Fig 1: Role of FZD7 in Wnt5a-induced gemcitabine resistance in Capan-2 cells. (A-a) rhWnt5a treatment enhances the IC50 of Gem in Capan-2 cells. (A-b) Fc, an inhibitor of FZD7, attenuates the increase in IC50 of Gem induced by rhWnt5a. (A-c) rhWnt5a does not enhance IC50 of Gem in Capan-2 cells siRNA silencing of FZD7 expression. (B-a and B-b) Gem increases the number of late apoptotic and necrotic cells in Capan-2 cells. (B-c) rhWnt5a treatment decreases the number of late apoptotic and necrotic cells following Gem treatment. (B-d) Effect of rhWnt5a on Gem-induced cell death is reduced following Fc treatment. (B-e) Quantification of results. (C-a and C-b) rhWnt5a treatment reduces the number of apoptotic and dead cells in conCapan-2 cells induced by Gem. (C-c) FZD7 siRNA silencing inhibits rhWnt5a-induced Gem resistance. (C-d) Quantification of results. **P<0.01, ***P<0.001. rhWnt5a, recombinant human Wnt5a; FDZ7, Frizzled class receptor 7; siRNA, small interfering RNA; con, control; veh, vehicle.
Fig 2: Wnt5a regulates the expression of ABCG2 gene through FZD7. Data sets of ‘Tumor Pancreatic adenocarcinoma-TCGA-178-rsem-tcgars’, ‘Tumor Pancreatic ductal adenocarcinoma-Yeh-132-custom-4hm44k’ and ‘Tumor Pancreatic ductal adenocarcinoma (ICGC)-Perez-91-complex-ilmnht12v4’ in the R2 database demonstrated the correlation between Wnt5a and FZD7 in pancreatic carcinoma (A-a) correlation between FZD7 and ABCG2 in pancreatic carcinoma (A-b). (B-a) Protein levels of FZD7 in four pancreatic cancer cell lines. (B-b) Protein levels of FZD7 after transient transfection of different siRNA molecules targeting FZD7. (C) rhWnt5a does not upregulate ABCG2 and β-catenin expression following transfection with siFZD7. (D) ABCG2 protein expression following FZD7 overexpression in Capan-2 cells treated with rhWnt5a. *P<0.05, **P<0.01, ***P<0.001. ABCG2, ATP binding cassette subfamily G member 2; rhWnt5a, recombinant human Wnt5a; FDZ7, Frizzled class receptor 7; siRNA, small interfering RNA; con, control.
Supplier Page from R&D Systems, a Bio-Techne Brand for Recombinant Human Frizzled-7 Fc Chimera Protein, CF