting Li, Xiaoming Zhou, Xiabin Chen, et al.beads were resuspended in TBS containing RNase inhibitor, digested with 50 µg/mL Proteinase K (Promega, V3021) at 42°C for 15 min, The supernatant was collected and mixed with 3 volumes of TRIzol reagent to isolate

(8) Biopsy-resolved cryo-EM structures of amyloid fibrils provide molecular insights into AL amyloidosisProceedings of the National Academy of SciencesJanuary 13, 2026Yuxuan Yao, Qinyue Zhao, Shun Yao, Yamei Xu, Kaien Liu, Tianyi Cao, Bo Sun, Jingmin Zhou, Cong Liu, Dan Litrypsin (Promega, V5111), chymotrypsin (Promega, V1061), Asp-N (Promega, V1621), proteinase K (Promega, V3021), a-lytic (New England Biolabs, P8113) were added. For analysis of copurified protein, sequencing grade

(9) Ancient biomolecules suggest a learned foraging strategy in extinct cave bearsiScienceNovember 20, 2025Ioana N. Meleg, Federica Alberti, Dorothée G. Drucker, Magdalena Năpăruș-Aljančič, Angelica Feurdean, Marius Robu, Marius Vlaicu, Yuichi I. Naito, Adina Boroneanț, Marin Cârciumaru, et al.10.8.,N/A,https://desktop.arcgis.com/en/arcmap/latest/get-started/setup/arcgis-desktop-system-requirements.htm R version 4.3.1,61,http://www.R-project.org/ ,, Other,Other,Other ,, Proteinase K,Promega,Cat#V3021 Zymo-spin V column extension reservoir,Zymo,Cat#C1016-50 Circligase II,Biozym,Cat#131402(CL9021K) Endonuclease VIII

(10) Crude preparation of a phage-encoded biofilm-dispersing factor expressed in E. coli and its potential application in bacteriological analysis of environmental water samplesApplied and Environmental MicrobiologyNovember 19, 2025Shah Nayeem Faruque, Iftekhar Bin Naser, M. Mozammel Hoque, Fariya Akter, Shah M. Faruqueextract was mixed with 0.5 mL buffer (20 mM Tris-Cl pH 8.0, 50 mM EDTA, 1% SDS). Proteinase K (Cat no. V3021; 36 u/mg Promega) was added to a final concentration of 50 µg/mL, and the reaction was incubated at

(11) Identification of Unknown Biological Toxin Proteins Using Mass Spectrometry: A Case Study on De Novo Sequencing of RicinToxins (Basel)November 18, 2025Yubo Song, Hao Wang, Junjie Wen, Jiale Xu, Siyu Zhu, Fuli Wang, Yongqian Zhang(Promega, V511A), Glu-C (Promega, V1651), Chymotrypsin (Promega, V1061), and Proteinase K (Promega, V3021). The sample was first loaded into a 10 kDa molecular weight cut-off ultrafiltration centrifuge tube

(12) Suppressing protein damage response to overcome multidrug resistance in cancer therapyCell discoverySeptember 30, 2025Shao F, Li Z, Xiao H, Chen Y, Zhang Y, Li L, Peng Y, Li X, Hou Y, Li B, et al.cells were lysed. Half of the sample underwent limited proteolysis (LiP) with proteinase K (Promega, V3021) for 1 min at 25 °C. The proteinase K was then inactivated by boiling the sample in water. This was

(13) A Maternal and Postnatal Ad Libitum Propionic Acid-Rich Diet in Mice Alters Intestinal Glia Proliferation and Inflammatory Response: Contrary to Effect in the BrainInternational Journal of Molecular SciencesSeptember 23, 2025Piotr P. Lagod, Latifa S. Abdelli, Saleh A. Naserpublished protocol [56]. First, the tail snips were processed with lysis buffer (0.4 mg/mL proteinase K [V3021, Promega, Madison, WI, USA], 10 mM Tris, pH 8.00, 100 mM NaCl, 10 mM Ethylenediaminetetraacetic acid

