Insulin, human recombinant, zinc solution 12585014 from Thermo Fisher Scientific

Product Specs
ItemInsulin, human recombinant, zinc solution
CompanyThermo Fisher Scientific
Price $41.50Supplier Page View Company Product Page
Catalog Number12585014
Conjugate/TagUnconjugated
SpeciesHuman
Activity4 mg/mlPotency: (dry basis) >= 27 USP units/mg.Suggested range for supplementation: 2 to 20 µg/ml
Molecule NameInsulin
Source/Expression SystemRecombinant
Regulatory StatusFor Research Use Only. Not for use in diagnostic procedures
Size4 mg/ml

Additional Product Information

Protein/Peptide:
Insulin

Protein/Peptide Species:
Human

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

Company Profile

Website: www.thermofisher.com

(1) Development of a GPCR-binding ligand screening method using real-time interaction analysis on live cellsBiochemistry and Biophysics ReportsDecember 1, 2026Hadis Westin, Hanna Yushchyshyna, Olga Vorontsova, Saloni Chopra, Tianqi Xu, Christoffer Bengtsson, Fabio Begnini, Preeti Jha, Ulrika Rosenström, Ulrika Yngve, et al.and 5% FBS, respectively. OVCAR3 medium also contained 0.01 mg/mL insulin (ThermoFisher/Gibco, Cat# 12585014). PANC1 was cultured in DMEM supplemented with 10% FBS and 1% PEST. Cells were grown in tissue culture

(2) USP25 promotes ferroptosis in diabetic retinopathy by deubiquitinating p53 and suppressing the GPX4/SLC7A11 axisJ Diabetes InvestigOctober 27, 2026Yanjun Gao, Wenjing Zhao, Qiannan Chai, Cunxi Yemetabolic derangement while maintaining a stable chronic hyperglycemic state, 0–0.2 units of insulin (12585014, Invitrogen, Carlsbad, CA, USA) were administered intraperitoneally every 2–3 days based on individual

(3) Pharmacologically Targeting DHODH-Dependent Pyrimidine Metabolism with HSA-Conjugated BRQ Suppresses LeukemogenesisCancers (Basel)September 23, 2026Xiaoxu Zhang, Changhao Tian, Yang Ding, Lei Dong, Jia Yu, Rui Su, Min Gaosodium pyruvate (113600 70, Thermo Fisher Scientific, Waltham, MA, USA), and 10 mg/mL human insulin (12585014, Thermo Fisher Scientific, Waltham, MA, USA). The AML PDX-148 cells were cultured in IMDM (12440061,

(4) Manganese availability determines insulin sensitivity by enhancing Akt activitybioRxivSeptember 17, 2026Jennifer R. Gamarra, Sei Higuchi, Timothy L. Yuan, Yuke Xie, Cong Liu, Niroshan Shanmugarajah, Hang Yang, Meredith O. Kelly, Sarah A. Hannou, Kathrin Schilling, et al.#J63292MA) and 1 μM dexamethasone (Sigma #D2915) was added for 5 hours. 1 or 100 nM of insulin (Gibco #12585014) was then added for 3 minutes. Cells were washed 2x with ice cold PBS before direct cell lysis with

(5) Receptor profiling and growth assessment of influenza A virus in porcine mammary and non-mammary tissues and derived cellsJournal of VirologyAugust 18, 2026Ulises Barron-Castillo, Nathalie Berube, Cynthia L. Swan, M. Afzal Javed, Lauren Aubrey, Jill Trann, Makenzie Gidych, Sauhard Shrivastava, Kaushal Baid, Arinjay Banerjee, et al.DMEM/F12 (Thermo Fisher Scientific, 12634010) supplemented with 10% FBS (Sigma), 5 μg/mL insulin (GIBCO, 12585014), 1 μg/mL hydrocortisone (Stemcell Technologies, 07926), 5 ng/mL epidermal growth factor (EGF, GIBCO,

(6) Decoding gene responsiveness to synthetic chromatin reader-actuators with multi-modal epigenomic profilingnpj Systems Biology and ApplicationsAugust 3, 2026Seong Hu Kim, Isioma Enwerem-Lackland, Natecia L. Williams, Rachel Fisher, Christopher Plaisier, Karmella A. Haynesglucose DMEM (Gibco #11965092), 20% tet system approved FBS (Gibco #A4736401), 0.01 mg/mL insulin (Gibco #12585014), and 2 mM L-Glutamine (Gibco #25030081), with 5% CO2 in a humidified incubator. 1X DPBS without calcium

