Fig 1: The late upregulated genes Sema6a, Ntng2, and TGF-β1, but not GDF7, partially rescue neurite outgrowth under inhibitory conditions and are expressed in the spinal GM at late stages after stroke. A, Representative pictures of N1E-115 cells 24 h after addition of control medium (Ctrl), SCE, or the combination of SCE and Sema6a/Ntng2/GDF7/TGF-β1 protein, respectively. Quantification of mean neurite outgrowth per cell is normalized to medium control condition. B, In situ hybridization with subsequent analysis of signal intensity of the mRNA in four selected regions within the stroke-denervated hemicord 28 dpi and after sham operation. Representative pictures of in situ hybridization for Sema6a and Ntng2 in the ventral horn and TGF-β1 in the dorsal funiculus. Scale bar, 50 μm. *P < 0.05, **P < 0.01, ***P < 0.001.