(14) Liebenberg syndrome severity arises from variations in Pitx1 locus topology and proportion of ectopically transcribing cellsNat CommunJuly 9, 2025Olimpia Bompadre, Raquel Rouco, Fabrice Darbellay, Antonella Rauseo, Fanny Guerard-Millet, Claudia Gentile, Marie Kmita, Guillaume AndreyChromatin was eluted and de-crosslinked overnight with the addition of 5 μL Proteinase K (10 mg/mL, Promega V3021). RNase A (4 μL, 10 mg/mL, ThermoFisher EN0531) treatment followed, and then phenol:chloroform:IAA extraction

(15) A single cell atlas of the mouse seminal vesicleG3: Genes, Genomes, GeneticsMay 8, 2025Fengyun Sun, Kathleen Desevin, Yu Fu, Shanmathi Parameswaran, Jemma Mayall, Vera Rinaldi, Nils Krietenstein, Artür Manukyan, Qiangzong Yin, Carolina Galan, et al.(3 × 5 min) and a second antigen retrieval step at 37°C using proteinase K (20 μg/ml in TBS, pH 8, V3021, Promega), the slides were blocked as described above. This was followed by a second primary antibody

(16) Spt5 orchestrates cryptic transcript suppression and transcriptional directionalityCommunications BiologyOctober 22, 2024Haejin An, Hyeokjun Yang, Daeyoup LeeEDTA, 1% SDS) at 65 °C for 1 h. Subsequently, RNase A (ThermoFisher, EN0531) and proteinase K (Promega, V3021) were added to the eluted DNA for RNA degradation and de-crosslinking. The extracted DNA was purified

(17) An In Vivo Model of Propionic Acid-Rich Diet-Induced Gliosis and Neuro-Inflammation in Mice (FVB/N-Tg(GFAPGFP)14Mes/J): A Potential Link to Autism Spectrum DisorderInternational Journal of Molecular SciencesJuly 25, 2024Piotr P. Lagod, Latifa S. Abdelli, Saleh A. NaserDNA isolated from tail snips. The tail snips were treated with lysis buffer (0.4 mg/mL proteinase K [V3021, Promega, Madison, WI, USA] 10 mM Tris pH 8.00, 100 mM NaCl, 10 mM Ethylenediaminetetraacetic acid [EDTA],

(18) Hyaluronidase inhibitor delphinidin inhibits cancer metastasisScientific ReportsJune 28, 2024Jeremy McGuire, Taketo Taguchi, Gregory Tombline, Victoria Paige, Michelle Janelsins, Nikesha Gilmore, Andrei Seluanov, Vera Gorbunovarange of delphinidin. Ten ml of conditioned media was treated with 1 ml Proteinase K (Promega Cat # V3021) in buffer (10 mM Tris-Cl pH8.0, .25 mM EDTA, 100 mM NaCl, 0.5% SDS 1 mg/ml proteinase K) (Roche Cat

(19) Zebrafish polg2 knock-out recapitulates human POLG-disorders; implications for drug treatmentCell Death & DiseaseApril 20, 2024Raquel Brañas Casas, Alessandro Zuppardo, Giovanni Risato, Alberto Dinarello, Rudy Celeghin, Camilla Fontana, Eleonora Grelloni, Alexandru Ionut Gilea, Carlo Viscomi, Andrea Rasola, et al.lysis buffer (100 mM Tris HCl pH 8-8.5, 200 mM NaCl, 0.2% SDS, 5 mM EDTA) and proteinase K (10 mg/mL, V3021, Promega), at -20 °C. After the genotyping, individuals were grouped together by genotype, creating

(20) A single cell atlas of the mouse seminal vesiclebioRxivApril 15, 2024Fengyun Sun, Kathleen Desevin, Yu Fu, Shanmathi Parameswaran, Jemma Mayall, Vera Rinaldi, Nils Krietenstein, Artur Manukyan, Qiangzong Yin, Carolina Galan, et al.(3 × 5 min) and a second antigen retrieval step at 37°C using proteinase K (20 μg/ml in TBS, pH 8, V3021, Promega), the slides were blocked as described above. This was followed by a second primary antibody

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Proteinase K

Proteinase K from Promega

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Proteinase K from Promega

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