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T4 Polynucleotide Kinase
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T4 Polynucleotide Kinase
T4 Polynucleotide Kinase from New England Biolabs
(1)
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Citations:
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New England Biolabs for
T4 Polynucleotide Kinase
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Description
T4 Polynucleotide Kinase
Product Specs
Company Info
Citations
Reviews
Protocols
Product Specs
Item
T4 Polynucleotide Kinase
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
M0201S
Format
10 u/µl
Molecule Name
Polynucleotide Kinase
Activity
One Richardson unit is defined as the amount of enzyme catalyzing the incorporation of 1 nmole of acid-insoluble [32P] in 30 minutes at 37°C.
Quantity
500 units
Applications
DNA Manipulation
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Enzymes
Temperature
-20 C
Add to Compare List
New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (1124)
(1)
Hfq protein and GcvB small RNA tailoring of oppA target mRNA to levels allowing translation activation by MicF small RNA in Escherichia coli
RNA Biology
December 31, 2023
Marie-Claude Carrier, David Lalaouna, Eric Massé
methods Reagents Enzymes EcoRI (R3101), BamHI (R3136), SphI (R3182), MscI (R0534),
T4 Polynucleotide kinase
(
M0201
), Protoscript II (
M0368
), calf intestinal phosphatase (M0290),
Q5 High fidelity DNA polymerase
(
M0491
),
(2)
An impaired ubiquitin-proteasome system increases APOBEC3A abundance
NAR Cancer
December 19, 2023
Margo Coxon, Madeline A Dennis, Alexandra Dananberg, Christopher D Collins, Hannah E Wilson, Jordyn Meekma, Marina I Savenkova, Daniel Ng, Chelsea A Osbron, Tony M Mertz,
et al.
plasmids Oligos with
shRNA
sequences (listed in Supplementary Table S1) were phosphorylated with T4 PNK (
NEB
M0201S
) and annealed. To create the lentiviral
shRNA
plasmids, the annealed oligos were cloned into the
AgeI
(3)
Extending the toolbox for RNA biology with SegModTeX: a polymerase-driven method for site-specific and segmental labeling of RNA
Nat Commun
December 18, 2023
Raphael Haslecker, Vincent V. Pham, David Glänzer, Christoph Kreutz, Theodore Kwaku Dayie, Victoria M. D'Souza
DNA oligo (5’TAATACGACTCACTATAGGGTCTCTTGTTCATGAGTCATGG3’) was 5’ phosphorylated with T4 PNK (
NEB
, #
M0201S
) and ligated onto the 3’ ends of PAGE purified SegModTeX and T7 constructed 7SK snRNA NMR samples with
(4)
Programmable modulation of ribosomal frameshifting by mRNA targeting CRISPR-Cas12a system
iScience
December 15, 2023
Shih-Hong Huang, Shih-Cheng Chen, Tsu-Ying Wu, Cheng-Yao Chen, Chien-Hung Yu
M0491S
T4 DNA Ligase
,
New England Biolabs
,Cat#
M0202L
T4 Polynucleotide Kinase
,
New England Biolabs
,Cat#
M0201L
HiFi DNA Assembly
Cloning
Kit,
New England Biolabs
,Cat#
E5520S
T7 RNA Polymerase
,
New England Biolabs
,Cat#
(5)
A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genes
Elife
December 14, 2023
Nicole A. Ellis, Kevin S. Myers, Jessica Tung, Anne Davidson Davidson Ward, Kathryn Johnston, Katherine E. Bonnington, Timothy J. Donohue, Matthias P. Machner
combined 1 µL:1 µL and were phosphorylated in a 50 µL reaction using
T4 Polynucleotide Kinase
(
NEB
M0201L
) for 2 hr at 37°C. The reaction was stopped by addition of NaCl to a final concentration of 50 mM. Next,
(6)
The Effect of Pseudoknot Base Pairing on Cotranscriptional Structural Switching of the Fluoride Riboswitch
bioRxiv
December 5, 2023
Laura M Hertz, Elise N White, Konstantin Kuznedelov, Luyi Cheng, Angela M Yu, Rivaan Kakkaramadam, Konstantin Severinov, Alan Chen, Julius B Lucks
T4 DNA Ligase Reaction Buffer
(
NEB
, #B0202A), 1 μL T4 Ligase (
NEB
, #
M0202L
), and 1 μL T4 PNK (
NEB
, #
M0201L
) for 30 to 60 min at room temperature. The ligated product was then transformed into NEBTurbo competent
(7)
Protocol for the saturation and multiplexing of genetic variants using CRISPR-Cas9
STAR Protocols
December 1, 2023
Sounak Sahu, Teresa Sullivan, Eileen Southon, Dylan Caylor, Josephine Geh, Shyam K. Sharan
P4394
Olaparib,Selleck Chemicals,Cat# AZD2281
T4 polynucleotide kinase
enzyme,
New England Biolabs
,Cat#
M0201S
BbsI
(high fidelity) enzyme,
New England Biolabs
,Cat#
R0539S
T7 DNA ligase
enzyme,
New England Biolabs
,Cat#
(8)
Protocol for arrayed gRNA screening by base editors in mammalian cell lines using lentiviral system
STAR Protocols
December 1, 2023
Nithin Sam Ravi, Anila George, Kumarasamypet M. Mohankumar
proteins" ,, GoTaq G2 Hot Start Master Mix,
Promega
,CAT #
M7422
T4 polynucleotide kinase
,
NEB
,CAT #
M0201
T4 DNA ligase
,
NEB
,CAT #
M0202
BsmB1,NEB,CAT # R0580 Polyethylene glycol,Sigma-Aldrich,CAT # 89510 Lenti-X
(9)
SLC3A2 N-glycosylation and Golgi remodeling regulate SLC7A amino acid exchangers and stress mitigation
Journal of Biological Chemistry
December 1, 2023
Cunjie Zhang, Massiullah Shafaq-Zadah, Judy Pawling, Geoffrey G. Hesketh, Estelle Dransart, Karina Pacholczyk, Joseph Longo, Anne-Claude Gingras, Linda Z. Penn, Ludger Johannes,
et al.