(7) Estrogen-Driven Breast Carcinogenesis in a Rat Model is Linked to Retroelement Activation and Inflammation and is Susceptible to Lamivudine TreatmentBreast Cancer (Dove Med Press)July 23, 2026Valentina Evdokimova, Zhenbo Zhang, Peter Ruzanov, Minsheng Hung, Naghmeh Khoshgoo, Suky Zheng, Mary Anne Quintayo, Lincoln D Stein, Jane Bayani, Laszlo Radvanyi24–48 h in the phenol red-free MEM (MCF7) or RPMI (T47D) medium supplemented with 10 µg/mL insulin (#12585014, Fisher Scientific) and 10% charcoal stripped fetal bovine serum (FBS) (#A3382101, Fisher Scientific),

(8) Neural dynamics of temporal interference stimulation monitoring by soft liquid metal interfaces across neural systemsNature CommunicationsJuly 20, 2026Enji Kim, Wonjung Park, Yeon-Mi Hong, Jungsoo Hong, Inhea Jeong, Jakyoung Lee, Sanghoon Lee, Hyunjin Lee, Hyun-Mun Kim, Noviana Wulansari, et al.without vitamin A (Gibco, 12587-010), 1% GlutaMAX, 0.5% MEM-NEAA, 0.025% human insulin solution (Gibco, 12585014), 50 μM β-mercaptoethanol (Invitrogen), and 1% Penicillin/Streptomycin (Gibco,15140122)]. Organoids

(9) Reduced SH3RF3 May Protect Against Alzheimer's Disease by Lowering Microglial Pro‐Inflammatory Responses via Modulation of JNK and NFkB SignalingGliaJuly 1, 2026Ronak Patel, Rong Cheng, Christopher L. Cardona, Ellen Angeles, Gunjandeep Singh, Sabrina Miller, Archana Ashok, Andrew F. Teich, Angel Piriz, Aleyda Maldonado, et al.acids,ThermoFisher,cat.# 11140050 Insulin‐transferrin‐selenium,ThermoFisher,cat.# 41400045 Insulin,Sigma‐Aldrich,cat.# 12585014 Glutamax,ThermoFisher,cat.# 35050061 B27‐ supplements,ThermoFisher,cat.# 17504044 N2‐ supplements,ThermoFisher,cat.#

(10) Targeting arginine metabolism overcomes chemotherapy resistance in aggressive-variant prostate cancersiScienceJune 19, 2026Elavarasan Subramani, Patrick G. Pilié, Rebecca Slack-Tidwell, Paul V. Viscuse, Xianghong Kuang, Thirukumaran Kandasamy, Dominik Awad, Jenny J. Han, Licai Huang, Christine B. Peterson, et al.,Selleck Chemicals,S2840 Matrigel® Matrix,Corning,356231 RPMI 1640,Corning,10-040-CV FBS,Sigma,F4135 Insulin,Gibco,12585014 Apo-Transferrin,Sigma,T5391 Sodium selenite,Sigma,S5261 L-Glutamine,Gibco,25030081 Sodium Pyruvate,Gibco,11360070 HEPES solution

(11) Pathological extracellular matrix changes in decellularized normal appearing gray matter and subpial multiple sclerosis lesionsiScienceJune 19, 2026Jody M. de Jong, Justina C. Wolters, Marion H.C. Wijering, Joop de Vries, Susanne M. Kooistra, Bart J.L. Eggen, Wia BaronThermofisher Scientific), 2-mercaptoethanol (1 μg/mL, #M3148, Sigma-Aldrich), insulin (100 μg/mL, #12585014, Gibco)), supplemented with 100 nM retinoic acid (#R2625, Sigma-Aldrich), 10 μm SB-431542 (#1614, R&D Systems

(12) Engineering functional ventral midbrain dopaminergic neurons in human organoids through WNT modulation and bioreactor cultureMolecular PsychiatryJune 17, 2026Hariam Raji, Federico Bertoli, Maria Jose Perez, Alicia Lam, Laura Volpicelli-Daley, Michela DeleidiGlutaMax (Gibco, Cat # 35050061), 1% NEAA, 0.1% β-mercaptoethanol, 2.5 µg/mL insulin (Gibco, Cat # 12585014), 200 ng/mL laminin (Thermo Fisher, Cat # 23017015), 7.5 µM CHIR99021, 100 ng/mL SHH-C25II, 1 µM purmorphamine,

(13) Engineered microtissue systems for identifying the roles of Wnt and YAP signaling in hepatoblast differentiation and organizationMater Today BioJune 11, 2026Brock Grenci, Ariane Tsai, Katie Zobus, Gregory H. Underhillpenicillin/streptomycin (Cytiva SV30010), 1% L-glutamine (Cytiva SH30034.01), 10ug/mL Human Recombinant Insulin (Gibco 12585014), 30 ng/mL IGF-2 (PeproTech 100-12), and 50 ng/mL EGF (PeproTech AF-100-15). Cells were either passaged