CRISPR/CAS9
Reagents:
FastDigest
BbsI (#
FD1014
) from
Thermo Scientific
;
T7 DNA ligase
(#
M0318S
), T4 PNK (#
M0201S
),
ATP
(#
P0756S
) from
NEB
; Plasmid-Safe ATP-Dependent DNase (#E3101K) from Epicentre; PolyJet reagent
(10)
Rfoot‐seq: Transcriptomic RNase Footprinting for Mapping Stable RNA‐Protein Complexes and Rapid Ribosome Profiling
Current Protocols
November 20, 2023
Qianru Li, Emily K. Stroup, Zhe Ji
T4 polynucleotide kinase reaction buffer
(
New England BioLabs
, cat. no.
B0201S
)
T4 Polynucleotide kinase
(
New England BioLabs
, cat. no.
M0201L
). SUPERase•In RNase inhibitor (Invitrogen, cat. no. AM2694) 5× first strand buffer (
Invitrogen
, cat.
(11)
Replication‐induced DNA secondary structures drive fork uncoupling and breakage
The EMBO Journal
November 15, 2023
Sophie L Williams, Corella S Casas‐Delucchi, Federica Raguseo, Dilek Guneri, Yunxuan Li, Masashi Minamino, Emma E Fletcher, Joseph TP Yeeles, Ulrich F Keyser, Zoë AE Waller,
et al.
labelled with γ‐32P‐ATP in a reaction containing 5 pmol oligo, 1× PNK buffer, 1 U of PNK enzyme (
NEB
,
M0201S
), and γ‐32P‐ATP (0.03 mCi). The reaction was incubated for 1 h at 37°C, followed by heat inactivation
(12)
Noodles, the all-in-one system for on-target efficiency analysis of CRISPR guide RNAs
MethodsX
November 15, 2023
Dongfa Lin, Syeda Sadia Najam, Yu Liu, Nicola Murgia, Ilya A. Vinnikov
oligonucleotide (see Table 1).•Antisense oligonucleotide.•
T4 phage
polynucleotide kinase (PNK,
NEB
,
M0201S
, see Table S1 for detailed information about reagents).•
T4 phage
ligation
buffer (
NEB
:
B0202S
).•ddH2O
(13)
Efficient 3′-pairing renders microRNA targeting less sensitive to mRNA seed accessibility
Nucleic Acids Research
November 10, 2023
David M Kosek, Elnaz Banijamali, Walter Becker, Katja Petzold, Emma R Andersson
purchased from IDT. Inserts were phosphorylated by
incubation
with
T4 polynucleotide kinase
(
NEB
, #
M0201
) in T4 DNA ligase buffer (NEB, #B0202) at 37ºC for 30 min, and then annealed by heating to 95ºC for
(14)
Synthetic chromosome fusion: Effects on mitotic and meiotic genome structure and function
Cell Genomics
November 8, 2023
Jingchuan Luo, Luis A. Vale-Silva, Adhithi R. Raghavan, Guillaume Mercy, Jonna Heldrich, Xiaoji Sun, Mingyu Kenneth Li, Weimin Zhang, Neta Agmon, Kun Yang,
et al.
dNTP Mixture
(each 2.5 mM),Takara,4030 "
T4 Polynucleotide Kinase
(10,000 units/ml)",
New England BioLabs
,
M0201S
Klenow Fragment,
New England BioLabs
,
M0210S
T4 DNA Polymerase
,
New England BioLabs
,
M0203S
dATP (100 mM
(15)
PRO-IP-seq tracks molecular modifications of engaged Pol II complexes at nucleotide resolution
Nature Communications
November 3, 2023
Anniina Vihervaara, Philip Versluis, Samu V. Himanen, John T. Lis
45 min. The 5'-hydroxyrepair was subsequently conducted by adding
T4 polynucleotide kinase
mix (
NEB
,
M0201S
) and maintaining the samples at 37 °C for additional 45 min. The bead-nascent-RNA-complexes were washed
(16)
Targeting histone deacetylase suppresses tumor growth through eliciting METTL14-modified m(6) A RNA methylation in ocular melanoma
Cancer communications (London, England)
November 1, 2023
Zhuang A, Gu X, Ge T, Wang S, Ge S, Chai P, Jia R, Fan X.
washing, the samples on the beads were subjected to dephosphorylation with
T4 polynucleotide kinase
(
M0201L
,
New England Biolabs
, Ipswich, MA, USA) at 37°C for 20 min. The RNA was then purified, subjected to
(17)
SND1 binds SARS-CoV-2 negative-sense RNA and promotes viral RNA synthesis through NSP9
Cell
October 26, 2023
Nora Schmidt, Sabina Ganskih, Yuanjie Wei, Alexander Gabel, Sebastian Zielinski, Hasmik Keshishian, Caleb A. Lareau, Liv Zimmermann, Jana Makroczyova, Cadence Pearce,
et al.
proteins" ,, Dynabeads Protein G,
Thermo Fisher Scientific
,
10004D
T4 Polynucleotide Kinase
,
New England Biolabs
,
M0201L
FastAP Thermosensitive
Alkaline Phosphatase
,
Thermo Fisher Scientific
,
EF0651
100% DMSO,Sigma-Aldrich,41639 50%
(18)
Integrative genome-scale analyses reveal post-transcriptional signatures of early human small intestinal development in a directed differentiation organoid model
BMC Genomics
October 26, 2023
Yu-Han Hung, Meghan Capeling, Jonathan W. Villanueva, Matt Kanke, Michael T. Shanahan, Sha Huang, Rebecca Cubitt, Vera D. Rinaldi, John C. Schimenti, Jason R. Spence,
et al.
Ipswich, MA; M0356), (iv) 5′ end phosphorylation using
T4 polynucleotide kinase
(
NEB
, Ipswich, MA;
M0201
), (iv) 5′ adapter
ligation
with T4 RNA Ligase 1 (NEB, Ipswich, MA;
M0204
). The 5’ adaptor contained
(19)
XPOT Disruption Suppresses TNBC Growth through Inhibition of Specific tRNA Nuclear Exportation and TTC19 Expression to Induce Cytokinesis Failure
International Journal of Biological Sciences
October 24, 2023
Huijuan Dai, Xiaomei Yang, Xiaonan Sheng, Yaohui Wang, Shan Zhang, Xueli Zhang, Lipeng Hu, Zhigang Zhang, Xinrui Dong, Wenjin Yin,
et al.
tRNA was purified with TRIzol reagent and then re-phosphorylated with 10 U
T4 polynucleotide kinase
(
M0201L
;
New England Biolabs
) polynucleotide kinase in a 20-µL reaction mixture containing 70 mM Tris-HCl (pH
(20)
Transcriptome-wide analysis of the function of Ded1 in translation preinitiation complex assembly in a reconstituted in vitro system
bioRxiv
October 20, 2023
Fujun Zhou, Julie M. Bocetti, Meizhen Hou, Daoming Qin, Alan G. Hinnebusch, Jon R. Lorsch
fragments purified from 48S PIC RPFs were dephosphorylated using T4 Polynucleotide Kinase (PNK) (
NEB
;
M0201L
) and ligated to pre-adenylated linkers (NI-810 to NI-815) containing 5 nt sample barcodes unique for
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Reviews
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(1)
4/19/2016
T4 Kinase Kit
Benjamin Gerovac
This kit is used to add 5' phosphate groups to digested DNA so it can be ligated and cloned.
<<
<
>
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Protocols
Genome- Scale Mapping of DNase I Hypersensitivity
July 01, 2013
In Vitro Transcription and Immobilized Template Analysis of Preinitiation Complexes
January 01, 2012
In Vitro Selection of RNA Aptamers to a Protein Target by Filter Immobilization
October 01, 2009
Serial Analysis of Gene Expression (SAGE): Experimental Method and Data Analysis
October 01, 2007
Construction of Bacterial Artificial Chromosome (BAC/PAC) Libraries
July 01, 2001
Purification of Sequence- Specific DNA- Binding Proteins by Affinity Chromatography
October 01, 1993
T4 Polynucleotide Kinase from New England Biolabs
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