(14) A proteogenomic RNA processing mechanism drives sex differences in meningiomaResearchSquareJune 2, 2026David Raleigh, Martha Cady, Ayush Aggarwal, Nicholas Stevers, Naomi Zakimi, Isabelle Liu, Kanish Mirchia, John Coukos, Pornparn Kongpracha, Yuan Zhou, et al.RPMI (11875093, Thermo Scientific) supplemented with 10% FBS and human recombinant insulin 0.01mg/mL (12585014, Thermo Scientific). MCF7 breast cancer cells were cultured in EMEM (M4655, Sigma) supplemented with

(15) Scaling human liver microphysiological systems: implementing a higher-throughput liver acinus microphysiological system platformExperimental Biology and MedicineMay 22, 2026Dillon C. Gavlock, Michael W. Castiglione, Allen Wang, Mahboubeh Varmazyad, Lawrence A. Vernetti, Mark E. Schurdak, D. Lansing Taylor, Jacquelyn A. Brown, Mark T. Miedelsupplemented with physiologically relevant levels of glucose (MilliporeSIgma, G8644), insulin (ThermoFisher, 12585014), and glucagon (MilliporeSIgma and molecular drivers of fibrosis including TGF-β1 (ThermoFisher, PHG9214)

(16) Repurposing Artesunate to Combat Progression and Metastasis via Targeting Circulating Tumor CellsOncology ResearchMay 21, 2026Evangelia Pantazaka, Dimitrios Papakonstantinou, Argyro Roumeliotou, Dafni Graikioti, Sotirios Tsakas, Nefeli Zacharopoulou, Stuart S. Martin, Athanasios Kotsakis, Constantinos M. Athanassopoulos, Catherine Alix-Panabières, et al.cells, were cultured as described above with the addition of 0.01 mg/mL human recombinant insulin (12585014, Thermo Fisher Scientific). DMS 454 (95062832, RRID: CVCL_2438), SW-620 (CCL-227, RRID: CVCL_0547) and

(17) Media matters: phenol red and fetal bovine serum estrogen in traditional cell culture media influence human mesenchymal stromal cell (hMSC) processes and differentiation in a sex-biased mannerBiology of Sex DifferencesMay 19, 2026John C. Bradford, Jennifer L. RobinsonA17590.03), 500 μm Isobutylmethylxanthine (Thermo Scientific Chemicals # J64598.MC), 5 ug/ml Insulin (Gibco# 12585014), and 200 ug/ml of Ascorbic Acid (Sigma-Aldritch# 49752). Image analysis was performed utilizing specifically

(18) Ras promotes macropinocytic nutrient uptake by suppressing the albumin recycling receptor FcRnEMBO ReportsApril 29, 2026Rafael Paschoal de Campos, Xuxia Wu, Aslihan Inal, Zhao Liu, Craig B Thompson, Wilhelm Palm,Thermo Fisher Scientific,450-33 Mouse PDGF-BB Recombinant Protein,Life Technologies,PMG0044 Insulin,Life Technologies,12585014 BamHI-HF,NEB,R3136 BbsI-HF,NEB,R3539 FastDigest Esp3I,Thermo Fisher Scientific,FD0454 Q5 High-Fidelity DNA Polymerase

(19) Unraveling the Molecular Mechanisms Underlying Spontaneous Multipolar Mitosis Through CIN‐seqAdvanced ScienceApril 27, 2026Pin‐Rui Su, Ting‐Chun Chou, Maria Teresa López‐Cascales, Junfeng Huang, Tsai‐Ying Chen, Sjoukje van Wieren, Chan Li, Cecile Beerens, Li You, Jelle Storteboom, et al.horse serum (Fisher Scientific, SH3007403), 1% Penicillin/Streptomycin, 1% (w:w) insulin (ThermoFisher, 12585014), 0.05% (w:w) hydrocortisone (Stem Cell, 74142), 0.01% (w:w) cholera toxin (Sigma, SAE0069) and 0.001%

(20) A MERTK-Targeting Antibody-Drug Conjugate Selectively Depletes M2 Tumor-Associated Macrophages and MERTK-Expressing Cancer CellsCancer researchApril 15, 2026Takeda S, Sridhar S, Schefer D, Andreu-Agullo C, Lo PC, Lee M, Busby R, Darst DM, Assmus A, Anaganti S, et al.BT-474 were cultured in RPMI-1640 medium containing 20% Fetal Bovine Serum, 10 μg/mL insulin (Gibco, 12585014) and 1% penicillin-streptomycin. MDA-MB-231 and MDA-MB-231-LM2 cells were cultured in DMEM (ATCC, 30-2002)

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Insulin, human recombinant, zinc solution from Thermo Fisher Scientific

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Insulin, human recombinant, zinc solution from Thermo Fisher Scientific

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